##################################
# NO CUT ENZYMES ( 69 enzymes) #
##################################
AatII AccII AccIII AclI AgeI
AhdI Aor51HI AscI AseI AsnI
AspEI AviII BbeI BbvCI BseAI
BsiEI BsiWI BsmBI Bsp1407I BspDI
BspEI BsrGI BssHII BstBI BstUI
ClaI CpoI EagI Eam1105I EclXI
Eco47III Eco52I EcoRV FseI FspI
HindIII HpaI KasI KspI MfeI
MluI MroI MunI MvnI NarI
NheI NotI NruI NspV PacI
PaeR7I PinAI PmeI PshBI Psp1406I
PvuI RsrII SacII SbfI SfiI
SfoI SfuI SgrAI SnaBI SpeI
Sse8387I SspBI SwaI XhoI
###################################
# ONE CUT ENZYMES ( 41 enzymes) #
###################################
Acc65I 2436 GGTACC G^GTACC
Alw44I 4896 GTGCAC G^TGCAC
AocI 3402 CCTGAGG CC^T[ACGT]AGG
ApaI 1292 GGGCCC GGGCC^C
ApaLI 4896 GTGCAC G^TGCAC
Asp718I 2436 GGTACC G^GTACC
AspI 5759 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
BcgI 3387 CGATTACTTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BlpI 5964 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 5964 GCTCAGC GC^T[ACGT]AGC
BsaBI 2291 GATCACCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 2439 ACCGGA [AT]^CCGG[AT]
BspLU11I 5672 ACATGT A^CATGT
BsrBI 5421 GAGCGG CCGCTC(-3/-3)
BssSI 2591 CTCGTG CACGAG(-5/-1)
Bst1107I 2339 GTATAC GTA^TAC
BstEII 2860 GGTCACC G^GT[ACGT]ACC
BstPI 2860 GGTCACC G^GT[ACGT]ACC
BstZ17I 2339 GTATAC GTA^TAC
Bsu36I 3402 CCTGAGG CC^T[ACGT]AGG
CelII 5964 GCTCAGC GC^T[ACGT]AGC
DraI 4972 TTTAAA TTT^AAA
DrdI 1507 GACACGGCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 3402 CCTGAGG CC^T[ACGT]AGG
EcoO65I 2860 GGTCACC G^GT[ACGT]ACC
EcoRI 4367 GAATTC G^AATTC
EcoT22I 1418 ATGCAT ATGCA^T
HincII 29 GTCGAC GT[CT]^[GA]AC
HindII 29 GTCGAC GT[CT]^[GA]AC
KpnI 2436 GGTACC GGTAC^C
MamI 2291 GATCACCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NcoI 4605 CCATGG C^CATGG
NdeI 1376 CATATG CA^TATG
NsiI 1418 ATGCAT ATGCA^T
PflFI 5759 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
SalI 29 GTCGAC G^TCGAC
SexAI 4030 ACCAGGT A^CC[AT]GGT
SphI 3207 GCATGC GCATG^C
SspI 3046 AATATT AAT^ATT
StuI 1109 AGGCCT AGG^CCT
Tth111I 5759 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AccI 29 GTCGAC GT^[AC][GT]AC
2339 GTATAC
AcyI 2501 GGCGTC G[GA]^CG[CT]C
6241 GGCGTC
AflII 931 CTTAAG C^TTAAG
3886 CTTAAG
AvrII 4738 CCTAGG C^CTAGG
5538 CCTAGG
BamHI 1975 GGATCC G^GATCC
5534 GGATCC
BbsI 333 GTCTTC GAAGAC(2/6)
5846 GTCTTC
BciVI 5388 GGATAC GTATCC(6/5)
3370 GTATCC
BfrI 931 CTTAAG C^TTAAG
3886 CTTAAG
BglII 3092 AGATCT A^GATCT
3908 AGATCT
BlnI 4738 CCTAGG C^CTAGG
5538 CCTAGG
BpuAI 333 GTCTTC GAAGAC(2/6)
5846 GTCTTC
BsaHI 2501 GGCGTC G[GA]^CG[CT]C
6241 GGCGTC
BsaI 2278 GAGACC GGTCTC(1/5)
3168 GGTCTC
BsmI 3493 GAATGC GAATGC(1/-1)
65 GCATTC
BsrFI 2525 GCCGGC [GA]^CCGG[CT]
2986 GCCGGC
BstXI 1917 CCACTTCTATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4709 CCAGTGGAGTGG
Cfr10I 2525 GCCGGC [GA]^CCGG[CT]
2986 GCCGGC
DraIII 382 CACCCTGTG CAC[ACGT][ACGT][ACGT]^GTG
1830 CACCATGTG
DsaI 178 CCACGG C^C[GA][CT]GG
4605 CCATGG
Hin1I 2501 GGCGTC G[GA]^CG[CT]C
6241 GGCGTC
NaeI 2525 GCCGGC GCC^GGC
2986 GCCGGC
NgoMIV 2525 GCCGGC G^CCGGC
2986 GCCGGC
PshAI 1935 GACTGCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
6099 GACATCTGTC
SapI 581 GCTCTTC GCTCTTC(1/4)
3574 GCTCTTC
ScaI 3146 AGTACT AGT^ACT
3716 AGTACT
XcmI 2549 CCACTAAGAAAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
2906 CCAACCCAGAGGTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 122 enzymes) #
#########################################
AciI AcsI AfaI AflIII AluI
AlwI AlwNI ApoI Asp700I AspHI
AvaI AvaII BalI BanI BanII
BbrPI BbvI BclI BcnI BfaI
BglI BmyI BpmI BsaAI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BspHI
BspMI BsrDI BsrI BssKI BstAPI
BstF5I BstNI BstYI Cac8I CfoI
Cfr13I DdeI DpnII DraII EaeI
EarI EcoNI EcoO109I EcoRII EcoT14I
FauI FbaI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI HinP1I
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MluNI MnlI MscI MseI
MslI MspA1I MspI MvaI MwoI
NciI NdeII NlaIII NlaIV NspI
PflMI PleI PmaCI PmlI PpuMI
PstI PvuII RcaI RsaI SacI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmaI SmlI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I Van91I XbaI XhoII XmaCI
XmaI XmnI