##################################
# NO CUT ENZYMES ( 49 enzymes) #
##################################
Acc65I AflII AscI AseI AsnI
Asp700I Asp718I BclI BfrI BsaBI
BsiWI BspLU11I BsrDI Bst1107I BstBI
BstZ17I DrdI EcoRI EcoRV EcoT22I
FbaI FseI HindIII HpaI KpnI
MamI MfeI MunI NheI NotI
NruI NsiI NspV PacI PaeR7I
PmeI PshAI PshBI PvuI SfiI
SfuI SgrAI SnaBI SpeI SspI
SwaI XbaI XhoI XmnI
###################################
# ONE CUT ENZYMES ( 36 enzymes) #
###################################
AclI 4488 AACGTT AA^CGTT
AgeI 1559 ACCGGT A^CCGGT
Alw44I 4911 GTGCAC G^TGCAC
Aor51HI 279 AGCGCT AGC^GCT
ApaLI 4911 GTGCAC G^TGCAC
AspI 233 GACATCGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 4440 CCTAGG C^CTAGG
BamHI 4055 GGATCC G^GATCC
BciVI 3041 GGATAC GTATCC(6/5)
BlnI 4440 CCTAGG C^CTAGG
Bsp1407I 4930 TGTACA T^GTACA
BspDI 665 ATCGAT AT^CGAT
BspHI 387 TCATGA T^CATGA
BsrGI 4930 TGTACA T^GTACA
BstEII 4741 GGTGACC G^GT[ACGT]ACC
BstPI 4741 GGTGACC G^GT[ACGT]ACC
ClaI 665 ATCGAT AT^CGAT
CpoI 4021 CGGACCG CG^G[AT]CCG
Eco47III 279 AGCGCT AGC^GCT
EcoO65I 4741 GGTGACC G^GT[ACGT]ACC
MluI 5168 ACGCGT A^CGCGT
NcoI 4313 CCATGG C^CATGG
NdeI 4934 CATATG CA^TATG
PflFI 233 GACATCGTC GAC[ACGT]^[ACGT][ACGT]GTC
PinAI 1559 ACCGGT A^CCGGT
Psp1406I 4488 AACGTT AA^CGTT
RcaI 387 TCATGA T^CATGA
RsrII 4021 CGGACCG CG^G[AT]CCG
SalI 841 GTCGAC G^TCGAC
SapI 2653 GCTCTTC GCTCTTC(1/4)
ScaI 2223 AGTACT AGT^ACT
SexAI 4737 ACCTGGT A^CC[AT]GGT
SspBI 4930 TGTACA T^GTACA
StuI 2062 AGGCCT AGG^CCT
Tth111I 233 GACATCGTC GAC[ACGT]^[ACGT][ACGT]GTC
XcmI 4728 CCACCTGCCACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AatII 833 GACGTC GACGT^C
1441 GACGTC
AflIII 4365 ACGTGT A^C[GA][CT]GT
5168 ACGCGT
AhdI 833 GACGTCCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1148 GACGCAGCGTC
AspEI 833 GACGTCCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1148 GACGCAGCGTC
BbsI 3865 GAAGAC GAAGAC(2/6)
1652 GTCTTC
BfaI 4441 CTAG C^TAG
4886 CTAG
BpuAI 3865 GAAGAC GAAGAC(2/6)
1652 GTCTTC
BsmBI 5221 CGTCTC CGTCTC(1/5)
1439 GAGACG
BstXI 3201 CCAAGAAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
5016 CCAGCACAGTGG
DraIII 4287 CACTAAGTG CAC[ACGT][ACGT][ACGT]^GTG
4736 CACCTGGTG
EagI 207 CGGCCG C^GGCCG
3630 CGGCCG
Eam1105I 833 GACGTCCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1148 GACGCAGCGTC
EclXI 207 CGGCCG C^GGCCG
3630 CGGCCG
Eco52I 207 CGGCCG C^GGCCG
3630 CGGCCG
HincII 841 GTCGAC GT[CT]^[GA]AC
2056 GTCAAC
HindII 841 GTCGAC GT[CT]^[GA]AC
2056 GTCAAC
KspI 1897 CCGCGG CCGC^GG
2019 CCGCGG
MaeI 4441 CTAG C^TAG
4886 CTAG
PflMI 1446 CCATGAAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3106 CCACTCACTGG
SacII 1897 CCGCGG CCGC^GG
2019 CCGCGG
SphI 138 GCATGC GCATG^C
5120 GCATGC
Van91I 1446 CCATGAAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3106 CCACTCACTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 151 enzymes) #
#########################################
AccI AccII AccIII AciI AcsI
AcyI AfaI AluI AlwI AlwNI
AocI ApaI ApoI AspHI AvaI
AvaII AviII BalI BanI BanII
BbeI BbrPI BbvCI BbvI BcgI
BcnI BglI BglII BlpI BmyI
BpmI Bpu1102I BsaAI BsaHI BsaI
BsaJI BsaWI BseAI BseRI BsgI
BsiEI BsiHKAI BsiYI BslI BsmAI
BsmFI BsmI BsoBI Bsp1286I BspEI
BspMI BsrBI BsrFI BsrI BssHII
BssKI BssSI BstAPI BstF5I BstNI
BstUI BstYI Bsu36I Cac8I CelII
CfoI Cfr10I Cfr13I DdeI DpnII
DraI DraII DsaI EaeI EarI
Eco81I EcoNI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI FspI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HinfI HpaII HphI ItaI
KasI Ksp632I MaeII MaeIII MboI
MboII MflI MluNI MnlI MroI
MscI MseI MslI MspA1I MspI
MvaI MvnI MwoI NaeI NarI
NciI NdeII NgoMIV NlaIII NlaIV
NspI PleI PmaCI PmlI PpuMI
PstI PvuII RsaI SacI Sau3AI
Sau96I SbfI ScrFI SfaNI SfcI
SfoI SmaI SmlI Sse8387I StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I XhoII XmaCI
XmaI