##################################
# NO CUT ENZYMES ( 54 enzymes) #
##################################
AatII AclI AcyI AgeI AhdI
AscI AseI AsnI AspEI BbeI
BciVI BsaHI BsiEI BsmBI BspDI
BspLU11I BssHII ClaI CpoI EagI
Eam1105I EclXI Eco52I FseI Hin1I
HpaI KasI KspI MfeI MluI
MunI NarI NdeI NheI NotI
NruI PacI PaeR7I PinAI PmeI
PshBI Psp1406I PvuI RsrII SacII
SalI SbfI SfiI SfoI SgrAI
SnaBI Sse8387I SwaI XhoI
###################################
# ONE CUT ENZYMES ( 35 enzymes) #
###################################
Acc65I 628 GGTACC G^GTACC
AflII 3946 CTTAAG C^TTAAG
AocI 2196 CCTGAGG CC^T[ACGT]AGG
Aor51HI 7465 AGCGCT AGC^GCT
Asp718I 628 GGTACC G^GTACC
AviII 1975 TGCGCA TGC^GCA
AvrII 8115 CCTAGG C^CTAGG
BfrI 3946 CTTAAG C^TTAAG
BlnI 8115 CCTAGG C^CTAGG
BsaBI 276 GATGCACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsiWI 2425 CGTACG C^GTACG
BstBI 1159 TTCGAA TT^CGAA
Bsu36I 2196 CCTGAGG CC^T[ACGT]AGG
DraI 7504 TTTAAA TTT^AAA
DraIII 8088 CACCCAGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 6148 GACTATGTGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 7465 AGCGCT AGC^GCT
Eco81I 2196 CCTGAGG CC^T[ACGT]AGG
EcoRV 4546 GATATC GAT^ATC
EcoT22I 2850 ATGCAT ATGCA^T
FspI 1975 TGCGCA TGC^GCA
KpnI 628 GGTACC GGTAC^C
MamI 276 GATGCACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NaeI 461 GCCGGC GCC^GGC
NgoMIV 461 GCCGGC G^CCGGC
NsiI 2850 ATGCAT ATGCA^T
NspV 1159 TTCGAA TT^CGAA
PshAI 1047 GACCAAGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
ScaI 1529 AGTACT AGT^ACT
SexAI 3239 ACCAGGT A^CC[AT]GGT
SfuI 1159 TTCGAA TT^CGAA
SmaI 2444 CCCGGG CCC^GGG
SpeI 2149 ACTAGT A^CTAGT
XmaCI 2444 CCCGGG C^CCGGG
XmaI 2444 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 19 enzymes) #
###################################
AccIII 1310 TCCGGA T^CCGGA
2990 TCCGGA
AspI 1321 GACATCGTC GAC[ACGT]^[ACGT][ACGT]GTC
6601 GACCACGTC
BamHI 729 GGATCC G^GATCC
7085 GGATCC
BglII 2069 AGATCT A^GATCT
2663 AGATCT
BseAI 1310 TCCGGA T^CCGGA
2990 TCCGGA
BspEI 1310 TCCGGA T^CCGGA
2990 TCCGGA
BsrBI 6172 GAGCGG CCGCTC(-3/-3)
6403 GAGCGG
Bst1107I 1466 GTATAC GTA^TAC
8222 GTATAC
BstEII 636 GGTGACC G^GT[ACGT]ACC
8333 GGTGACC
BstPI 636 GGTGACC G^GT[ACGT]ACC
8333 GGTGACC
BstZ17I 1466 GTATAC GTA^TAC
8222 GTATAC
EcoO65I 636 GGTGACC G^GT[ACGT]ACC
8333 GGTGACC
HaeII 2835 GGCGCT [GA]GCGC^[CT]
7465 AGCGCT
HgaI 4057 GACGC GACGC(5/10)
7915 GACGC
HindIII 2298 AAGCTT A^AGCTT
5498 AAGCTT
MroI 1310 TCCGGA T^CCGGA
2990 TCCGGA
PflFI 1321 GACATCGTC GAC[ACGT]^[ACGT][ACGT]GTC
6601 GACCACGTC
SspI 3632 AATATT AAT^ATT
3893 AATATT
Tth111I 1321 GACATCGTC GAC[ACGT]^[ACGT][ACGT]GTC
6601 GACCACGTC
#########################################
# 3 OR MORE CUT ENZYMES ( 150 enzymes) #
#########################################
AccI AccII AciI AcsI AfaI
AflIII AluI Alw44I AlwI AlwNI
ApaI ApaLI ApoI Asp700I AspHI
AvaI AvaII BalI BanI BanII
BbrPI BbsI BbvCI BbvI BcgI
BclI BcnI BfaI BglI BlpI
BmyI BpmI Bpu1102I BpuAI BsaAI
BsaI BsaJI BsaWI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsmI BsoBI Bsp1286I Bsp1407I BspHI
BspMI BsrDI BsrFI BsrGI BsrI
BssKI BssSI BstAPI BstF5I BstNI
BstUI BstXI BstYI Cac8I CelII
CfoI Cfr10I Cfr13I DdeI DpnII
DraII DsaI EaeI EarI EcoNI
EcoO109I EcoRI EcoRII EcoT14I FauI
FbaI Fnu4HI FokI HaeIII HapII
HhaI HinP1I HincII HindII HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MluNI MnlI MscI MseI MslI
MspA1I MspI MvaI MvnI MwoI
NciI NcoI NdeII NlaIII NlaIV
NspI PflMI PleI PmaCI PmlI
PpuMI PstI PvuII RcaI RsaI
SacI SapI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI SphI SspBI
StuI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
Van91I XbaI XcmI XhoII XmnI