##################################
# NO CUT ENZYMES ( 94 enzymes) #
##################################
AatII AccII AccIII AclI AcyI
AgeI AhdI Aor51HI ApaI AscI
AspEI AvaI AviII AvrII BalI
BbeI BbvCI BcgI BglI BglII
BlnI BsaHI BsaI BseAI BsiWI
BsoBI BspDI BspEI BspLU11I BspMI
BsrBI BssHII BssSI Bst1107I BstBI
BstEII BstPI BstUI BstZ17I CfoI
ClaI CpoI Eam1105I Eco47III EcoO65I
EcoT22I FseI FspI HaeII HgaI
HhaI Hin1I HinP1I HindIII HpaI
KasI KspI MluI MluNI MroI
MscI MvnI NarI NotI NruI
NsiI NspV PacI PaeR7I PflMI
PinAI PmeI PshAI Psp1406I PvuI
RsrII SacII SalI SbfI SexAI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SpeI SphI Sse8387I SwaI
Van91I XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 49 enzymes) #
###################################
Acc65I 2423 GGTACC G^GTACC
AflIII 2794 ACACGT A^C[GA][CT]GT
Alw44I 1049 GTGCAC G^TGCAC
AocI 2859 CCTGAGG CC^T[ACGT]AGG
ApaLI 1049 GTGCAC G^TGCAC
Asp718I 2423 GGTACC G^GTACC
AspI 2498 GACATTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BamHI 423 GGATCC G^GATCC
BbrPI 2795 CACGTG CAC^GTG
BcnI 3522 CCGGG CC^[GC]GG
BsaAI 2795 CACGTG [CT]AC^GT[GA]
BsaWI 4046 TCCGGT [AT]^CCGG[AT]
BseRI 4578 GAGGAG GAGGAG(10/8)
BsgI 514 CTGCAC GTGCAG(16/14)
BsiEI 1579 CGGCCG CG[GA][CT]^CG
BsmBI 1583 CGTCTC CGTCTC(1/5)
BsmFI 1784 GTCCC GGGAC(10/14)
BsrDI 5433 CATTGC GCAATG(2/0)
BsrFI 2740 GCCGGC [GA]^CCGG[CT]
Bsu36I 2859 CCTGAGG CC^T[ACGT]AGG
Cfr10I 2740 GCCGGC [GA]^CCGG[CT]
DraII 149 AGGACCT [GA]G^G[ACGT]CC[CT]
DraIII 3453 CACCATGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 4735 GACTTCTCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EaeI 1579 CGGCCG [CT]^GGCC[GA]
EagI 1579 CGGCCG C^GGCCG
EclXI 1579 CGGCCG C^GGCCG
Eco52I 1579 CGGCCG C^GGCCG
Eco81I 2859 CCTGAGG CC^T[ACGT]AGG
EcoNI 1274 CCTGACTAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 149 AGGACCT [GA]G^G[ACGT]CC[CT]
EcoRV 4893 GATATC GAT^ATC
FauI 5570 CCCGC CCCGC(4/6)
KpnI 2423 GGTACC GGTAC^C
MfeI 1836 CAATTG C^AATTG
MunI 1836 CAATTG C^AATTG
NaeI 2740 GCCGGC GCC^GGC
NciI 3522 CCGGG CC^[GC]GG
NdeI 5011 CATATG CA^TATG
NgoMIV 2740 GCCGGC G^CCGGC
NheI 5454 GCTAGC G^CTAGC
PflFI 2498 GACATTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 2795 CACGTG CAC^GTG
PmlI 2795 CACGTG CAC^GTG
PpuMI 149 AGGACCT [GA]G^G[AT]CC[CT]
SapI 4923 GCTCTTC GCTCTTC(1/4)
StuI 5195 AGGCCT AGG^CCT
Tth111I 2498 GACATTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XcmI 2066 CCAGATTCAAAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 19 enzymes) #
###################################
AccI 222 GTCTAC GT^[AC][GT]AC
3230 GTAGAC
AciI 2595 CCGC CCGC(-3/-1)
5571 CCGC
AflII 4730 CTTAAG C^TTAAG
4765 CTTAAG
BbsI 3218 GAAGAC GAAGAC(2/6)
4136 GAAGAC
BciVI 1622 GGATAC GTATCC(6/5)
924 GTATCC
BclI 1523 TGATCA T^GATCA
2991 TGATCA
BfrI 4730 CTTAAG C^TTAAG
4765 CTTAAG
BpuAI 3218 GAAGAC GAAGAC(2/6)
4136 GAAGAC
BsaBI 1182 GATTACTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
4893 GATATCAATC
BsmI 4714 GAATGC GAATGC(1/-1)
5601 GCATTC
BstAPI 1229 GCACTTAATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
5504 GCACCAGCTGC
BstXI 1460 CCAAGATTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1712 CCAGTCTGTTGG
DsaI 1925 CCATGG C^C[GA][CT]GG
2627 CCATGG
FbaI 1523 TGATCA T^GATCA
2991 TGATCA
MamI 1182 GATTACTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
4893 GATATCAATC
NcoI 1925 CCATGG C^CATGG
2627 CCATGG
ScaI 2430 AGTACT AGT^ACT
5239 AGTACT
TaqI 285 TCGA T^CGA
539 TCGA
TthHB8I 285 TCGA T^CGA
539 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 96 enzymes) #
#########################################
AcsI AfaI AluI AlwI AlwNI
ApoI AseI AsnI Asp700I AspHI
AvaII BanI BanII BbvI BfaI
BlpI BmyI BpmI Bpu1102I BsaJI
BsiHKAI BsiYI BslI BsmAI Bsp1286I
Bsp1407I BspHI BsrGI BsrI BssKI
BstF5I BstNI BstYI Cac8I CelII
Cfr13I DdeI DpnII DraI EarI
EcoRI EcoRII EcoT14I Fnu4HI FokI
HaeIII HapII HincII HindII HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MnlI MseI MslI MspA1I MspI
MvaI MwoI NdeII NlaIII NlaIV
NspI PleI PshBI PstI PvuII
RcaI RsaI SacI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI SspBI
SspI StyI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI XbaI XhoII
XmnI