##################################
# NO CUT ENZYMES ( 79 enzymes) #
##################################
AatII Acc65I AcyI AhdI AocI
Aor51HI ApaI AscI Asp718I AspEI
AvaI BbeI BbrPI BbvCI BcgI
BciVI BcnI BglI BpmI BsaHI
BsiWI BsoBI BspMI BssHII BssSI
Bst1107I BstBI BstEII BstPI BstZ17I
Bsu36I CpoI DrdI EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoNI
EcoO65I FseI Hin1I HpaI KasI
KpnI KspI MfeI MluI MunI
NaeI NarI NciI NgoMIV NheI
NotI NruI NspV PacI PaeR7I
PmaCI PmlI PshAI PvuI RsrII
SacII SalI SbfI SexAI SfiI
SfoI SfuI SmaI SnaBI Sse8387I
SwaI XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 45 enzymes) #
###################################
AccI 2820 GTAGAC GT^[AC][GT]AC
AccII 736 CGCG CG^CG
AccIII 1133 TCCGGA T^CCGGA
AclI 5753 AACGTT AA^CGTT
AgeI 3502 ACCGGT A^CCGGT
AviII 1150 TGCGCA TGC^GCA
AvrII 6591 CCTAGG C^CTAGG
BalI 3922 TGGCCA TGG^CCA
BamHI 5071 GGATCC G^GATCC
BanI 2084 GGTGCC G^G[CT][GA]CC
BlnI 6591 CCTAGG C^CTAGG
BsaI 3054 GGTCTC GGTCTC(1/5)
BseAI 1133 TCCGGA T^CCGGA
BsgI 6421 GTGCAG GTGCAG(16/14)
BsiEI 4840 CGGTCG CG[GA][CT]^CG
BsmBI 5733 CGTCTC CGTCTC(1/5)
BspDI 2159 ATCGAT AT^CGAT
BspEI 1133 TCCGGA T^CCGGA
BsrBI 1163 GAGCGG CCGCTC(-3/-3)
BsrFI 3502 ACCGGT [GA]^CCGG[CT]
BstUI 736 CGCG CG^CG
Cfr10I 3502 ACCGGT [GA]^CCGG[CT]
ClaI 2159 ATCGAT AT^CGAT
DraII 3638 GGGACCT [GA]G^G[ACGT]CC[CT]
EaeI 3922 TGGCCA [CT]^GGCC[GA]
EcoO109I 3638 GGGACCT [GA]G^G[ACGT]CC[CT]
EcoRV 3715 GATATC GAT^ATC
FspI 1150 TGCGCA TGC^GCA
HaeII 5847 GGCGCT [GA]GCGC^[CT]
MluNI 3922 TGGCCA TGG^CCA
MroI 1133 TCCGGA T^CCGGA
MscI 3922 TGGCCA TGG^CCA
MspA1I 333 CAGCTG C[AC]G^C[GT]G
MvnI 736 CGCG CG^CG
PinAI 3502 ACCGGT A^CCGGT
PmeI 4018 GTTTAAAC GTTT^AAAC
PpuMI 3638 GGGACCT [GA]G^G[AT]CC[CT]
Psp1406I 5753 AACGTT AA^CGTT
PstI 1439 CTGCAG CTGCA^G
PvuII 333 CAGCTG CAG^CTG
ScaI 2493 AGTACT AGT^ACT
SgrAI 3501 CACCGGTG C[GA]^CCGG[CT]G
SphI 4611 GCATGC GCATG^C
StuI 5151 AGGCCT AGG^CCT
XbaI 2996 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 41 enzymes) #
###################################
AflII 1598 CTTAAG C^TTAAG
3599 CTTAAG
AlwNI 333 CAGCTGCTG CAG[ACGT][ACGT][ACGT]^CTG
705 CAGATTCTG
AseI 839 ATTAAT AT^TAAT
4678 ATTAAT
AsnI 839 ATTAAT AT^TAAT
4678 ATTAAT
AspI 1228 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
2473 GACTGAGTC
BclI 2001 TGATCA T^GATCA
5727 TGATCA
BfrI 1598 CTTAAG C^TTAAG
