##################################
# NO CUT ENZYMES ( 59 enzymes) #
##################################
Acc65I AccIII AclI AocI Aor51HI
AscI AseI AsnI Asp718I AviII
AvrII BbeI BbrPI BciVI BlnI
BsaAI BseAI BsiWI BspEI BspHI
BspLU11I BssHII Bst1107I BstBI BstZ17I
Bsu36I CpoI DraI Eco47III Eco81I
EcoRV FseI FspI HpaI KasI
KpnI MluI MroI NarI NdeI
NheI NruI NspV PacI PmaCI
PmeI PmlI PshBI Psp1406I PvuI
RcaI RsrII ScaI SfoI SfuI
SnaBI SpeI SwaI XbaI
###################################
# ONE CUT ENZYMES ( 41 enzymes) #
###################################
AatII 6195 GACGTC GACGT^C
AflII 1358 CTTAAG C^TTAAG
AflIII 2682 ACACGT A^C[GA][CT]GT
AgeI 3186 ACCGGT A^CCGGT
AhdI 5386 GACCAGCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
ApaI 3981 GGGCCC GGGCC^C
Asp700I 1876 GAAGCCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 5386 GACCAGCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BamHI 5065 GGATCC G^GATCC
BcgI 5125 CGAAGCTGCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfaI 1542 CTAG C^TAG
BfrI 1358 CTTAAG C^TTAAG
BglII 5208 AGATCT A^GATCT
BsaBI 1321 GATGGAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
Bsp1407I 6400 TGTACA T^GTACA
BsrBI 415 CCGCTC CCGCTC(-3/-3)
BsrGI 6400 TGTACA T^GTACA
BstEII 4540 GGTGACC G^GT[ACGT]ACC
BstPI 4540 GGTGACC G^GT[ACGT]ACC
Eam1105I 5386 GACCAGCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 1819 GAAGAG CTCTTC(1/4)
EcoNI 472 CCTGAAGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 4540 GGTGACC G^GT[ACGT]ACC
HindIII 1308 AAGCTT A^AGCTT
Ksp632I 1819 GAAGAG CTCTTC(1/4)
KspI 1614 CCGCGG CCGC^GG
MaeI 1542 CTAG C^TAG
MamI 1321 GATGGAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NaeI 5006 GCCGGC GCC^GGC
NgoMIV 5006 GCCGGC G^CCGGC
NotI 1616 GCGGCCGC GC^GGCCGC
PinAI 3186 ACCGGT A^CCGGT
SacII 1614 CCGCGG CCGC^GG
SalI 3728 GTCGAC G^TCGAC
SapI 1819 GAAGAGC GCTCTTC(1/4)
SbfI 3271 CCTGCAGG CCTGCA^GG
SgrAI 5005 CGCCGGCG C[GA]^CCGG[CT]G
Sse8387I 3271 CCTGCAGG CCTGCA^GG
SspBI 6400 TGTACA T^GTACA
SspI 6205 AATATT AAT^ATT
XmnI 1876 GAAGCCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AccI 3728 GTCGAC GT^[AC][GT]AC
4764 GTAGAC
AccII 660 CGCG CG^CG
1615 CGCG
Alw44I 4903 GTGCAC G^TGCAC
6058 GTGCAC
ApaLI 4903 GTGCAC G^TGCAC
6058 GTGCAC
BanI 3858 GGCACC G^G[CT][GA]CC
4601 GGCACC
BsaWI 2426 ACCGGA [AT]^CCGG[AT]
3186 ACCGGT
BsiEI 568 CGGCCG CG[GA][CT]^CG
1617 CGGCCG
BspMI 478 GCAGGT ACCTGC(4/8)
5464 GCAGGT
BsrFI 3186 ACCGGT [GA]^CCGG[CT]
5006 GCCGGC
BssSI 3244 CACGAG CACGAG(-5/-1)
3930 CTCGTG
BstUI 660 CGCG CG^CG
1615 CGCG
Cfr10I 3186 ACCGGT [GA]^CCGG[CT]
5006 GCCGGC
DraIII 301 CACCCAGTG CAC[ACGT][ACGT][ACGT]^GTG
2230 CACTCTGTG
DrdI 5075 GACCCAACTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
5579 GACAATCTTGTC
EagI 568 CGGCCG C^GGCCG
1617 CGGCCG
EclXI 568 CGGCCG C^GGCCG
1617 CGGCCG
Eco52I 568 CGGCCG C^GGCCG
1617 CGGCCG
EcoT22I 4108 ATGCAT ATGCA^T
4630 ATGCAT
MfeI 2667 CAATTG C^AATTG
4623 CAATTG
MunI 2667 CAATTG C^AATTG
4623 CAATTG
MvnI 660 CGCG CG^CG
1615 CGCG
NsiI 4108 ATGCAT ATGCA^T
4630 ATGCAT
PaeR7I 1644 CTCGAG C^TCGAG
2288 CTCGAG
SexAI 2793 ACCTGGT A^CC[AT]GGT
3065 ACCTGGT
SphI 2609 GCATGC GCATG^C
4106 GCATGC
XhoI 1644 CTCGAG C^TCGAG
2288 CTCGAG
#########################################
# 3 OR MORE CUT ENZYMES ( 132 enzymes) #
#########################################
AciI AcsI AcyI AfaI AluI
AlwI AlwNI ApoI AspHI AspI
AvaI AvaII BalI BanII BbsI
BbvCI BbvI BclI BcnI BglI
BlpI BmyI BpmI Bpu1102I BpuAI
BsaHI BsaI BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmBI
BsmFI BsmI BsoBI Bsp1286I BspDI
BsrDI BsrI BssKI BstAPI BstF5I
BstNI BstXI BstYI Cac8I CelII
CfoI Cfr13I ClaI DdeI DpnII
DraII DsaI EaeI EcoO109I EcoRI
EcoRII EcoT14I FauI FbaI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI Hin1I HinP1I HincII HindII
HinfI HpaII HphI ItaI MaeII
MaeIII MboI MboII MflI MluNI
MnlI MscI MseI MslI MspA1I
MspI MvaI MwoI NciI NcoI
NdeII NlaIII NlaIV NspI PflFI
PflMI PleI PpuMI PshAI PstI
PvuII RsaI SacI Sau3AI Sau96I
ScrFI SfaNI SfcI SfiI SmaI
SmlI StuI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
Tth111I TthHB8I Van91I XcmI XhoII
XmaCI XmaI