##################################
# NO CUT ENZYMES ( 56 enzymes) #
##################################
AccIII AclI AgeI Aor51HI AscI
AviII BbrPI BglII BsaAI BsaWI
BseAI BsiEI BsiWI BsmBI BsmI
BspDI BspEI BssHII BstBI ClaI
CpoI DrdI EagI EclXI Eco47III
Eco52I FseI FspI HpaI KspI
MfeI MluI MroI MunI NdeI
NheI NotI NruI NspV PacI
PinAI PmaCI PmeI PmlI Psp1406I
PvuI RsrII SacII SalI SbfI
SfiI SfuI SnaBI SpeI Sse8387I
SwaI
###################################
# ONE CUT ENZYMES ( 45 enzymes) #
###################################
Acc65I 513 GGTACC G^GTACC
AccII 3216 CGCG CG^CG
AflII 3884 CTTAAG C^TTAAG
AhdI 3279 GACTGTCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 2721 CCTCAGG CC^T[ACGT]AGG
Asp718I 513 GGTACC G^GTACC
AspEI 3279 GACTGTCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 2224 GACTGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 773 CCTAGG C^CTAGG
BamHI 1960 GGATCC G^GATCC
BbeI 27 GGCGCC GGCGC^C
BcgI 1166 GCAGCATGCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfrI 3884 CTTAAG C^TTAAG
BglI 213 GCCCACAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlnI 773 CCTAGG C^CTAGG
BlpI 402 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 402 GCTGAGC GC^T[ACGT]AGC
BsaBI 5307 GATTAGTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BssSI 806 CACGAG CACGAG(-5/-1)
Bst1107I 3740 GTATAC GTA^TAC
BstUI 3216 CGCG CG^CG
BstZ17I 3740 GTATAC GTA^TAC
Bsu36I 2721 CCTCAGG CC^T[ACGT]AGG
CelII 402 GCTGAGC GC^T[ACGT]AGC
Eam1105I 3279 GACTGTCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 2721 CCTCAGG CC^T[ACGT]AGG
EcoRV 2276 GATATC GAT^ATC
EcoT22I 5826 ATGCAT ATGCA^T
FauI 5362 CCCGC CCCGC(4/6)
KasI 27 GGCGCC G^GCGCC
KpnI 513 GGTACC GGTAC^C
MamI 5307 GATTAGTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MvnI 3216 CGCG CG^CG
NarI 27 GGCGCC GG^CGCC
NsiI 5826 ATGCAT ATGCA^T
PaeR7I 972 CTCGAG C^TCGAG
PflFI 2224 GACTGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
SapI 1860 GAAGAGC GCTCTTC(1/4)
ScaI 5991 AGTACT AGT^ACT
SexAI 4950 ACCTGGT A^CC[AT]GGT
SfoI 27 GGCGCC GGC^GCC
SgrAI 1953 CACCGGCG C[GA]^CCGG[CT]G
SphI 1169 GCATGC GCATG^C
Tth111I 2224 GACTGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoI 972 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 17 enzymes) #
###################################
ApaI 2056 GGGCCC GGGCC^C
3532 GGGCCC
AseI 3714 ATTAAT AT^TAAT
5984 ATTAAT
AsnI 3714 ATTAAT AT^TAAT
5984 ATTAAT
Asp700I 254 GAACCTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
4958 GAAGGTATTC
BciVI 4946 GGATAC GTATCC(6/5)
5312 GTATCC
BspHI 2110 TCATGA T^CATGA
4776 TCATGA
BsrBI 200 CCGCTC CCGCTC(-3/-3)
2018 CCGCTC
DraIII 5334 CACTGGGTG CAC[ACGT][ACGT][ACGT]^GTG
6125 CACAAAGTG
EcoNI 131 CCTGCTGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
425 CCTCCTCCAGG
EcoRI 1941 GAATTC G^AATTC
2332 GAATTC
HindIII 2864 AAGCTT A^AGCTT
5835 AAGCTT
NaeI 154 GCCGGC GCC^GGC
3450 GCCGGC
NgoMIV 154 GCCGGC G^CCGGC
3450 GCCGGC
PshAI 1542 GACCAGCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
4403 GACTACAGTC
PshBI 3714 ATTAAT AT^TAAT
5984 ATTAAT
RcaI 2110 TCATGA T^CATGA
4776 TCATGA
XmnI 254 GAACCTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
4958 GAAGGTATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 140 enzymes) #
#########################################
AatII AccI AciI AcsI AcyI
AfaI AflIII AluI Alw44I AlwI
AlwNI ApaLI ApoI AspHI AvaI
AvaII BalI BanI BanII BbsI
BbvCI BbvI BclI BcnI BfaI
BmyI BpmI BpuAI BsaHI BsaI
BsaJI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
Bsp1407I BspLU11I BspMI BsrDI BsrFI
BsrGI BsrI BssKI BstAPI BstEII
BstF5I BstNI BstPI BstXI BstYI
Cac8I CfoI Cfr10I Cfr13I DdeI
DpnII DraI DraII DsaI EaeI
EarI EcoO109I EcoO65I EcoRII EcoT14I
FbaI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI Hin1I HinP1I
HincII HindII HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MluNI MnlI
MscI MseI MslI MspA1I MspI
MvaI MwoI NciI NcoI NdeII
NlaIII NlaIV NspI PflMI PleI
PpuMI PstI PvuII RsaI SacI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmaI SmlI SspBI SspI StuI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I Van91I
XbaI XcmI XhoII XmaCI XmaI