##################################
# NO CUT ENZYMES ( 48 enzymes) #
##################################
AclI AflIII AgeI AscI AseI
AsnI AspI AviII AvrII BcgI
BclI BlnI BsiWI BsmBI BsmI
BspDI BspLU11I BssHII Bst1107I BstZ17I
ClaI EcoRI EcoRV EcoT22I FbaI
FseI FspI MluI NdeI NotI
NruI NsiI PacI PflFI PinAI
PmeI PshBI Psp1406I PvuI SalI
SbfI SfiI SgrAI SnaBI SpeI
Sse8387I SwaI Tth111I
###################################
# ONE CUT ENZYMES ( 35 enzymes) #
###################################
Acc65I 2214 GGTACC G^GTACC
AccI 66 GTCTAC GT^[AC][GT]AC
AccIII 2973 TCCGGA T^CCGGA
Aor51HI 637 AGCGCT AGC^GCT
Asp718I 2214 GGTACC G^GTACC
BamHI 836 GGATCC G^GATCC
BsaBI 3091 GATGGCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 2973 TCCGGA [AT]^CCGG[AT]
BseAI 2973 TCCGGA T^CCGGA
Bsp1407I 1336 TGTACA T^GTACA
BspEI 2973 TCCGGA T^CCGGA
BspHI 2366 TCATGA T^CATGA
BsrGI 1336 TGTACA T^GTACA
BstBI 374 TTCGAA TT^CGAA
CpoI 2130 CGGACCG CG^G[AT]CCG
EagI 3180 CGGCCG C^GGCCG
EclXI 3180 CGGCCG C^GGCCG
Eco47III 637 AGCGCT AGC^GCT
Eco52I 3180 CGGCCG C^GGCCG
HindIII 266 AAGCTT A^AGCTT
KpnI 2214 GGTACC GGTAC^C
MamI 3091 GATGGCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI 2973 TCCGGA T^CCGGA
NaeI 3836 GCCGGC GCC^GGC
NgoMIV 3836 GCCGGC G^CCGGC
NheI 4764 GCTAGC G^CTAGC
NspV 374 TTCGAA TT^CGAA
PaeR7I 1193 CTCGAG C^TCGAG
RcaI 2366 TCATGA T^CATGA
RsrII 2130 CGGACCG CG^G[AT]CCG
ScaI 4382 AGTACT AGT^ACT
SexAI 3825 ACCAGGT A^CC[AT]GGT
SfuI 374 TTCGAA TT^CGAA
SspBI 1336 TGTACA T^GTACA
XhoI 1193 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 32 enzymes) #
###################################
AatII 63 GACGTC GACGT^C
2779 GACGTC
AhdI 1888 GACAGGAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
6447 GACTTGGGGTC
AspEI 1888 GACAGGAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
6447 GACTTGGGGTC
BbvCI 5254 CCTCAGC CCTCAGC(-5/-2)
5729 GCTGAGG
BciVI 1538 GTATCC GTATCC(6/5)
3507 GTATCC
BglI 2119 GCCAGGCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
2128 GCCGGACCGGC
BglII 1436 AGATCT A^GATCT
2614 AGATCT
BsiEI 1054 CGACCG CG[GA][CT]^CG
3180 CGGCCG
BsrDI 525 CATTGC GCAATG(2/0)
311 GCAATG
BsrFI 2133 ACCGGC [GA]^CCGG[CT]
3836 GCCGGC
BstEII 3159 GGTCACC G^GT[ACGT]ACC
6380 GGTGACC
BstPI 3159 GGTCACC G^GT[ACGT]ACC
6380 GGTGACC
BstXI 4573 CCAGAGGATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
6667 CCATAAACATGG
Cfr10I 2133 ACCGGC [GA]^CCGG[CT]
3836 GCCGGC
DrdI 5196 GACCCACTGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
5951 GACTGACTCGTC
Eam1105I 1888 GACAGGAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
6447 GACTTGGGGTC
EcoO65I 3159 GGTCACC G^GT[ACGT]ACC
6380 GGTGACC
HincII 5907 GTTAAC GT[CT]^[GA]AC
6581 GTTAAC
HindII 5907 GTTAAC GT[CT]^[GA]AC
6581 GTTAAC
HpaI 5907 GTTAAC GTT^AAC
6581 GTTAAC
KspI 537 CCGCGG CCGC^GG
1952 CCGCGG
MfeI 5340 CAATTG C^AATTG
5986 CAATTG
MunI 5340 CAATTG C^AATTG
5986 CAATTG
NcoI 1916 CCATGG C^CATGG
2080 CCATGG
PshAI 4806 GACTTCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
6244 GACATTTGTC
SacI 3634 GAGCTC GAGCT^C
5104 GAGCTC
SacII 537 CCGCGG CCGC^GG
1952 CCGCGG
SmaI 1486 CCCGGG CCC^GGG
5557 CCCGGG
SphI 551 GCATGC GCATG^C
5445 GCATGC
SspI 4943 AATATT AAT^ATT
6873 AATATT
XmaCI 1486 CCCGGG C^CCGGG
5557 CCCGGG
XmaI 1486 CCCGGG C^CCGGG
5557 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 143 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AflII AluI Alw44I AlwI AlwNI
AocI ApaI ApaLI ApoI Asp700I
AspHI AvaI AvaII BalI BanI
BanII BbeI BbrPI BbsI BbvI
BcnI BfaI BfrI BlpI BmyI
BpmI Bpu1102I BpuAI BsaAI BsaHI
BsaI BsaJI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BspMI BsrBI BsrI BssKI
BssSI BstAPI BstF5I BstNI BstUI
BstYI Bsu36I Cac8I CelII CfoI
Cfr13I DdeI DpnII DraI DraII
DraIII DsaI EaeI EarI Eco81I
EcoNI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI Hin1I HinP1I HinfI
HpaII HphI ItaI KasI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MluNI MnlI MscI MseI
MslI MspA1I MspI MvaI MvnI
MwoI NarI NciI NdeII NlaIII
NlaIV NspI PflMI PleI PmaCI
PmlI PpuMI PstI PvuII RsaI
SapI Sau3AI Sau96I ScrFI SfaNI
SfcI SfoI SmlI StuI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I Van91I XbaI
XcmI XhoII XmnI