##################################
# NO CUT ENZYMES ( 69 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AflII AgeI AhdI Aor51HI AscI
Asp718I AspEI BcgI BciVI BclI
BfrI BsaBI BseAI BsiEI BsiWI
BspDI BspEI BssHII Bst1107I BstBI
BstEII BstPI BstZ17I ClaI CpoI
EagI Eam1105I EclXI Eco47III Eco52I
EcoO65I EcoRV FbaI FseI HpaI
KpnI KspI MamI MluI MroI
NotI NruI NspV PacI PinAI
PmeI Psp1406I PvuI RsrII SacII
SalI SbfI SexAI SfiI SfuI
SgrAI SmaI SnaBI SpeI Sse8387I
XbaI XcmI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 35 enzymes) #
###################################
Alw44I 2884 GTGCAC G^TGCAC
ApaLI 2884 GTGCAC G^TGCAC
AseI 3595 ATTAAT AT^TAAT
AsnI 3595 ATTAAT AT^TAAT
AviII 866 TGCGCA TGC^GCA
BbeI 1592 GGCGCC GGCGC^C
BbvCI 3234 GCTGAGG CCTCAGC(-5/-2)
BglII 3223 AGATCT A^GATCT
BsmBI 1892 CGTCTC CGTCTC(1/5)
Bsp1407I 5374 TGTACA T^GTACA
BspLU11I 2836 ACATGT A^CATGT
BsrGI 5374 TGTACA T^GTACA
BssSI 2143 CTCGTG CACGAG(-5/-1)
BstAPI 2886 GCACAGGATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
DraIII 3297 CACACTGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 1285 GACATTATTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI 1296 CCTAAGTGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT22I 2248 ATGCAT ATGCA^T
FspI 866 TGCGCA TGC^GCA
KasI 1592 GGCGCC G^GCGCC
MfeI 2374 CAATTG C^AATTG
MunI 2374 CAATTG C^AATTG
NarI 1592 GGCGCC GG^CGCC
NheI 789 GCTAGC G^CTAGC
NsiI 2248 ATGCAT ATGCA^T
PaeR7I 1223 CTCGAG C^TCGAG
PflMI 690 CCACAGCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshAI 4463 GACCTCAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 3595 ATTAAT AT^TAAT
SfoI 1592 GGCGCC GGC^GCC
SspBI 5374 TGTACA T^GTACA
StuI 2514 AGGCCT AGG^CCT
SwaI 2447 ATTTAAAT ATTT^AAAT
Van91I 690 CCACAGCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI 1223 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AccII 117 CGCG CG^CG
583 CGCG
AflIII 552 ACGTGT A^C[GA][CT]GT
2836 ACATGT
AocI 4597 CCTCAGG CC^T[ACGT]AGG
4926 CCTTAGG
ApaI 411 GGGCCC GGGCC^C
4700 GGGCCC
AspI 273 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
1648 GACCCTGTC
AvrII 3710 CCTAGG C^CTAGG
5262 CCTAGG
BbrPI 551 CACGTG CAC^GTG
4729 CACGTG
BglI 3138 GCCACTTTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
4693 GCCCCTTGGGC
BlnI 3710 CCTAGG C^CTAGG
5262 CCTAGG
BsaWI 599 ACCGGA [AT]^CCGG[AT]
4655 ACCGGA
BstUI 117 CGCG CG^CG
583 CGCG
BstXI 3493 CCATGGCACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
5190 CCAGGTACTTGG
Bsu36I 4597 CCTCAGG CC^T[ACGT]AGG
4926 CCTTAGG
Eco81I 4597 CCTCAGG CC^T[ACGT]AGG
4926 CCTTAGG
EcoRI 2281 GAATTC G^AATTC
4769 GAATTC
MvnI 117 CGCG CG^CG
583 CGCG
NaeI 1442 GCCGGC GCC^GGC
3779 GCCGGC
NgoMIV 1442 GCCGGC G^CCGGC
3779 GCCGGC
PflFI 273 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
1648 GACCCTGTC
PmaCI 551 CACGTG CAC^GTG
4729 CACGTG
PmlI 551 CACGTG CAC^GTG
4729 CACGTG
SapI 2043 GCTCTTC GCTCTTC(1/4)
3165 GCTCTTC
ScaI 1144 AGTACT AGT^ACT
2258 AGTACT
Tth111I 273 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
1648 GACCCTGTC
#########################################
# 3 OR MORE CUT ENZYMES ( 130 enzymes) #
#########################################
AciI AcsI AcyI AfaI AluI
AlwI AlwNI ApoI Asp700I AspHI
AvaI AvaII BalI BamHI BanI
BanII BbsI BbvI BcnI BfaI
BlpI BmyI BpmI Bpu1102I BpuAI
BsaAI BsaHI BsaI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI BsmI BsoBI Bsp1286I BspHI
BspMI BsrBI BsrDI BsrFI BsrI
BssKI BstF5I BstNI BstYI Cac8I
CelII CfoI Cfr10I Cfr13I DdeI
DpnII DraI DraII DsaI EaeI
EarI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI Hin1I HinP1I HincII
HindII HindIII HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MluNI MnlI
MscI MseI MslI MspA1I MspI
MvaI MwoI NciI NcoI NdeI
NdeII NlaIII NlaIV NspI PleI
PpuMI PstI PvuII RcaI RsaI
SacI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI SphI SspI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I XhoII XmnI