##################################
# NO CUT ENZYMES ( 49 enzymes) #
##################################
AccIII AclI AhdI Alw44I ApaLI
AscI AspEI AvrII BcgI BglI
BlnI BsaBI BseAI BsiWI BspDI
BspEI BspHI BstBI BstEII BstPI
ClaI CpoI DrdI Eam1105I EcoO65I
EcoRI FseI HpaI MamI MfeI
MroI MunI NotI NruI NspV
PacI PmeI PpuMI PshAI Psp1406I
RcaI RsrII SbfI SfiI SfuI
SnaBI Sse8387I SwaI XcmI
###################################
# ONE CUT ENZYMES ( 44 enzymes) #
###################################
AatII 2368 GACGTC GACGT^C
AflII 4660 CTTAAG C^TTAAG
AgeI 1594 ACCGGT A^CCGGT
Aor51HI 2609 AGCGCT AGC^GCT
ApaI 93 GGGCCC GGGCC^C
AspI 6378 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 1201 TGCGCA TGC^GCA
BalI 6165 TGGCCA TGG^CCA
BamHI 1745 GGATCC G^GATCC
BbrPI 1754 CACGTG CAC^GTG
BfrI 4660 CTTAAG C^TTAAG
BsaWI 1594 ACCGGT [AT]^CCGG[AT]
BsgI 3136 GTGCAG GTGCAG(16/14)
BssSI 1233 CACGAG CACGAG(-5/-1)
Bst1107I 4768 GTATAC GTA^TAC
BstZ17I 4768 GTATAC GTA^TAC
DraI 4776 TTTAAA TTT^AAA
DraIII 4509 CACTCAGTG CAC[ACGT][ACGT][ACGT]^GTG
Eco47III 2609 AGCGCT AGC^GCT
EcoRV 5447 GATATC GAT^ATC
FspI 1201 TGCGCA TGC^GCA
MluI 950 ACGCGT A^CGCGT
MluNI 6165 TGGCCA TGG^CCA
MscI 6165 TGGCCA TGG^CCA
NcoI 5170 CCATGG C^CATGG
NdeI 4888 CATATG CA^TATG
NheI 4863 GCTAGC G^CTAGC
PaeR7I 5681 CTCGAG C^TCGAG
PflFI 6378 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 5852 CCAATAAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI 1594 ACCGGT A^CCGGT
PmaCI 1754 CACGTG CAC^GTG
PmlI 1754 CACGTG CAC^GTG
PstI 3740 CTGCAG CTGCA^G
PvuI 720 CGATCG CGAT^CG
SapI 935 GAAGAGC GCTCTTC(1/4)
ScaI 4065 AGTACT AGT^ACT
SmaI 1213 CCCGGG CCC^GGG
SphI 1204 GCATGC GCATG^C
Tth111I 6378 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 5852 CCAATAAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI 5681 CTCGAG C^TCGAG
XmaCI 1213 CCCGGG C^CCGGG
XmaI 1213 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AseI 1829 ATTAAT AT^TAAT
5040 ATTAAT
AsnI 1829 ATTAAT AT^TAAT
5040 ATTAAT
BbsI 893 GAAGAC GAAGAC(2/6)
2660 GTCTTC
BciVI 4532 GGATAC GTATCC(6/5)
5726 GTATCC
BclI 2924 TGATCA T^GATCA
3047 TGATCA
BpuAI 893 GAAGAC GAAGAC(2/6)
2660 GTCTTC
BsaAI 1754 CACGTG [CT]AC^GT[GA]
4981 TACGTG
BsmBI 3226 CGTCTC CGTCTC(1/5)
6185 CGTCTC
BsmI 3141 GAATGC GAATGC(1/-1)
3779 GAATGC
BspLU11I 2492 ACATGT A^CATGT
5754 ACATGT
BspMI 2351 ACCTGC ACCTGC(4/8)
6255 GCAGGT
BssHII 498 GCGCGC G^CGCGC
1312 GCGCGC
BstXI 3058 CCAGAGATGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
6271 CCAGCTACTTGG
EagI 85 CGGCCG C^GGCCG
1572 CGGCCG
EclXI 85 CGGCCG C^GGCCG
1572 CGGCCG
Eco52I 85 CGGCCG C^GGCCG
1572 CGGCCG
EcoNI 2808 CCTCCAGTAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
4300 CCTACTGAAGG
EcoT22I 4087 ATGCAT ATGCA^T
4284 ATGCAT
FbaI 2924 TGATCA T^GATCA
3047 TGATCA
HgaI 1661 GACGC GACGC(5/10)
1708 GCGTC
HindIII 4425 AAGCTT A^AGCTT
5186 AAGCTT
KspI 98 CCGCGG CCGC^GG
1350 CCGCGG
NsiI 4087 ATGCAT ATGCA^T
4284 ATGCAT
PshBI 1829 ATTAAT AT^TAAT
5040 ATTAAT
SacII 98 CCGCGG CCGC^GG
1350 CCGCGG
SalI 1658 GTCGAC G^TCGAC
6044 GTCGAC
SexAI 1134 ACCAGGT A^CC[AT]GGT
3156 ACCAGGT
SgrAI 1315 CGCCGGCG C[GA]^CCGG[CT]G
1423 CGCCGGCG
StuI 2115 AGGCCT AGG^CCT
2205 AGGCCT
#########################################
# 3 OR MORE CUT ENZYMES ( 136 enzymes) #
#########################################
Acc65I AccI AccII AciI AcsI
AcyI AfaI AflIII AluI AlwI
AlwNI AocI ApoI Asp700I Asp718I
AspHI AvaI AvaII BanI BanII
BbeI BbvCI BbvI BcnI BfaI
BglII BlpI BmyI BpmI Bpu1102I
BsaHI BsaI BsaJI BseRI BsiEI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I Bsp1407I BsrBI BsrDI
BsrFI BsrGI BsrI BssKI BstAPI
BstF5I BstNI BstUI BstYI Bsu36I
Cac8I CelII CfoI Cfr10I Cfr13I
DdeI DpnII DraII DsaI EaeI
EarI Eco81I EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HhaI Hin1I HinP1I HincII
HindII HinfI HpaII HphI ItaI
KasI KpnI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MnlI
MseI MslI MspA1I MspI MvaI
MvnI MwoI NaeI NarI NciI
NdeII NgoMIV NlaIII NlaIV NspI
PleI PvuII RsaI SacI Sau3AI
Sau96I ScrFI SfaNI SfcI SfoI
SmlI SpeI SspBI SspI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I XbaI XhoII
XmnI