##################################
# NO CUT ENZYMES ( 53 enzymes) #
##################################
AatII AccI AccIII AflII AgeI
AhdI AscI AseI AsnI AspEI
AviII AvrII BfrI BlnI BseAI
BsiWI BspDI BspEI BssHII Bst1107I
BstBI BstEII BstPI BstZ17I ClaI
CpoI DraIII Eam1105I EcoO65I EcoRV
FseI FspI HpaI MluI MroI
NotI NruI NspV PacI PinAI
PmeI PshBI PvuI RsrII SalI
SbfI ScaI SfiI SfuI SgrAI
SpeI Sse8387I SwaI
###################################
# ONE CUT ENZYMES ( 33 enzymes) #
###################################
Acc65I 6189 GGTACC G^GTACC
AclI 1316 AACGTT AA^CGTT
AocI 6383 CCTAAGG CC^T[ACGT]AGG
Aor51HI 2739 AGCGCT AGC^GCT
Asp718I 6189 GGTACC G^GTACC
BamHI 1675 GGATCC G^GATCC
BbrPI 3493 CACGTG CAC^GTG
BciVI 4703 GTATCC GTATCC(6/5)
BglII 4749 AGATCT A^GATCT
BsaWI 261 ACCGGA [AT]^CCGG[AT]
BsrDI 1558 GCAATG GCAATG(2/0)
Bsu36I 6383 CCTAAGG CC^T[ACGT]AGG
Eco47III 2739 AGCGCT AGC^GCT
Eco81I 6383 CCTAAGG CC^T[ACGT]AGG
EcoRI 3466 GAATTC G^AATTC
EcoT22I 4504 ATGCAT ATGCA^T
HindIII 5704 AAGCTT A^AGCTT
KpnI 6189 GGTACC GGTAC^C
MfeI 2168 CAATTG C^AATTG
MunI 2168 CAATTG C^AATTG
NaeI 3220 GCCGGC GCC^GGC
NdeI 3627 CATATG CA^TATG
NgoMIV 3220 GCCGGC G^CCGGC
NsiI 4504 ATGCAT ATGCA^T
PaeR7I 1247 CTCGAG C^TCGAG
PmaCI 3493 CACGTG CAC^GTG
PmlI 3493 CACGTG CAC^GTG
PshAI 3154 GACCCTTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Psp1406I 1316 AACGTT AA^CGTT
SexAI 3673 ACCAGGT A^CC[AT]GGT
SspI 6146 AATATT AAT^ATT
XbaI 6497 TCTAGA T^CTAGA
XhoI 1247 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
ApaI 1346 GGGCCC GGGCC^C
5887 GGGCCC
BbeI 2709 GGCGCC GGCGC^C
4882 GGCGCC
BbvCI 3284 GCTGAGG CCTCAGC(-5/-2)
6220 GCTGAGG
BsiEI 1046 CGGCCG CG[GA][CT]^CG
1719 CGGCCG
BsmI 3528 GAATGC GAATGC(1/-1)
1215 GCATTC
BssSI 461 CACGAG CACGAG(-5/-1)
6717 CACGAG
DrdI 5860 GACAGGCCAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
5953 GACACTCAGGTC
EagI 1046 CGGCCG C^GGCCG
1719 CGGCCG
EclXI 1046 CGGCCG C^GGCCG
1719 CGGCCG
Eco52I 1046 CGGCCG C^GGCCG
1719 CGGCCG
HincII 2996 GTCAAC GT[CT]^[GA]AC
3170 GTTGAC
HindII 2996 GTCAAC GT[CT]^[GA]AC
3170 GTTGAC
KasI 2709 GGCGCC G^GCGCC
4882 GGCGCC
KspI 63 CCGCGG CCGC^GG
3521 CCGCGG
NarI 2709 GGCGCC GG^CGCC
4882 GGCGCC
NheI 1747 GCTAGC G^CTAGC
5236 GCTAGC
SacI 4397 GAGCTC GAGCT^C
5824 GAGCTC
SacII 63 CCGCGG CCGC^GG
3521 CCGCGG
SapI 5906 GAAGAGC GCTCTTC(1/4)
991 GCTCTTC
SfoI 2709 GGCGCC GGC^GCC
4882 GGCGCC
SmaI 3586 CCCGGG CCC^GGG
5474 CCCGGG
SnaBI 497 TACGTA TAC^GTA
3685 TACGTA
SphI 3201 GCATGC GCATG^C
5469 GCATGC
XmaCI 3586 CCCGGG C^CCGGG
5474 CCCGGG
XmaI 3586 CCCGGG C^CCGGG
5474 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 147 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AflIII AluI Alw44I AlwI AlwNI
ApaLI ApoI Asp700I AspHI AspI
AvaI AvaII BalI BanI BanII
BbsI BbvI BcgI BclI BcnI
BfaI BglI BlpI BmyI BpmI
Bpu1102I BpuAI BsaAI BsaBI BsaHI
BsaI BsaJI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmBI BsmFI
BsoBI Bsp1286I Bsp1407I BspHI BspLU11I
BspMI BsrBI BsrFI BsrGI BsrI
BssKI BstAPI BstF5I BstNI BstUI
BstXI BstYI Cac8I CelII CfoI
Cfr10I Cfr13I DdeI DpnII DraI
DraII DsaI EaeI EarI EcoNI
EcoO109I EcoRII EcoT14I FauI FbaI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI Hin1I HinP1I HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MamI MboI MboII
MflI MluNI MnlI MscI MseI
MslI MspA1I MspI MvaI MvnI
MwoI NciI NcoI NdeII NlaIII
NlaIV NspI PflFI PflMI PleI
PpuMI PstI PvuII RcaI RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI SspBI StuI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI Tth111I TthHB8I Van91I XcmI
XhoII XmnI