##################################
# NO CUT ENZYMES ( 78 enzymes) #
##################################
AatII AccI AclI AgeI AhdI
Aor51HI AscI AseI AsnI AspEI
BbeI BbrPI BcgI BciVI BclI
BglI BglII BsaAI BsaBI BsiEI
BsiWI BspDI BssHII BssSI Bst1107I
BstBI BstZ17I ClaI CpoI DrdI
EagI Eam1105I EclXI Eco47III Eco52I
EcoRV EcoT22I FbaI FseI HaeII
HincII HindII HindIII HpaI KasI
KspI MaeII MamI MluI NaeI
NarI NgoMIV NotI NruI NsiI
NspV PacI PaeR7I PinAI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PvuI RsrII SacI SacII SalI
ScaI SfiI SfoI SfuI SgrAI
SnaBI SwaI XhoI
###################################
# ONE CUT ENZYMES ( 33 enzymes) #
###################################
Acc65I 4374 GGTACC G^GTACC
AccII 342 CGCG CG^CG
AccIII 2411 TCCGGA T^CCGGA
AflII 4658 CTTAAG C^TTAAG
Asp718I 4374 GGTACC G^GTACC
AviII 400 TGCGCA TGC^GCA
BfrI 4658 CTTAAG C^TTAAG
BseAI 2411 TCCGGA T^CCGGA
Bsp1407I 4008 TGTACA T^GTACA
BspEI 2411 TCCGGA T^CCGGA
BsrGI 4008 TGTACA T^GTACA
BstAPI 1729 GCACTCAGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI 342 CGCG CG^CG
BstXI 3706 CCACTCCTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DraI 53 TTTAAA TTT^AAA
DraIII 1730 CACTCAGTG CAC[ACGT][ACGT][ACGT]^GTG
FspI 400 TGCGCA TGC^GCA
KpnI 4374 GGTACC GGTAC^C
MfeI 187 CAATTG C^AATTG
MroI 2411 TCCGGA T^CCGGA
MunI 187 CAATTG C^AATTG
MvnI 342 CGCG CG^CG
NcoI 3195 CCATGG C^CATGG
NdeI 3154 CATATG CA^TATG
NheI 361 GCTAGC G^CTAGC
SbfI 2967 CCTGCAGG CCTGCA^GG
SpeI 4520 ACTAGT A^CTAGT
SphI 1922 GCATGC GCATG^C
Sse8387I 2967 CCTGCAGG CCTGCA^GG
SspBI 4008 TGTACA T^GTACA
TaqI 748 TCGA T^CGA
TthHB8I 748 TCGA T^CGA
XbaI 3761 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AcyI 42 GGCGTC G[GA]^CG[CT]C
4680 GGCGTC
AflIII 1566 ACATGT A^C[GA][CT]GT
3416 ACATGT
Alw44I 2306 GTGCAC G^TGCAC
3631 GTGCAC
ApaLI 2306 GTGCAC G^TGCAC
3631 GTGCAC
Asp700I 2373 GAAGAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
4117 GAAAATTTTC
AspI 217 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
1606 GACTATGTC
AvrII 3870 CCTAGG C^CTAGG
4082 CCTAGG
BamHI 606 GGATCC G^GATCC
3907 GGATCC
BlnI 3870 CCTAGG C^CTAGG
4082 CCTAGG
BsaHI 42 GGCGTC G[GA]^CG[CT]C
4680 GGCGTC
BsaI 215 GAGACC GGTCTC(1/5)
4252 GGTCTC
BsmBI 44 CGTCTC CGTCTC(1/5)
210 GAGACG
BsmI 1240 GCATTC GAATGC(1/-1)
3778 GCATTC
BspHI 1820 TCATGA T^CATGA
4204 TCATGA
BspLU11I 1566 ACATGT A^CATGT
3416 ACATGT
BspMI 3321 ACCTGC ACCTGC(4/8)
897 GCAGGT
BsrBI 3094 CCGCTC CCGCTC(-3/-3)
3133 GAGCGG
FauI 692 GCGGG CCCGC(4/6)
3135 GCGGG
Hin1I 42 GGCGTC G[GA]^CG[CT]C
4680 GGCGTC
PflFI 217 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
1606 GACTATGTC
RcaI 1820 TCATGA T^CATGA
4204 TCATGA
SapI 832 GAAGAGC GCTCTTC(1/4)
3096 GCTCTTC
SexAI 218 ACCAGGT A^CC[AT]GGT
3173 ACCAGGT
SmaI 276 CCCGGG CCC^GGG
2342 CCCGGG
SspI 3208 AATATT AAT^ATT
4451 AATATT
Tth111I 217 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
1606 GACTATGTC
XmaCI 276 CCCGGG C^CCGGG
2342 CCCGGG
XmaI 276 CCCGGG C^CCGGG
2342 CCCGGG
XmnI 2373 GAAGAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
4117 GAAAATTTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 118 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AlwNI AocI ApaI ApoI AspHI
AvaI AvaII BalI BanI BanII
BbsI BbvCI BbvI BcnI BfaI
BlpI BmyI BpmI Bpu1102I BpuAI
BsaJI BsaWI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BsrDI BsrFI BsrI BssKI
BstEII BstF5I BstNI BstPI BstYI
Bsu36I Cac8I CelII CfoI Cfr10I
Cfr13I DdeI DpnII DraII DsaI
EaeI EarI Eco81I EcoNI EcoO109I
EcoO65I EcoRI EcoRII EcoT14I Fnu4HI
FokI HaeIII HapII HgaI HhaI
HinP1I HinfI HpaII HphI ItaI
Ksp632I MaeI MaeIII MboI MboII
MflI MluNI MnlI MscI MseI
MslI MspA1I MspI MvaI MwoI
NciI NdeII NlaIII NlaIV NspI
PflMI PleI PpuMI PstI PvuII
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI StuI StyI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
Van91I XcmI XhoII