##################################
# NO CUT ENZYMES ( 86 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AflII AgeI AlwNI Aor51HI AscI
AseI AsnI Asp700I Asp718I AviII
AvrII BciVI BclI BfrI BlnI
BsaBI BsaWI BseAI BsiWI Bsp1407I
BspDI BspEI BspHI BspLU11I BsrBI
BsrGI BssHII Bst1107I BstBI BstZ17I
ClaI CpoI DraIII Eco47III EcoRV
FbaI FseI FspI HgaI HpaI
KpnI KspI MamI MfeI MluI
MroI MunI NaeI NgoMIV NheI
NotI NruI NspV PacI PaeR7I
PflMI PinAI PmeI PshBI Psp1406I
PvuI RcaI RsrII SacII SalI
SbfI ScaI SfiI SfuI SgrAI
SmaI SnaBI Sse8387I SspBI SwaI
Van91I XbaI XhoI XmaCI XmaI
XmnI
###################################
# ONE CUT ENZYMES ( 45 enzymes) #
###################################
AccII 805 CGCG CG^CG
AcyI 1557 GGCGCC G[GA]^CG[CT]C
AhdI 3969 GACAGTGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 3998 GTGCAC G^TGCAC
ApaLI 3998 GTGCAC G^TGCAC
AspEI 3969 GACAGTGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 411 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BbeI 1557 GGCGCC GGCGC^C
BbrPI 4344 CACGTG CAC^GTG
BbvCI 4623 GCTGAGG CCTCAGC(-5/-2)
BglI 4646 GCCTGGGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 21 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 21 GCTGAGC GC^T[ACGT]AGC
BsaAI 4344 CACGTG [CT]AC^GT[GA]
BsaHI 1557 GGCGCC G[GA]^CG[CT]C
BsiEI 4442 CGGCCG CG[GA][CT]^CG
BsmBI 390 GAGACG CGTCTC(1/5)
BssSI 4630 CACGAG CACGAG(-5/-1)
BstEII 498 GGTGACC G^GT[ACGT]ACC
BstPI 498 GGTGACC G^GT[ACGT]ACC
BstUI 805 CGCG CG^CG
CelII 21 GCTGAGC GC^T[ACGT]AGC
DraI 3125 TTTAAA TTT^AAA
DrdI 3144 GACCATCCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 4442 CGGCCG C^GGCCG
Eam1105I 3969 GACAGTGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 4442 CGGCCG C^GGCCG
Eco52I 4442 CGGCCG C^GGCCG
EcoO65I 498 GGTGACC G^GT[ACGT]ACC
Hin1I 1557 GGCGCC G[GA]^CG[CT]C
HincII 408 GTTGAC GT[CT]^[GA]AC
HindII 408 GTTGAC GT[CT]^[GA]AC
KasI 1557 GGCGCC G^GCGCC
MvnI 805 CGCG CG^CG
NarI 1557 GGCGCC GG^CGCC
NdeI 3804 CATATG CA^TATG
PflFI 411 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 4344 CACGTG CAC^GTG
PmlI 4344 CACGTG CAC^GTG
PshAI 3159 GACCTAAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SapI 1796 GCTCTTC GCTCTTC(1/4)
SexAI 580 ACCTGGT A^CC[AT]GGT
SfoI 1557 GGCGCC GGC^GCC
SpeI 3644 ACTAGT A^CTAGT
Tth111I 411 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AflIII 4343 ACACGT A^C[GA][CT]GT
4345 ACGTGT
ApaI 3262 GGGCCC GGGCC^C
4212 GGGCCC
BalI 1108 TGGCCA TGG^CCA
4533 TGGCCA
BamHI 2337 GGATCC G^GATCC
2937 GGATCC
BbsI 3479 GAAGAC GAAGAC(2/6)
704 GTCTTC
BcgI 1670 GCAGAAACGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
4629 GCACGAGAATCG
BcnI 3 CCGGG CC^[GC]GG
4445 CCGGG
BpuAI 3479 GAAGAC GAAGAC(2/6)
704 GTCTTC
BsrFI 930 ACCGGC [GA]^CCGG[CT]
4440 ACCGGC
BstXI 2830 CCACATCTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4536 CCAACATGGTGG
Cfr10I 930 ACCGGC [GA]^CCGG[CT]
4440 ACCGGC
EcoT22I 2269 ATGCAT ATGCA^T
2631 ATGCAT
HaeII 1557 GGCGCC [GA]GCGC^[CT]
1861 AGCGCC
HindIII 1053 AAGCTT A^AGCTT
1806 AAGCTT
MluNI 1108 TGGCCA TGG^CCA
4533 TGGCCA
MscI 1108 TGGCCA TGG^CCA
4533 TGGCCA
NciI 3 CCGGG CC^[GC]GG
4445 CCGGG
NsiI 2269 ATGCAT ATGCA^T
2631 ATGCAT
PvuII 1269 CAGCTG CAG^CTG
4066 CAGCTG
SacI 660 GAGCTC GAGCT^C
1890 GAGCTC
SphI 3284 GCATGC GCATG^C
4594 GCATGC
SspI 3719 AATATT AAT^ATT
4399 AATATT
StuI 672 AGGCCT AGG^CCT
2313 AGGCCT
#########################################
# 3 OR MORE CUT ENZYMES ( 104 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AocI ApoI AspHI AvaI AvaII
BanI BanII BbvI BfaI BglII
BmyI BpmI BsaI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI BsmI BsoBI Bsp1286I BspMI
BsrDI BsrI BssKI BstAPI BstF5I
BstNI BstYI Bsu36I Cac8I CfoI
Cfr13I DdeI DpnII DraII DsaI
EaeI EarI Eco81I EcoNI EcoO109I
EcoRI EcoRII EcoT14I FauI Fnu4HI
FokI HaeIII HapII HhaI HinP1I
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MnlI MseI MslI MspA1I
MspI MvaI MwoI NcoI NdeII
NlaIII NlaIV NspI PleI PpuMI
PstI RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I XcmI XhoII