##################################
# NO CUT ENZYMES ( 62 enzymes) #
##################################
Acc65I AccI AclI AflII AgeI
AscI Asp718I AviII AvrII BbrPI
BclI BfrI BglI BlnI BsaAI
BsgI BsiEI BsiWI BspDI BsrBI
BssHII BssSI Bst1107I BstBI BstEII
BstPI BstZ17I ClaI DraIII DrdI
EagI EclXI Eco52I EcoNI EcoO65I
FbaI FseI FspI KpnI NaeI
NgoMIV NheI NotI NruI NspV
PacI PinAI PmaCI PmeI PmlI
Psp1406I PvuI SalI SbfI SexAI
SfiI SfuI SgrAI SnaBI SpeI
Sse8387I SwaI
###################################
# ONE CUT ENZYMES ( 53 enzymes) #
###################################
AatII 1811 GACGTC GACGT^C
AccIII 2139 TCCGGA T^CCGGA
AhdI 526 GACAGGAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 2665 GTGCAC G^TGCAC
Aor51HI 200 AGCGCT AGC^GCT
ApaI 4992 GGGCCC GGGCC^C
ApaLI 2665 GTGCAC G^TGCAC
AspEI 526 GACAGGAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 6464 GACTCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
BalI 1801 TGGCCA TGG^CCA
BbeI 6338 GGCGCC GGCGC^C
BcgI 5618 GCACCTCCATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 3659 GGATAC GTATCC(6/5)
BlpI 2171 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 2171 GCTCAGC GC^T[ACGT]AGC
BsaWI 2139 TCCGGA [AT]^CCGG[AT]
BseAI 2139 TCCGGA T^CCGGA
BspEI 2139 TCCGGA T^CCGGA
BsrDI 3000 CATTGC GCAATG(2/0)
BstAPI 2088 GCATATCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
CelII 2171 GCTCAGC GC^T[ACGT]AGC
CpoI 2457 CGGTCCG CG^G[AT]CCG
Eam1105I 526 GACAGGAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 200 AGCGCT AGC^GCT
EcoRI 2600 GAATTC G^AATTC
HincII 5774 GTTAAC GT[CT]^[GA]AC
HindII 5774 GTTAAC GT[CT]^[GA]AC
HindIII 2687 AAGCTT A^AGCTT
HpaI 5774 GTTAAC GTT^AAC
KasI 6338 GGCGCC G^GCGCC
KspI 474 CCGCGG CCGC^GG
MfeI 4025 CAATTG C^AATTG
MluI 352 ACGCGT A^CGCGT
MluNI 1801 TGGCCA TGG^CCA
MroI 2139 TCCGGA T^CCGGA
MscI 1801 TGGCCA TGG^CCA
MunI 4025 CAATTG C^AATTG
NarI 6338 GGCGCC GG^CGCC
NcoI 2588 CCATGG C^CATGG
PaeR7I 2061 CTCGAG C^TCGAG
PflFI 6464 GACTCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshAI 4816 GACACAAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI 6429 CTGCAG CTGCA^G
RsrII 2457 CGGTCCG CG^G[AT]CCG
SacII 474 CCGCGG CCGC^GG
SfoI 6338 GGCGCC GGC^GCC
SmaI 6390 CCCGGG CCC^GGG
SphI 1480 GCATGC GCATG^C
StuI 1598 AGGCCT AGG^CCT
Tth111I 6464 GACTCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoI 2061 CTCGAG C^TCGAG
XmaCI 6390 CCCGGG C^CCGGG
XmaI 6390 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 30 enzymes) #
###################################
AcyI 1811 GACGTC G[GA]^CG[CT]C
6338 GGCGCC
AocI 2389 CCTAAGG CC^T[ACGT]AGG
2726 CCTCAGG
AseI 4191 ATTAAT AT^TAAT
5376 ATTAAT
AsnI 4191 ATTAAT AT^TAAT
5376 ATTAAT
BamHI 922 GGATCC G^GATCC
1924 GGATCC
BanI 5617 GGCACC G^G[CT][GA]CC
6338 GGCGCC
BglII 950 AGATCT A^GATCT
4355 AGATCT
BsaBI 656 GATGAATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
4188 GATATTAATC
BsaHI 1811 GACGTC G[GA]^CG[CT]C
6338 GGCGCC
Bsp1407I 2308 TGTACA T^GTACA
5053 TGTACA
BspHI 3958 TCATGA T^CATGA
6247 TCATGA
BspMI 1714 ACCTGC ACCTGC(4/8)
3311 GCAGGT
BsrFI 181 ACCGGC [GA]^CCGG[CT]
5633 GCCGGT
BsrGI 2308 TGTACA T^GTACA
5053 TGTACA
BstXI 6217 CCAGCACTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
6351 CCAGCTACTTGG
Bsu36I 2389 CCTAAGG CC^T[ACGT]AGG
2726 CCTCAGG
Cfr10I 181 ACCGGC [GA]^CCGG[CT]
5633 GCCGGT
Eco81I 2389 CCTAAGG CC^T[ACGT]AGG
2726 CCTCAGG
EcoT22I 778 ATGCAT ATGCA^T
2997 ATGCAT
HaeII 200 AGCGCT [GA]GCGC^[CT]
6338 GGCGCC
HgaI 1061 GACGC GACGC(5/10)
1123 GCGTC
Hin1I 1811 GACGTC G[GA]^CG[CT]C
6338 GGCGCC
MamI 656 GATGAATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
4188 GATATTAATC
NdeI 1533 CATATG CA^TATG
5049 CATATG
NsiI 778 ATGCAT ATGCA^T
2997 ATGCAT
PshBI 4191 ATTAAT AT^TAAT
5376 ATTAAT
RcaI 3958 TCATGA T^CATGA
6247 TCATGA
SacI 581 GAGCTC GAGCT^C
5919 GAGCTC
SspBI 2308 TGTACA T^GTACA
5053 TGTACA
XcmI 2772 CCAGGCACACGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
4418 CCAAGAATCCACTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 113 enzymes) #
#########################################
AccII AciI AcsI AfaI AflIII
AluI AlwI AlwNI ApoI Asp700I
AspHI AvaI AvaII BanII BbsI
BbvCI BbvI BcnI BfaI BmyI
BpmI BpuAI BsaI BsaJI BseRI
BsiHKAI BsiYI BslI BsmAI BsmBI
BsmFI BsmI BsoBI Bsp1286I BspLU11I
BsrI BssKI BstF5I BstNI BstUI
BstYI Cac8I CfoI Cfr13I DdeI
DpnII DraI DraII DsaI EaeI
EarI EcoO109I EcoRII EcoRV EcoT14I
FauI Fnu4HI FokI HaeIII HapII
HhaI HinP1I HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MnlI MseI
MslI MspA1I MspI MvaI MvnI
MwoI NciI NdeII NlaIII NlaIV
NspI PflMI PleI PpuMI PvuII
RsaI SapI Sau3AI Sau96I ScaI
ScrFI SfaNI SfcI SmlI SspI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I Van91I
XbaI XhoII XmnI