##################################
# NO CUT ENZYMES ( 65 enzymes) #
##################################
AccI AccII AccIII AhdI AscI
AspEI AspI AviII BciVI BclI
BseAI BsiWI BspEI BspHI BsrBI
BssHII Bst1107I BstEII BstPI BstUI
BstZ17I CpoI DrdI Eam1105I EcoO65I
EcoRI EcoRV EcoT22I FbaI FseI
FspI HpaI KspI MfeI MluI
MroI MunI MvnI NdeI NheI
NotI NruI NsiI PacI PflFI
PmeI PshAI RcaI RsrII SacII
SalI SapI SbfI ScaI SexAI
SfiI SmaI SnaBI SpeI Sse8387I
SwaI Tth111I XbaI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 55 enzymes) #
###################################
AatII 1 GACGTC GACGT^C
Acc65I 2708 GGTACC G^GTACC
AclI 1659 AACGTT AA^CGTT
AcsI 1610 AAATTT [GA]^AATT[CT]
AflII 5309 CTTAAG C^TTAAG
AgeI 1620 ACCGGT A^CCGGT
Aor51HI 1429 AGCGCT AGC^GCT
ApaI 2822 GGGCCC GGGCC^C
ApoI 1610 AAATTT [GA]^AATT[CT]
AseI 5717 ATTAAT AT^TAAT
AsnI 5717 ATTAAT AT^TAAT
Asp718I 2708 GGTACC G^GTACC
AvaI 1080 CTCGAG C^[CT]CG[GA]G
AvrII 2242 CCTAGG C^CTAGG
BbeI 4466 GGCGCC GGCGC^C
BbrPI 4159 CACGTG CAC^GTG
BfrI 5309 CTTAAG C^TTAAG
BlnI 2242 CCTAGG C^CTAGG
BsaAI 4159 CACGTG [CT]AC^GT[GA]
BsaWI 1620 ACCGGT [AT]^CCGG[AT]
BsmBI 5481 CGTCTC CGTCTC(1/5)
BsoBI 1080 CTCGAG C^[CT]CG[GA]G
BspDI 5698 ATCGAT AT^CGAT
BssSI 1577 CTCGTG CACGAG(-5/-1)
BstBI 2634 TTCGAA TT^CGAA
ClaI 5698 ATCGAT AT^CGAT
DraI 728 TTTAAA TTT^AAA
DraIII 5018 CACTTGGTG CAC[ACGT][ACGT][ACGT]^GTG
EagI 1466 CGGCCG C^GGCCG
EclXI 1466 CGGCCG C^GGCCG
Eco47III 1429 AGCGCT AGC^GCT
Eco52I 1466 CGGCCG C^GGCCG
HgaI 3340 GCGTC GACGC(5/10)
HincII 1063 GTCAAC GT[CT]^[GA]AC
HindII 1063 GTCAAC GT[CT]^[GA]AC
KasI 4466 GGCGCC G^GCGCC
KpnI 2708 GGTACC GGTAC^C
NarI 4466 GGCGCC GG^CGCC
NcoI 2883 CCATGG C^CATGG
NspV 2634 TTCGAA TT^CGAA
PaeR7I 1080 CTCGAG C^TCGAG
PflMI 1510 CCAGATTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI 1620 ACCGGT A^CCGGT
PmaCI 4159 CACGTG CAC^GTG
PmlI 4159 CACGTG CAC^GTG
PshBI 5717 ATTAAT AT^TAAT
Psp1406I 1659 AACGTT AA^CGTT
PvuI 1043 CGATCG CGAT^CG
SacI 827 GAGCTC GAGCT^C
SfoI 4466 GGCGCC GGC^GCC
SfuI 2634 TTCGAA TT^CGAA
SgrAI 2177 CACCGGCG C[GA]^CCGG[CT]G
StuI 2445 AGGCCT AGG^CCT
Van91I 1510 CCAGATTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI 1080 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 13 enzymes) #
###################################
AcyI 1 GACGTC G[GA]^CG[CT]C
4466 GGCGCC
BamHI 2221 GGATCC G^GATCC
4551 GGATCC
BbsI 2434 GAAGAC GAAGAC(2/6)
4110 GAAGAC
BcgI 2008 CGACAGCTGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
4144 CGAGCTGCCTGC
BpuAI 2434 GAAGAC GAAGAC(2/6)
4110 GAAGAC
BsaHI 1 GACGTC G[GA]^CG[CT]C
4466 GGCGCC
BsiEI 1043 CGATCG CG[GA][CT]^CG
1466 CGGCCG
BspLU11I 1844 ACATGT A^CATGT
2787 ACATGT
FauI 945 CCCGC CCCGC(4/6)
3279 CCCGC
Hin1I 1 GACGTC G[GA]^CG[CT]C
4466 GGCGCC
HindIII 1023 AAGCTT A^AGCTT
1178 AAGCTT
SphI 1444 GCATGC GCATG^C
5399 GCATGC
SspI 5128 AATATT AAT^ATT
5720 AATATT
#########################################
# 3 OR MORE CUT ENZYMES ( 125 enzymes) #
#########################################
AciI AfaI AflIII AluI Alw44I
AlwI AlwNI AocI ApaLI Asp700I
AspHI AvaII BalI BanI BanII
BbvCI BbvI BcnI BfaI BglI
BglII BlpI BmyI BpmI Bpu1102I
BsaBI BsaI BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsmI Bsp1286I Bsp1407I BspMI BsrDI
BsrFI BsrGI BsrI BssKI BstAPI
BstF5I BstNI BstXI BstYI Bsu36I
Cac8I CelII CfoI Cfr10I Cfr13I
DdeI DpnII DraII DsaI EaeI
EarI Eco81I EcoNI EcoO109I EcoRII
EcoT14I Fnu4HI FokI HaeII HaeIII
HapII HhaI HinP1I HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MamI MboI MboII MflI
MluNI MnlI MscI MseI MslI
MspA1I MspI MvaI MwoI NaeI
NciI NdeII NgoMIV NlaIII NlaIV
NspI PleI PpuMI PstI PvuII
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI SspBI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I XcmI XhoII XmnI