##################################
# NO CUT ENZYMES ( 54 enzymes) #
##################################
AatII Acc65I AccIII AclI AflII
Aor51HI AscI AseI AsnI Asp718I
AvrII BclI BfrI BlnI BseAI
BsiWI BsmI BspDI BspEI BspLU11I
BssSI Bst1107I BstZ17I ClaI CpoI
Eco47III EcoRV FbaI FseI HindIII
HpaI KpnI MfeI MroI MunI
NdeI NheI NotI NruI PacI
PaeR7I PmeI PshBI Psp1406I PvuI
RsrII SalI SfiI SnaBI SphI
SspI SwaI XbaI XhoI
###################################
# ONE CUT ENZYMES ( 45 enzymes) #
###################################
AccI 3085 GTCTAC GT^[AC][GT]AC
AgeI 1031 ACCGGT A^CCGGT
AhdI 2140 GACTGGTCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 3102 GTGCAC G^TGCAC
ApaLI 3102 GTGCAC G^TGCAC
AspEI 2140 GACTGGTCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BamHI 193 GGATCC G^GATCC
BbrPI 1237 CACGTG CAC^GTG
BcgI 4140 CGATCCCCGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 3373 GGATAC GTATCC(6/5)
BglII 3128 AGATCT A^GATCT
BsaBI 3293 GATGCCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsiEI 43 CGGCCG CG[GA][CT]^CG
Bsp1407I 2198 TGTACA T^GTACA
BsrGI 2198 TGTACA T^GTACA
BssHII 1938 GCGCGC G^CGCGC
BstAPI 4331 GCAGGGCGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 649 TTCGAA TT^CGAA
BstEII 2481 GGTGACC G^GT[ACGT]ACC
BstPI 2481 GGTGACC G^GT[ACGT]ACC
DraI 5114 TTTAAA TTT^AAA
EagI 43 CGGCCG C^GGCCG
Eam1105I 2140 GACTGGTCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 43 CGGCCG C^GGCCG
Eco52I 43 CGGCCG C^GGCCG
EcoO65I 2481 GGTGACC G^GT[ACGT]ACC
EcoT22I 5060 ATGCAT ATGCA^T
HincII 454 GTCAAC GT[CT]^[GA]AC
HindII 454 GTCAAC GT[CT]^[GA]AC
MamI 3293 GATGCCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluI 2837 ACGCGT A^CGCGT
NaeI 3257 GCCGGC GCC^GGC
NgoMIV 3257 GCCGGC G^CCGGC
NsiI 5060 ATGCAT ATGCA^T
NspV 649 TTCGAA TT^CGAA
PinAI 1031 ACCGGT A^CCGGT
PmaCI 1237 CACGTG CAC^GTG
PmlI 1237 CACGTG CAC^GTG
PshAI 447 GACGTTTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SbfI 624 CCTGCAGG CCTGCA^GG
SfuI 649 TTCGAA TT^CGAA
SgrAI 3256 CGCCGGCG C[GA]^CCGG[CT]G
SpeI 244 ACTAGT A^CTAGT
Sse8387I 624 CCTGCAGG CCTGCA^GG
SspBI 2198 TGTACA T^GTACA
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AflIII 221 ACGTGT A^C[GA][CT]GT
2837 ACGCGT
AspI 3845 GACAGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
4313 GACCTGGTC
AviII 2614 TGCGCA TGC^GCA
4788 TGCGCA
BalI 3425 TGGCCA TGG^CCA
3513 TGGCCA
BsaWI 1031 ACCGGT [AT]^CCGG[AT]
1688 ACCGGA
BspHI 1383 TCATGA T^CATGA
1727 TCATGA
EcoRI 1057 GAATTC G^AATTC
4386 GAATTC
FspI 2614 TGCGCA TGC^GCA
4788 TGCGCA
KspI 40 CCGCGG CCGC^GG
2114 CCGCGG
MluNI 3425 TGGCCA TGG^CCA
3513 TGGCCA
MscI 3425 TGGCCA TGG^CCA
3513 TGGCCA
PflFI 3845 GACAGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
4313 GACCTGGTC
RcaI 1383 TCATGA T^CATGA
1727 TCATGA
SacII 40 CCGCGG CCGC^GG
2114 CCGCGG
SapI 1779 GCTCTTC GCTCTTC(1/4)
4022 GCTCTTC
ScaI 563 AGTACT AGT^ACT
1321 AGTACT
SexAI 2564 ACCTGGT A^CC[AT]GGT
4314 ACCTGGT
TaqI 650 TCGA T^CGA
818 TCGA
Tth111I 3845 GACAGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
4313 GACCTGGTC
TthHB8I 650 TCGA T^CGA
818 TCGA
XcmI 1206 CCAGGCAGCCTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
4194 CCAGGCCCACCTTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 138 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AluI AlwI AlwNI AocI ApaI
ApoI Asp700I AspHI AvaI AvaII
BanI BanII BbeI BbsI BbvCI
BbvI BcnI BfaI BglI BlpI
BmyI BpmI Bpu1102I BpuAI BsaAI
BsaHI BsaI BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmBI
BsmFI BsoBI Bsp1286I BspMI BsrBI
BsrDI BsrFI BsrI BssKI BstF5I
BstNI BstUI BstXI BstYI Bsu36I
Cac8I CelII CfoI Cfr10I Cfr13I
DdeI DpnII DraII DraIII DrdI
DsaI EaeI EarI Eco81I EcoNI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI Hin1I HinP1I HinfI HpaII
HphI ItaI KasI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MnlI MseI MslI MspA1I MspI
MvaI MvnI MwoI NarI NciI
NcoI NdeII NlaIII NlaIV NspI
PflMI PleI PpuMI PstI PvuII
RsaI SacI Sau3AI Sau96I ScrFI
SfaNI SfcI SfoI SmaI SmlI
StuI StyI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI Van91I XhoII
XmaCI XmaI XmnI