##################################
# NO CUT ENZYMES ( 64 enzymes) #
##################################
AatII AhdI Aor51HI ApaI AscI
AspEI AspI AviII BbeI BbrPI
BcgI BglI BsiEI BsrBI BssHII
Bst1107I BstEII BstPI BstZ17I CfoI
CpoI DsaI EagI Eam1105I EclXI
Eco47III Eco52I EcoO65I FseI FspI
HaeII HhaI HinP1I KasI KspI
MluI NaeI NarI NcoI NgoMIV
NheI NotI NruI PacI PaeR7I
PflFI PmaCI PmeI PmlI PvuI
RsrII SacII SalI SbfI SfiI
SfoI SgrAI SnaBI SpeI SphI
Sse8387I SwaI Tth111I XhoI
###################################
# ONE CUT ENZYMES ( 41 enzymes) #
###################################
Acc65I 4615 GGTACC G^GTACC
AccII 6295 CGCG CG^CG
AccIII 4071 TCCGGA T^CCGGA
AclI 2432 AACGTT AA^CGTT
AcyI 4989 GGCGTC G[GA]^CG[CT]C
AgeI 10170 ACCGGT A^CCGGT
Alw44I 701 GTGCAC G^TGCAC
ApaLI 701 GTGCAC G^TGCAC
Asp718I 4615 GGTACC G^GTACC
AvrII 5726 CCTAGG C^CTAGG
BalI 6407 TGGCCA TGG^CCA
BciVI 1993 GGATAC GTATCC(6/5)
BlnI 5726 CCTAGG C^CTAGG
BlpI 8987 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 8987 GCTGAGC GC^T[ACGT]AGC
BsaAI 2416 TACGTG [CT]AC^GT[GA]
BsaHI 4989 GGCGTC G[GA]^CG[CT]C
BseAI 4071 TCCGGA T^CCGGA
BsgI 8292 CTGCAC GTGCAG(16/14)
BsiWI 2414 CGTACG C^GTACG
BspDI 3767 ATCGAT AT^CGAT
BspEI 4071 TCCGGA T^CCGGA
BstUI 6295 CGCG CG^CG
CelII 8987 GCTGAGC GC^T[ACGT]AGC
ClaI 3767 ATCGAT AT^CGAT
DrdI 6352 GACACCAGAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
FauI 7474 GCGGG CCCGC(4/6)
Hin1I 4989 GGCGTC G[GA]^CG[CT]C
HpaI 260 GTTAAC GTT^AAC
KpnI 4615 GGTACC GGTAC^C
MluNI 6407 TGGCCA TGG^CCA
MroI 4071 TCCGGA T^CCGGA
MscI 6407 TGGCCA TGG^CCA
MvnI 6295 CGCG CG^CG
PinAI 10170 ACCGGT A^CCGGT
PshAI 3819 GACGTGGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Psp1406I 2432 AACGTT AA^CGTT
SexAI 1645 ACCTGGT A^CC[AT]GGT
SmaI 7135 CCCGGG CCC^GGG
XmaCI 7135 CCCGGG C^CCGGG
XmaI 7135 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AciI 7911 CCGC CCGC(-3/-1)
7474 GCGG
AocI 665 CCTCAGG CC^T[ACGT]AGG
9481 CCTGAGG
AseI 7055 ATTAAT AT^TAAT
8335 ATTAAT
AsnI 7055 ATTAAT AT^TAAT
8335 ATTAAT
BamHI 1854 GGATCC G^GATCC
5353 GGATCC
BbvCI 8867 CCTCAGC CCTCAGC(-5/-2)
3089 GCTGAGG
Bsp1407I 8065 TGTACA T^GTACA
9446 TGTACA
BspLU11I 1382 ACATGT A^CATGT
3446 ACATGT
BspMI 4134 ACCTGC ACCTGC(4/8)
4846 ACCTGC
BsrGI 8065 TGTACA T^GTACA
9446 TGTACA
BssSI 1269 CACGAG CACGAG(-5/-1)
4326 CACGAG
BstBI 3699 TTCGAA TT^CGAA
4437 TTCGAA
Bsu36I 665 CCTCAGG CC^T[ACGT]AGG
9481 CCTGAGG
DraIII 2690 CACAAGGTG CAC[ACGT][ACGT][ACGT]^GTG
5561 CACGGGGTG
EaeI 4180 TGGCCG [CT]^GGCC[GA]
6407 TGGCCA
Eco81I 665 CCTCAGG CC^T[ACGT]AGG
9481 CCTGAGG
EcoRV 3764 GATATC GAT^ATC
9002 GATATC
NspV 3699 TTCGAA TT^CGAA
4437 TTCGAA
PpuMI 852 AGGACCT [GA]G^G[AT]CC[CT]
2356 AGGACCT
PshBI 7055 ATTAAT AT^TAAT
8335 ATTAAT
SacI 3047 GAGCTC GAGCT^C
4230 GAGCTC
SapI 1099 GAAGAGC GCTCTTC(1/4)
1829 GAAGAGC
SfuI 3699 TTCGAA TT^CGAA
4437 TTCGAA
SspBI 8065 TGTACA T^GTACA
9446 TGTACA
XbaI 1724 TCTAGA T^CTAGA
5755 TCTAGA
#########################################
# 3 OR MORE CUT ENZYMES ( 128 enzymes) #
#########################################
AccI AcsI AfaI AflII AflIII
AluI AlwI AlwNI ApoI Asp700I
AspHI AvaI AvaII BanI BanII
BbsI BbvI BclI BcnI BfaI
BfrI BglII BmyI BpmI BpuAI
BsaBI BsaI BsaJI BsaWI BseRI
BsiHKAI BsiYI BslI BsmAI BsmBI
BsmFI BsmI BsoBI Bsp1286I BspHI
BsrDI BsrFI BsrI BssKI BstAPI
BstF5I BstNI BstXI BstYI Cac8I
Cfr10I Cfr13I DdeI DpnII DraI
DraII EarI EcoNI EcoO109I EcoRI
EcoRII EcoT14I EcoT22I FbaI Fnu4HI
FokI HaeIII HapII HgaI HincII
HindII HindIII HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MamI MboI MboII MfeI MflI
MnlI MseI MslI MspA1I MspI
MunI MvaI MwoI NciI NdeI
NdeII NlaIII NlaIV NsiI NspI
PflMI PleI PstI PvuII RcaI
RsaI Sau3AI Sau96I ScaI ScrFI
SfaNI SfcI SmlI SspI StuI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I Van91I
XcmI XhoII XmnI