##################################
# NO CUT ENZYMES ( 62 enzymes) #
##################################
Acc65I AccI AccIII AgeI AhdI
AocI AscI Asp718I AspEI AviII
AvrII BglII BlnI BsaBI BsaWI
BseAI BsiEI BsiWI BspEI BspLU11I
BssHII BssSI Bst1107I BstZ17I Bsu36I
CpoI DsaI EagI Eam1105I EclXI
Eco52I Eco81I EcoRV FseI FspI
KpnI KspI MamI MluI MroI
NcoI NheI NotI NruI PacI
PaeR7I PinAI PmeI PshAI PvuI
RsrII SacI SacII SalI SbfI
SfiI SmaI SpeI Sse8387I XhoI
XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 39 enzymes) #
###################################
AatII 3044 GACGTC GACGT^C
AclI 343 AACGTT AA^CGTT
AflII 291 CTTAAG C^TTAAG
ApaI 3165 GGGCCC GGGCC^C
BamHI 3273 GGATCC G^GATCC
BbeI 2714 GGCGCC GGCGC^C
BbrPI 2199 CACGTG CAC^GTG
BbvCI 4340 CCTCAGC CCTCAGC(-5/-2)
BcgI 5706 GCAGATTTTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BcnI 3103 CCGGG CC^[GC]GG
BfrI 291 CTTAAG C^TTAAG
BglI 5737 GCCTATGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BseRI 1799 CTCCTC GAGGAG(10/8)
BsgI 1637 GTGCAG GTGCAG(16/14)
BsmBI 2229 CGTCTC CGTCTC(1/5)
BspDI 679 ATCGAT AT^CGAT
BsrBI 61 GAGCGG CCGCTC(-3/-3)
BstAPI 1381 GCAAAAAATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 2674 TTCGAA TT^CGAA
ClaI 679 ATCGAT AT^CGAT
DraIII 980 CACAAAGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 688 GACCCTCTGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
HpaI 1539 GTTAAC GTT^AAC
KasI 2714 GGCGCC G^GCGCC
NarI 2714 GGCGCC GG^CGCC
NciI 3103 CCGGG CC^[GC]GG
NdeI 4039 CATATG CA^TATG
NspV 2674 TTCGAA TT^CGAA
PmaCI 2199 CACGTG CAC^GTG
PmlI 2199 CACGTG CAC^GTG
Psp1406I 343 AACGTT AA^CGTT
ScaI 903 AGTACT AGT^ACT
SexAI 1748 ACCAGGT A^CC[AT]GGT
SfoI 2714 GGCGCC GGC^GCC
SfuI 2674 TTCGAA TT^CGAA
SgrAI 22 CACCGGCG C[GA]^CCGG[CT]G
SnaBI 5694 TACGTA TAC^GTA
SphI 5136 GCATGC GCATG^C
SwaI 3407 ATTTAAAT ATTT^AAAT
###################################
# TWO CUT ENZYMES ( 43 enzymes) #
###################################
Alw44I 497 GTGCAC G^TGCAC
2947 GTGCAC
Aor51HI 443 AGCGCT AGC^GCT
2239 AGCGCT
ApaLI 497 GTGCAC G^TGCAC
2947 GTGCAC
AseI 5091 ATTAAT AT^TAAT
5592 ATTAAT
AsnI 5091 ATTAAT AT^TAAT
5592 ATTAAT
Asp700I 2667 GAATGGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3758 GAACTCCTTC
AspI 2224 GACAGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
3181 GACAGAGTC
AvaI 593 CTCGGG C^[CT]CG[GA]G
2848 CCCGAG
BalI 2207 TGGCCA TGG^CCA
3831 TGGCCA
BanII 3112 GAGCCC G[GA]GC[CT]^C
3165 GGGCCC
BclI 2070 TGATCA T^GATCA
2810 TGATCA
BsaI 757 GAGACC GGTCTC(1/5)
1796 GGTCTC
BsoBI 593 CTCGGG C^[CT]CG[GA]G
2848 CCCGAG
Bsp1407I 781 TGTACA T^GTACA
4495 TGTACA
BspMI 1731 ACCTGC ACCTGC(4/8)
3522 GCAGGT
BsrGI 781 TGTACA T^GTACA
4495 TGTACA
BstEII 696 GGTCACC G^GT[ACGT]ACC
5354 GGTTACC
BstPI 696 GGTCACC G^GT[ACGT]ACC
5354 GGTTACC
EaeI 2207 TGGCCA [CT]^GGCC[GA]
3831 TGGCCA
Eco47III 443 AGCGCT AGC^GCT
2239 AGCGCT
EcoNI 709 CCTGGCACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1035 CCTCCCAGAGG
EcoO65I 696 GGTCACC G^GT[ACGT]ACC
5354 GGTTACC
EcoRI 3942 GAATTC G^AATTC
4362 GAATTC
EcoT22I 1388 ATGCAT ATGCA^T
4260 ATGCAT
FbaI 2070 TGATCA T^GATCA
2810 TGATCA
HgaI 2228 GCGTC GACGC(5/10)
2560 GCGTC
HindIII 642 AAGCTT A^AGCTT
5464 AAGCTT
MfeI 923 CAATTG C^AATTG
6118 CAATTG
MluNI 2207 TGGCCA TGG^CCA
3831 TGGCCA
MscI 2207 TGGCCA TGG^CCA
3831 TGGCCA
MunI 923 CAATTG C^AATTG
6118 CAATTG
NaeI 3518 GCCGGC GCC^GGC
3891 GCCGGC
NgoMIV 3518 GCCGGC G^CCGGC
3891 GCCGGC
NsiI 1388 ATGCAT ATGCA^T
4260 ATGCAT
PflFI 2224 GACAGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
3181 GACAGAGTC
PflMI 1811 CCATCGAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
6135 CCAAGTAATGG
PshBI 5091 ATTAAT AT^TAAT
5592 ATTAAT
SmlI 291 CTTAAG C^T[CT][GA]AG
998 CTCAAG
SspBI 781 TGTACA T^GTACA
4495 TGTACA
Tth111I 2224 GACAGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
3181 GACAGAGTC
Van91I 1811 CCATCGAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
6135 CCAAGTAATGG
XbaI 3988 TCTAGA T^CTAGA
4402 TCTAGA
XmnI 2667 GAATGGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3758 GAACTCCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 114 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AflIII AluI AlwI AlwNI ApoI
AspHI AvaII BanI BbsI BbvI
BciVI BfaI BlpI BmyI BpmI
Bpu1102I BpuAI BsaAI BsaHI BsaJI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsmI Bsp1286I BspHI BsrDI BsrFI
BsrI BssKI BstF5I BstNI BstUI
BstXI BstYI Cac8I CelII CfoI
Cfr10I Cfr13I DdeI DpnII DraI
DraII EarI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HhaI Hin1I HinP1I HincII
HindII HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MnlI MseI MslI
MspA1I MspI MvaI MvnI MwoI
NdeII NlaIII NlaIV NspI PleI
PpuMI PstI PvuII RcaI RsaI
SapI Sau3AI Sau96I ScrFI SfaNI
SfcI SspI StuI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I XcmI XhoII