##################################
# NO CUT ENZYMES ( 50 enzymes) #
##################################
AccIII AclI AflII AscI Asp700I
AspI AviII BbrPI BbvCI BfrI
BglII BseAI BsiWI BspEI BspHI
Bst1107I BstBI BstZ17I CpoI EcoRI
FspI HpaI MluI MroI NheI
NotI NruI NspV PacI PaeR7I
PflFI PmaCI PmeI PmlI PshAI
Psp1406I PvuI RcaI RsrII SalI
SapI SfuI SgrAI SnaBI SpeI
SwaI Tth111I XbaI XhoI XmnI
###################################
# ONE CUT ENZYMES ( 52 enzymes) #
###################################
AatII 1858 GACGTC GACGT^C
Acc65I 4995 GGTACC G^GTACC
AgeI 3873 ACCGGT A^CCGGT
Aor51HI 3294 AGCGCT AGC^GCT
AseI 4553 ATTAAT AT^TAAT
AsnI 4553 ATTAAT AT^TAAT
Asp718I 4995 GGTACC G^GTACC
AvrII 2945 CCTAGG C^CTAGG
BbeI 514 GGCGCC GGCGC^C
BcgI 205 CGAGGATTTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 6031 TGATCA T^GATCA
BglI 70 GCCTGGCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlnI 2945 CCTAGG C^CTAGG
BsaAI 3860 CACGTA [CT]AC^GT[GA]
BsaI 2558 GGTCTC GGTCTC(1/5)
BsaWI 3873 ACCGGT [AT]^CCGG[AT]
BsiEI 684 CGGCCG CG[GA][CT]^CG
BsmBI 1133 GAGACG CGTCTC(1/5)
BspDI 2810 ATCGAT AT^CGAT
BsrBI 89 GAGCGG CCGCTC(-3/-3)
BssSI 6082 CACGAG CACGAG(-5/-1)
BstEII 5409 GGTGACC G^GT[ACGT]ACC
BstPI 5409 GGTGACC G^GT[ACGT]ACC
ClaI 2810 ATCGAT AT^CGAT
EagI 684 CGGCCG C^GGCCG
EclXI 684 CGGCCG C^GGCCG
Eco47III 3294 AGCGCT AGC^GCT
Eco52I 684 CGGCCG C^GGCCG
EcoNI 5576 CCTTGAAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 5409 GGTGACC G^GT[ACGT]ACC
EcoRV 917 GATATC GAT^ATC
EcoT22I 5067 ATGCAT ATGCA^T
FbaI 6031 TGATCA T^GATCA
FseI 2395 GGCCGGCC GGCCGG^CC
KasI 514 GGCGCC G^GCGCC
KpnI 4995 GGTACC GGTAC^C
KspI 5 CCGCGG CCGC^GG
NaeI 2396 GCCGGC GCC^GGC
NarI 514 GGCGCC GG^CGCC
NgoMIV 2396 GCCGGC G^CCGGC
NsiI 5067 ATGCAT ATGCA^T
PinAI 3873 ACCGGT A^CCGGT
PshBI 4553 ATTAAT AT^TAAT
SacII 5 CCGCGG CCGC^GG
SbfI 2974 CCTGCAGG CCTGCA^GG
ScaI 4837 AGTACT AGT^ACT
SexAI 744 ACCTGGT A^CC[AT]GGT
SfiI 69 GGCCTGGCTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 514 GGCGCC GGC^GCC
SphI 5432 GCATGC GCATG^C
Sse8387I 2974 CCTGCAGG CCTGCA^GG
SspI 2528 AATATT AAT^ATT
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AhdI 1713 GACTTTATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4147 GACCTGCAGTC
Alw44I 505 GTGCAC G^TGCAC
6659 GTGCAC
AocI 911 CCTGAGG CC^T[ACGT]AGG
4918 CCTGAGG
ApaLI 505 GTGCAC G^TGCAC
6659 GTGCAC
AspEI 1713 GACTTTATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4147 GACCTGCAGTC
BciVI 3593 GTATCC GTATCC(6/5)
3853 GTATCC
BlpI 1354 GCTCAGC GC^T[ACGT]AGC
6291 GCTAAGC
Bpu1102I 1354 GCTCAGC GC^T[ACGT]AGC
6291 GCTAAGC
BsaBI 3848 GATGTGTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
4024 GATTTCTATC
BsrFI 2396 GCCGGC [GA]^CCGG[CT]
3873 ACCGGT
BssHII 248 GCGCGC G^CGCGC
250 GCGCGC
BstAPI 563 GCAGGTTGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
3441 GCATCTGCTGC
Bsu36I 911 CCTGAGG CC^T[ACGT]AGG
4918 CCTGAGG
CelII 1354 GCTCAGC GC^T[ACGT]AGC
6291 GCTAAGC
Cfr10I 2396 GCCGGC [GA]^CCGG[CT]
3873 ACCGGT
DraIII 496 CACTGTGTG CAC[ACGT][ACGT][ACGT]^GTG
2825 CACAGTGTG
DrdI 1964 GACTCTCCCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3062 GACATTAAGGTC
Eam1105I 1713 GACTTTATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4147 GACCTGCAGTC
Eco81I 911 CCTGAGG CC^T[ACGT]AGG
4918 CCTGAGG
HgaI 1135 GACGC GACGC(5/10)
2254 GACGC
HindIII 446 AAGCTT A^AGCTT
4234 AAGCTT
MamI 3848 GATGTGTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
4024 GATTTCTATC
NdeI 4748 CATATG CA^TATG
5537 CATATG
StuI 836 AGGCCT AGG^CCT
1575 AGGCCT
TaqI 729 TCGA T^CGA
2811 TCGA
TthHB8I 729 TCGA T^CGA
2811 TCGA
XcmI 705 CCAGCTGTGACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
2625 CCACCCTCACTGTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 129 enzymes) #
#########################################
AccI AccII AciI AcsI AcyI
AfaI AflIII AluI AlwI AlwNI
ApaI ApoI AspHI AvaI AvaII
BalI BamHI BanI BanII BbsI
BbvI BcnI BfaI BmyI BpmI
BpuAI BsaHI BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsmI BsoBI Bsp1286I Bsp1407I BspLU11I
BspMI BsrDI BsrGI BsrI BssKI
BstF5I BstNI BstUI BstXI BstYI
Cac8I CfoI Cfr13I DdeI DpnII
DraI DraII DsaI EaeI EarI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HhaI
Hin1I HinP1I HincII HindII HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MfeI
MflI MluNI MnlI MscI MseI
MslI MspA1I MspI MunI MvaI
MvnI MwoI NciI NcoI NdeII
NlaIII NlaIV NspI PflMI PleI
PpuMI PstI PvuII RsaI SacI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmaI SmlI SspBI StyI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
Van91I XhoII XmaCI XmaI