##################################
# NO CUT ENZYMES ( 47 enzymes) #
##################################
AatII AccIII AclI AflII AgeI
AscI AseI AsnI AviII AvrII
BamHI BfrI BlnI BsaWI BseAI
BsiWI Bsp1407I BspEI BsrGI Bst1107I
BstZ17I CpoI EagI EclXI Eco52I
FseI FspI MfeI MroI MunI
NaeI NgoMIV NotI NruI PacI
PinAI PmeI PshAI PshBI Psp1406I
RsrII SalI SfiI SnaBI SpeI
SspBI SwaI
###################################
# ONE CUT ENZYMES ( 42 enzymes) #
###################################
Acc65I 2849 GGTACC G^GTACC
AccI 5004 GTAGAC GT^[AC][GT]AC
AhdI 2880 GACCCGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
ApaI 1108 GGGCCC GGGCC^C
Asp700I 972 GAAAACTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 2849 GGTACC G^GTACC
AspEI 2880 GACCCGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BbeI 246 GGCGCC GGCGC^C
BbvCI 1851 GCTGAGG CCTCAGC(-5/-2)
BglI 663 GCCGCCATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BspHI 5994 TCATGA T^CATGA
BstBI 3910 TTCGAA TT^CGAA
DraIII 590 CACGTGGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 1198 GACAAGGAAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 2880 GACCCGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRI 4761 GAATTC G^AATTC
EcoRV 339 GATATC GAT^ATC
EcoT22I 6112 ATGCAT ATGCA^T
HincII 5384 GTTAAC GT[CT]^[GA]AC
HindII 5384 GTTAAC GT[CT]^[GA]AC
HpaI 5384 GTTAAC GTT^AAC
KasI 246 GGCGCC G^GCGCC
KpnI 2849 GGTACC GGTAC^C
MluI 2626 ACGCGT A^CGCGT
NarI 246 GGCGCC GG^CGCC
NdeI 5824 CATATG CA^TATG
NheI 5642 GCTAGC G^CTAGC
NsiI 6112 ATGCAT ATGCA^T
NspV 3910 TTCGAA TT^CGAA
PaeR7I 5009 CTCGAG C^TCGAG
PvuI 1811 CGATCG CGAT^CG
RcaI 5994 TCATGA T^CATGA
SbfI 4160 CCTGCAGG CCTGCA^GG
SfoI 246 GGCGCC GGC^GCC
SfuI 3910 TTCGAA TT^CGAA
SgrAI 702 CGCCGGTG C[GA]^CCGG[CT]G
SmaI 3667 CCCGGG CCC^GGG
Sse8387I 4160 CCTGCAGG CCTGCA^GG
XhoI 5009 CTCGAG C^TCGAG
XmaCI 3667 CCCGGG C^CCGGG
XmaI 3667 CCCGGG C^CCGGG
XmnI 972 GAAAACTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 30 enzymes) #
###################################
Alw44I 254 GTGCAC G^TGCAC
6212 GTGCAC
AocI 761 CCTCAGG CC^T[ACGT]AGG
3053 CCTGAGG
ApaLI 254 GTGCAC G^TGCAC
6212 GTGCAC
AspI 816 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
888 GACCTGGTC
BcgI 699 CGACGCCGGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
2734 GCAACAGGTTCG
BciVI 366 GGATAC GTATCC(6/5)
5351 GTATCC
BclI 1216 TGATCA T^GATCA
5896 TGATCA
BlpI 2402 GCTCAGC GC^T[ACGT]AGC
4018 GCTTAGC
Bpu1102I 2402 GCTCAGC GC^T[ACGT]AGC
4018 GCTTAGC
BspDI 1038 ATCGAT AT^CGAT
3084 ATCGAT
BsrFI 97 ACCGGC [GA]^CCGG[CT]
703 GCCGGT
BstEII 821 GGTCACC G^GT[ACGT]ACC
5115 GGTTACC
BstPI 821 GGTCACC G^GT[ACGT]ACC
5115 GGTTACC
Bsu36I 761 CCTCAGG CC^T[ACGT]AGG
3053 CCTGAGG
CelII 2402 GCTCAGC GC^T[ACGT]AGC
4018 GCTTAGC
Cfr10I 97 ACCGGC [GA]^CCGG[CT]
703 GCCGGT
ClaI 1038 ATCGAT AT^CGAT
3084 ATCGAT
Eco81I 761 CCTCAGG CC^T[ACGT]AGG
3053 CCTGAGG
EcoO65I 821 GGTCACC G^GT[ACGT]ACC
5115 GGTTACC
FbaI 1216 TGATCA T^GATCA
5896 TGATCA
HindIII 4016 AAGCTT A^AGCTT
6232 AAGCTT
KspI 2271 CCGCGG CCGC^GG
5065 CCGCGG
PflFI 816 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
888 GACCTGGTC
SacII 2271 CCGCGG CCGC^GG
5065 CCGCGG
ScaI 4552 AGTACT AGT^ACT
6628 AGTACT
SexAI 817 ACCTGGT A^CC[AT]GGT
889 ACCTGGT
SphI 2311 GCATGC GCATG^C
3208 GCATGC
SspI 4747 AATATT AAT^ATT
5656 AATATT
Tth111I 816 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
888 GACCTGGTC
XbaI 681 TCTAGA T^CTAGA
3852 TCTAGA
#########################################
# 3 OR MORE CUT ENZYMES ( 139 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AflIII AluI AlwI AlwNI Aor51HI
ApoI AspHI AvaI AvaII BalI
BanI BanII BbrPI BbsI BbvI
BcnI BfaI BglII BmyI BpmI
BpuAI BsaAI BsaBI BsaHI BsaI
BsaJI BseRI BsgI BsiEI BsiHKAI
BsiYI BslI BsmAI BsmBI BsmFI
BsmI BsoBI Bsp1286I BspLU11I BspMI
BsrBI BsrDI BsrI BssHII BssKI
BssSI BstAPI BstF5I BstNI BstUI
BstXI BstYI Cac8I CfoI Cfr13I
DdeI DpnII DraI DraII DsaI
EaeI EarI Eco47III EcoNI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
Hin1I HinP1I HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MamI MboI MboII MflI MluNI
MnlI MscI MseI MslI MspA1I
MspI MvaI MvnI MwoI NciI
NcoI NdeII NlaIII NlaIV NspI
PflMI PleI PmaCI PmlI PpuMI
PstI PvuII RsaI SacI SapI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI StuI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I Van91I XcmI XhoII