3599 CTTAAG
BlpI 272 GCTAAGC GC^T[ACGT]AGC
4518 GCTGAGC
Bpu1102I 272 GCTAAGC GC^T[ACGT]AGC
4518 GCTGAGC
BsaAI 1314 TACGTG [CT]AC^GT[GA]
5533 TACGTG
BsaWI 1133 TCCGGA [AT]^CCGG[AT]
3502 ACCGGT
BspHI 3228 TCATGA T^CATGA
5267 TCATGA
BsrDI 4788 CATTGC GCAATG(2/0)
1673 GCAATG
BstAPI 4615 GCAAATATTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
4942 GCAAGGGATGC
CelII 272 GCTAAGC GC^T[ACGT]AGC
4518 GCTGAGC
DraIII 6271 CACACAGTG CAC[ACGT][ACGT][ACGT]^GTG
6273 CACAGTGTG
DsaI 4492 CCATGG C^C[GA][CT]GG
6144 CCATGG
EcoRI 2136 GAATTC G^AATTC
3930 GAATTC
EcoT22I 93 ATGCAT ATGCA^T
3214 ATGCAT
FauI 1636 GCGGG CCCGC(4/6)
4980 GCGGG
FbaI 2001 TGATCA T^GATCA
5727 TGATCA
HapII 1134 CCGG C^CGG
3503 CCGG
HgaI 734 GACGC GACGC(5/10)
721 GCGTC
HincII 3948 GTTGAC GT[CT]^[GA]AC
5446 GTTGAC
HindII 3948 GTTGAC GT[CT]^[GA]AC
5446 GTTGAC
HindIII 1424 AAGCTT A^AGCTT
1563 AAGCTT
HpaII 1134 CCGG C^CGG
3503 CCGG
MspI 1134 CCGG C^CGG
3503 CCGG
NcoI 4492 CCATGG C^CATGG
6144 CCATGG
NdeI 86 CATATG CA^TATG
5784 CATATG
NsiI 93 ATGCAT ATGCA^T
3214 ATGCAT
PflFI 1228 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
2473 GACTGAGTC
PflMI 4492 CCATGGTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
6731 CCACCTCATGG
PshBI 839 ATTAAT AT^TAAT
4678 ATTAAT
RcaI 3228 TCATGA T^CATGA
5267 TCATGA
SacI 2540 GAGCTC GAGCT^C
2887 GAGCTC
SapI 1547 GAAGAGC GCTCTTC(1/4)
2099 GAAGAGC
SpeI 6429 ACTAGT A^CTAGT
6441 ACTAGT
Tth111I 1228 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
2473 GACTGAGTC
Van91I 4492 CCATGGTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
6731 CCACCTCATGG
XcmI 1242 CCATAGTCAAAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1776 CCAGATTATATTTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 93 enzymes) #
#########################################
AciI AcsI AfaI AflIII AluI
Alw44I AlwI ApaLI ApoI Asp700I
AspHI AvaII BanII BbsI BbvI
BfaI BglII BmyI BpuAI BsaBI
BsaJI BseRI BsiHKAI BsiYI BslI
BsmAI BsmFI BsmI Bsp1286I Bsp1407I
BspLU11I BsrGI BsrI BssKI BstF5I
BstNI BstXI BstYI Cac8I CfoI
Cfr13I DdeI DpnII DraI EarI
EcoRII EcoT14I Fnu4HI FokI HaeIII
HhaI HinP1I HinfI HphI ItaI
Ksp632I MaeI MaeII MaeIII MamI
MboI MboII MflI MnlI MseI
MslI MvaI MwoI NdeII NlaIII
NlaIV NspI PleI RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
SspBI SspI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I XhoII XmnI