##################################
# NO CUT ENZYMES ( 69 enzymes) #
##################################
Acc65I AclI AflII AgeI AhdI
Aor51HI AscI AseI AsnI Asp718I
AspEI AspI AviII AvrII BamHI
BbeI BfrI BlnI BsiWI BsmBI
BsmI BspDI BssHII Bst1107I BstBI
BstEII BstPI BstZ17I ClaI CpoI
DrdI Eam1105I Eco47III EcoO65I EcoRI
EcoT22I FseI FspI HaeII KasI
KpnI MluI NarI NdeI NheI
NotI NruI NsiI NspV PacI
PaeR7I PflFI PinAI PmeI PshAI
PshBI Psp1406I PvuI RsrII SfoI
SfuI SgrAI SnaBI SpeI SspI
SwaI Tth111I XbaI XhoI
###################################
# ONE CUT ENZYMES ( 40 enzymes) #
###################################
AatII 854 GACGTC GACGT^C
AccI 3667 GTCGAC GT^[AC][GT]AC
Alw44I 3802 GTGCAC G^TGCAC
AocI 1130 CCTCAGG CC^T[ACGT]AGG
ApaLI 3802 GTGCAC G^TGCAC
BanI 4134 GGCACC G^G[CT][GA]CC
BbrPI 4176 CACGTG CAC^GTG
BbsI 951 GAAGAC GAAGAC(2/6)
BbvCI 3400 GCTGAGG CCTCAGC(-5/-2)
BcgI 2920 CGACAGATTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 3267 GTATCC GTATCC(6/5)
BlpI 3015 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 3015 GCTGAGC GC^T[ACGT]AGC
BpuAI 951 GAAGAC GAAGAC(2/6)
BsaAI 4176 CACGTG [CT]AC^GT[GA]
BspHI 1731 TCATGA T^CATGA
BspLU11I 3101 ACATGT A^CATGT
BsrDI 3276 CATTGC GCAATG(2/0)
BssSI 3139 CACGAG CACGAG(-5/-1)
Bsu36I 1130 CCTCAGG CC^T[ACGT]AGG
CelII 3015 GCTGAGC GC^T[ACGT]AGC
DraI 3487 TTTAAA TTT^AAA
DraIII 1561 CACACAGTG CAC[ACGT][ACGT][ACGT]^GTG
Eco81I 1130 CCTCAGG CC^T[ACGT]AGG
EcoRV 1756 GATATC GAT^ATC
HindIII 4396 AAGCTT A^AGCTT
HpaI 1375 GTTAAC GTT^AAC
MfeI 4300 CAATTG C^AATTG
MunI 4300 CAATTG C^AATTG
NcoI 2471 CCATGG C^CATGG
PmaCI 4176 CACGTG CAC^GTG
PmlI 4176 CACGTG CAC^GTG
RcaI 1731 TCATGA T^CATGA
SalI 3667 GTCGAC G^TCGAC
SbfI 3186 CCTGCAGG CCTGCA^GG
ScaI 2207 AGTACT AGT^ACT
SexAI 1034 ACCAGGT A^CC[AT]GGT
SfiI 65 GGCCGCCTGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SphI 2361 GCATGC GCATG^C
Sse8387I 3186 CCTGCAGG CCTGCA^GG
###################################
# TWO CUT ENZYMES ( 14 enzymes) #
###################################
AcyI 854 GACGTC G[GA]^CG[CT]C
1368 GGCGTC
AflIII 448 ACGTGT A^C[GA][CT]GT
3101 ACATGT
BglI 66 GCCGCCTGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
496 GCCAGCATGGC
BglII 3548 AGATCT A^GATCT
4311 AGATCT
BsaHI 854 GACGTC G[GA]^CG[CT]C
1368 GGCGTC
BsaI 2544 GGTCTC GGTCTC(1/5)
2958 GGTCTC
BsrBI 4017 CCGCTC CCGCTC(-3/-3)
441 GAGCGG
BstAPI 1041 GCAGACAGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
3149 GCACAGGCTGC
EagI 64 CGGCCG C^GGCCG
1416 CGGCCG
EclXI 64 CGGCCG C^GGCCG
1416 CGGCCG
Eco52I 64 CGGCCG C^GGCCG
1416 CGGCCG
Hin1I 854 GACGTC G[GA]^CG[CT]C
1368 GGCGTC
NspI 2361 GCATGC [GA]CATG^[CT]
3101 ACATGT
SmlI 2169 CTTGAG C^T[CT][GA]AG
4307 CTTGAG
#########################################
# 3 OR MORE CUT ENZYMES ( 135 enzymes) #
#########################################
AccII AccIII AciI AcsI AfaI
AluI AlwI AlwNI ApaI ApoI
Asp700I AspHI AvaI AvaII BalI
BanII BbvI BclI BcnI BfaI
BmyI BpmI BsaBI BsaJI BsaWI
BseAI BseRI BsgI BsiEI BsiHKAI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I Bsp1407I BspEI BspMI BsrFI
BsrGI BsrI BssKI BstF5I BstNI
BstUI BstXI BstYI Cac8I CfoI
Cfr10I Cfr13I DdeI DpnII DraII
DsaI EaeI EarI EcoNI EcoO109I
EcoRII EcoT14I FauI FbaI Fnu4HI
FokI HaeIII HapII HgaI HhaI
HinP1I HincII HindII HinfI HpaII
HphI ItaI Ksp632I KspI MaeI
MaeII MaeIII MamI MboI MboII
MflI MluNI MnlI MroI MscI
MseI MslI MspA1I MspI MvaI
MvnI MwoI NaeI NciI NdeII
NgoMIV NlaIII NlaIV PflMI PleI
PpuMI PstI PvuII RsaI SacI
SacII SapI Sau3AI Sau96I ScrFI
SfaNI SfcI SmaI SspBI StuI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I Van91I
XcmI XhoII XmaCI XmaI XmnI