##################################
# NO CUT ENZYMES ( 50 enzymes) #
##################################
AccIII AflII AgeI AscI AseI
AsnI BciVI BfrI BseAI BsiWI
BspDI BspEI BspHI BspLU11I Bst1107I
BstBI BstZ17I ClaI CpoI DraI
EcoT22I HpaI MfeI MluI MroI
MseI MunI NdeI NheI NotI
NsiI NspV PacI PinAI PmeI
PshBI PvuI RcaI RsrII SalI
SbfI SfuI SgrAI SnaBI SpeI
SphI Sse8387I SspI SwaI Tru9I
###################################
# ONE CUT ENZYMES ( 37 enzymes) #
###################################
AclI 2559 AACGTT AA^CGTT
AcsI 3764 GAATTC [GA]^AATT[CT]
AflIII 3144 ACGTGT A^C[GA][CT]GT
AhdI 4598 GACCTGACGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Aor51HI 3414 AGCGCT AGC^GCT
ApoI 3764 GAATTC [GA]^AATT[CT]
AspEI 4598 GACCTGACGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII 4179 TGCGCA TGC^GCA
AvrII 5880 CCTAGG C^CTAGG
BbeI 4942 GGCGCC GGCGC^C
BbvCI 5610 GCTGAGG CCTCAGC(-5/-2)
BclI 3900 TGATCA T^GATCA
BlnI 5880 CCTAGG C^CTAGG
BsaBI 4222 GATGTCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsmI 163 GAATGC GAATGC(1/-1)
Bsp1407I 4272 TGTACA T^GTACA
BsrGI 4272 TGTACA T^GTACA
BssHII 376 GCGCGC G^CGCGC
Eam1105I 4598 GACCTGACGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 3414 AGCGCT AGC^GCT
EcoRI 3764 GAATTC G^AATTC
EcoRV 1052 GATATC GAT^ATC
FbaI 3900 TGATCA T^GATCA
FseI 2530 GGCCGGCC GGCCGG^CC
FspI 4179 TGCGCA TGC^GCA
HindIII 2986 AAGCTT A^AGCTT
KasI 4942 GGCGCC G^GCGCC
MamI 4222 GATGTCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NarI 4942 GGCGCC GG^CGCC
NruI 3547 TCGCGA TCG^CGA
PaeR7I 5791 CTCGAG C^TCGAG
Psp1406I 2559 AACGTT AA^CGTT
SfiI 1519 GGCCGAGGAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 4942 GGCGCC GGC^GCC
SspBI 4272 TGTACA T^GTACA
XbaI 5679 TCTAGA T^CTAGA
XhoI 5791 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AatII 2636 GACGTC GACGT^C
4603 GACGTC
Acc65I 5559 GGTACC G^GTACC
5568 GGTACC
AccI 3659 GTCTAC GT^[AC][GT]AC
4052 GTCTAC
Asp700I 430 GAAGTCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3760 GAAGGAATTC
Asp718I 5559 GGTACC G^GTACC
5568 GGTACC
AspI 290 GACAATGTC GAC[ACGT]^[ACGT][ACGT]GTC
3854 GACCGCGTC
BbrPI 1775 CACGTG CAC^GTG
2111 CACGTG
BcgI 3375 CGATGACGCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
2247 GCAAGAATATCG
BglII 4866 AGATCT A^GATCT
5241 AGATCT
BlpI 3601 GCTCAGC GC^T[ACGT]AGC
5197 GCTGAGC
Bpu1102I 3601 GCTCAGC GC^T[ACGT]AGC
5197 GCTGAGC
BsaWI 576 ACCGGA [AT]^CCGG[AT]
5067 TCCGGT
CelII 3601 GCTCAGC GC^T[ACGT]AGC
5197 GCTGAGC
KpnI 5559 GGTACC GGTAC^C
5568 GGTACC
NcoI 3054 CCATGG C^CATGG
3633 CCATGG
NspI 753 GCATGT [GA]CATG^[CT]
5116 GCATGT
PflFI 290 GACAATGTC GAC[ACGT]^[ACGT][ACGT]GTC
3854 GACCGCGTC
PmaCI 1775 CACGTG CAC^GTG
2111 CACGTG
PmlI 1775 CACGTG CAC^GTG
2111 CACGTG
PshAI 2000 GACAGCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
6079 GACGTAGGTC
SacI 481 GAGCTC GAGCT^C
4133 GAGCTC
Tth111I 290 GACAATGTC GAC[ACGT]^[ACGT][ACGT]GTC
3854 GACCGCGTC
XmnI 430 GAAGTCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3760 GAAGGAATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 148 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
Alw44I AlwI AlwNI AocI ApaI
ApaLI AspHI AvaI AvaII BalI
BamHI BanI BanII BbsI BbvI
BcnI BfaI BglI BmyI BpmI
BpuAI BsaAI BsaHI BsaI BsaJI
BseRI BsgI BsiEI BsiHKAI BsiYI
BslI BsmAI BsmBI BsmFI BsoBI
Bsp1286I BspMI BsrBI BsrDI BsrFI
BsrI BssKI BssSI BstAPI BstEII
BstF5I BstNI BstPI BstUI BstXI
BstYI Bsu36I Cac8I CfoI Cfr10I
Cfr13I DdeI DpnII DraII DraIII
DrdI DsaI EaeI EagI EarI
EclXI Eco52I Eco81I EcoNI EcoO109I
EcoO65I EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI Hin1I HinP1I HincII HindII
HinfI HpaII HphI ItaI Ksp632I
KspI MaeI MaeII MaeIII MboI
MboII MflI MluNI MnlI MscI
MslI MspA1I MspI MvaI MvnI
MwoI NaeI NciI NdeII NgoMIV
NlaIII NlaIV PflMI PleI PpuMI
PstI PvuII RsaI SacII SapI
Sau3AI Sau96I ScaI ScrFI SexAI
SfaNI SfcI SmaI SmlI StuI
StyI TaqI TfiI TseI Tsp45I
Tsp509I TspRI TthHB8I Van91I XcmI
XhoII XmaCI XmaI