##################################
# NO CUT ENZYMES ( 51 enzymes) #
##################################
Acc65I AgeI AscI AseI AsnI
Asp718I AviII AvrII BamHI BbrPI
BbvCI BciVI BlnI BsiWI BspDI
BssHII BstBI ClaI CpoI DraIII
EcoRV FseI FspI HincII HindII
HpaI KpnI KspI MfeI MunI
NaeI NgoMIV NheI NotI NruI
NspV PacI PinAI PmaCI PmeI
PmlI PshBI RsrII SacII SalI
SexAI SfuI SgrAI SnaBI SphI
XcmI
###################################
# ONE CUT ENZYMES ( 48 enzymes) #
###################################
AatII 5340 GACGTC GACGT^C
AccIII 5109 TCCGGA T^CCGGA
AclI 1731 AACGTT AA^CGTT
AhdI 864 GACAACGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 4916 CCTGAGG CC^T[ACGT]AGG
Asp700I 5092 GAAATGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 864 GACAACGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 1452 GACATCGTC GAC[ACGT]^[ACGT][ACGT]GTC
BbeI 2319 GGCGCC GGCGC^C
BcgI 998 CGACAACAGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 3971 TGATCA T^GATCA
BsaAI 3341 CACGTA [CT]AC^GT[GA]
BsaBI 598 GATGCAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseAI 5109 TCCGGA T^CCGGA
BsmBI 2383 GAGACG CGTCTC(1/5)
Bsp1407I 5652 TGTACA T^GTACA
BspEI 5109 TCCGGA T^CCGGA
BspLU11I 3699 ACATGT A^CATGT
BsrFI 1737 ACCGGC [GA]^CCGG[CT]
BsrGI 5652 TGTACA T^GTACA
Bsu36I 4916 CCTGAGG CC^T[ACGT]AGG
Cfr10I 1737 ACCGGC [GA]^CCGG[CT]
DrdI 3222 GACGTAGAAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 1804 CGGCCG C^GGCCG
Eam1105I 864 GACAACGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 1804 CGGCCG C^GGCCG
Eco52I 1804 CGGCCG C^GGCCG
Eco81I 4916 CCTGAGG CC^T[ACGT]AGG
FbaI 3971 TGATCA T^GATCA
KasI 2319 GGCGCC G^GCGCC
MamI 598 GATGCAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluI 3913 ACGCGT A^CGCGT
MroI 5109 TCCGGA T^CCGGA
NarI 2319 GGCGCC GG^CGCC
PflFI 1452 GACATCGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshAI 5500 GACTCTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Psp1406I 1731 AACGTT AA^CGTT
PvuI 502 CGATCG CGAT^CG
SapI 2700 GAAGAGC GCTCTTC(1/4)
SbfI 1611 CCTGCAGG CCTGCA^GG
SfiI 2180 GGCCCGGGAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 2319 GGCGCC GGC^GCC
SpeI 5676 ACTAGT A^CTAGT
Sse8387I 1611 CCTGCAGG CCTGCA^GG
SspBI 5652 TGTACA T^GTACA
Tth111I 1452 GACATCGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 6297 TCTAGA T^CTAGA
XmnI 5092 GAAATGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
ApaI 19 GGGCCC GGGCC^C
2093 GGGCCC
BglII 603 AGATCT A^GATCT
1375 AGATCT
BlpI 732 GCTGAGC GC^T[ACGT]AGC
4190 GCTTAGC
Bpu1102I 732 GCTGAGC GC^T[ACGT]AGC
4190 GCTTAGC
BsaWI 585 TCCGGT [AT]^CCGG[AT]
5109 TCCGGA
BspHI 439 TCATGA T^CATGA
5161 TCATGA
BssSI 5254 CACGAG CACGAG(-5/-1)
78 CTCGTG
BstEII 1658 GGTGACC G^GT[ACGT]ACC
4974 GGTCACC
BstPI 1658 GGTGACC G^GT[ACGT]ACC
4974 GGTCACC
CelII 732 GCTGAGC GC^T[ACGT]AGC
4190 GCTTAGC
EcoO65I 1658 GGTGACC G^GT[ACGT]ACC
4974 GGTCACC
EcoRI 632 GAATTC G^AATTC
4855 GAATTC
EcoT22I 5482 ATGCAT ATGCA^T
5923 ATGCAT
NcoI 2176 CCATGG C^CATGG
2378 CCATGG
NdeI 162 CATATG CA^TATG
5842 CATATG
NsiI 5482 ATGCAT ATGCA^T
5923 ATGCAT
PaeR7I 3806 CTCGAG C^TCGAG
4689 CTCGAG
PflMI 212 CCAGGCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3795 CCAAACACTGG
PvuII 192 CAGCTG CAG^CTG
2469 CAGCTG
RcaI 439 TCATGA T^CATGA
5161 TCATGA
ScaI 5171 AGTACT AGT^ACT
5782 AGTACT
SmaI 328 CCCGGG CCC^GGG
2182 CCCGGG
SwaI 4283 ATTTAAAT ATTT^AAAT
5952 ATTTAAAT
Van91I 212 CCAGGCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3795 CCAAACACTGG
XhoI 3806 CTCGAG C^TCGAG
4689 CTCGAG
XmaCI 328 CCCGGG C^CCGGG
2182 CCCGGG
XmaI 328 CCCGGG C^CCGGG
2182 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 132 enzymes) #
#########################################
AccI AccII AciI AcsI AcyI
AfaI AflII AflIII AluI Alw44I
AlwI AlwNI Aor51HI ApaLI ApoI
AspHI AvaI AvaII BalI BanI
BanII BbsI BbvI BcnI BfaI
BfrI BglI BmyI BpmI BpuAI
BsaHI BsaI BsaJI BseRI BsgI
BsiEI BsiHKAI BsiYI BslI BsmAI
BsmFI BsmI BsoBI Bsp1286I BspMI
BsrBI BsrDI BsrI BssKI Bst1107I
BstAPI BstF5I BstNI BstUI BstXI
BstYI BstZ17I Cac8I CfoI Cfr13I
DdeI DpnII DraI DraII DsaI
EaeI EarI Eco47III EcoNI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
Hin1I HinP1I HindIII HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MluNI
MnlI MscI MseI MslI MspA1I
MspI MvaI MvnI MwoI NciI
NdeII NlaIII NlaIV NspI PleI
PpuMI PstI RsaI SacI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
SspI StuI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I XhoII