##################################
# NO CUT ENZYMES ( 60 enzymes) #
##################################
AccII AclI AgeI Alw44I Aor51HI
ApaLI AscI AspI BbeI BglI
BsiEI BsiWI BsmBI BspDI BsrBI
BssHII BssSI BstUI BstXI ClaI
CpoI DrdI EagI EclXI Eco47III
Eco52I FseI KasI KspI MluI
MvnI NarI NheI NotI NruI
PacI PaeR7I PflFI PflMI PinAI
PmeI Psp1406I PvuI RsrII SacII
SalI SbfI SfiI SfoI SgrAI
SmaI SnaBI SpeI Sse8387I SwaI
Tth111I Van91I XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 44 enzymes) #
###################################
AatII 3508 GACGTC GACGT^C
Acc65I 8362 GGTACC G^GTACC
AhdI 3039 GACATGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 140 CCTGAGG CC^T[ACGT]AGG
ApaI 2253 GGGCCC GGGCC^C
Asp718I 8362 GGTACC G^GTACC
AspEI 3039 GACATGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaI 6539 CTCGGG C^[CT]CG[GA]G
AviII 4155 TGCGCA TGC^GCA
AvrII 3263 CCTAGG C^CTAGG
BbrPI 3313 CACGTG CAC^GTG
BciVI 2768 GGATAC GTATCC(6/5)
BlnI 3263 CCTAGG C^CTAGG
BlpI 1687 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 1687 GCTCAGC GC^T[ACGT]AGC
BsaAI 3313 CACGTG [CT]AC^GT[GA]
BsoBI 6539 CTCGGG C^[CT]CG[GA]G
BspLU11I 10218 ACATGT A^CATGT
Bst1107I 2918 GTATAC GTA^TAC
BstAPI 608 GCAAGGTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 783 TTCGAA TT^CGAA
BstEII 6482 GGTCACC G^GT[ACGT]ACC
BstPI 6482 GGTCACC G^GT[ACGT]ACC
BstZ17I 2918 GTATAC GTA^TAC
Bsu36I 140 CCTGAGG CC^T[ACGT]AGG
CelII 1687 GCTCAGC GC^T[ACGT]AGC
Eam1105I 3039 GACATGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 140 CCTGAGG CC^T[ACGT]AGG
EcoO65I 6482 GGTCACC G^GT[ACGT]ACC
EcoRV 5076 GATATC GAT^ATC
FspI 4155 TGCGCA TGC^GCA
HaeII 7640 AGCGCC [GA]GCGC^[CT]
KpnI 8362 GGTACC GGTAC^C
MfeI 1809 CAATTG C^AATTG
MunI 1809 CAATTG C^AATTG
NaeI 688 GCCGGC GCC^GGC
NgoMIV 688 GCCGGC G^CCGGC
NspV 783 TTCGAA TT^CGAA
PmaCI 3313 CACGTG CAC^GTG
PmlI 3313 CACGTG CAC^GTG
PshAI 795 GACTACAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
ScaI 7482 AGTACT AGT^ACT
SfuI 783 TTCGAA TT^CGAA
SphI 4765 GCATGC GCATG^C
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AccIII 340 TCCGGA T^CCGGA
6876 TCCGGA
AcyI 621 GACGCC G[GA]^CG[CT]C
3508 GACGTC
AseI 3231 ATTAAT AT^TAAT
8337 ATTAAT
AsnI 3231 ATTAAT AT^TAAT
8337 ATTAAT
BalI 187 TGGCCA TGG^CCA
5117 TGGCCA
BamHI 158 GGATCC G^GATCC
4595 GGATCC
BcnI 3560 CCGGG CC^[GC]GG
5243 CCCGG
BsaBI 411 GATGTACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
9230 GATGCTTATC
BsaHI 621 GACGCC G[GA]^CG[CT]C
3508 GACGTC
BseAI 340 TCCGGA T^CCGGA
6876 TCCGGA
BspEI 340 TCCGGA T^CCGGA
6876 TCCGGA
BspMI 3319 ACCTGC ACCTGC(4/8)
5371 ACCTGC
BsrFI 688 GCCGGC [GA]^CCGG[CT]
6406 ACCGGC
Cfr10I 688 GCCGGC [GA]^CCGG[CT]
6406 ACCGGC
DraIII 543 CACGCAGTG CAC[ACGT][ACGT][ACGT]^GTG
1488 CACAAGGTG
EaeI 187 TGGCCA [CT]^GGCC[GA]
5117 TGGCCA
EcoT22I 5254 ATGCAT ATGCA^T
7059 ATGCAT
HgaI 621 GACGC GACGC(5/10)
5270 GCGTC
Hin1I 621 GACGCC G[GA]^CG[CT]C
3508 GACGTC
MamI 411 GATGTACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
9230 GATGCTTATC
MluNI 187 TGGCCA TGG^CCA
5117 TGGCCA
MroI 340 TCCGGA T^CCGGA
6876 TCCGGA
MscI 187 TGGCCA TGG^CCA
5117 TGGCCA
NciI 3560 CCGGG CC^[GC]GG
5243 CCCGG
NsiI 5254 ATGCAT ATGCA^T
7059 ATGCAT
PshBI 3231 ATTAAT AT^TAAT
8337 ATTAAT
SapI 1056 GAAGAGC GCTCTTC(1/4)
5406 GAAGAGC
StuI 1975 AGGCCT AGG^CCT
6580 AGGCCT
#########################################
# 3 OR MORE CUT ENZYMES ( 126 enzymes) #
#########################################
AccI AciI AcsI AfaI AflII
AflIII AluI AlwI AlwNI ApoI
Asp700I AspHI AvaII BanI BanII
BbsI BbvCI BbvI BcgI BclI
BfaI BfrI BglII BmyI BpmI
BpuAI BsaI BsaJI BsaWI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI BsmI Bsp1286I Bsp1407I BspHI
BsrDI BsrGI BsrI BssKI BstF5I
BstNI BstYI Cac8I CfoI Cfr13I
DdeI DpnII DraI DraII DsaI
EarI EcoNI EcoO109I EcoRI EcoRII
EcoT14I FauI FbaI Fnu4HI FokI
HaeIII HapII HhaI HinP1I HincII
HindII HindIII HinfI HpaI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MnlI
MseI MslI MspA1I MspI MvaI
MwoI NcoI NdeI NdeII NlaIII
NlaIV NspI PleI PpuMI PstI
PvuII RcaI RsaI SacI Sau3AI
Sau96I ScrFI SexAI SfaNI SfcI
SmlI SspBI SspI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I XbaI XcmI XhoII
XmnI