##################################
# NO CUT ENZYMES ( 48 enzymes) #
##################################
AatII AgeI AhdI Alw44I ApaLI
AscI AspEI AspI AviII BbeI
BcgI BclI BsiWI BspLU11I BssHII
BssSI BstBI Eam1105I EcoRI EcoRV
FbaI FseI FspI KasI MfeI
MluI MunI NarI NotI NruI
NspV PacI PaeR7I PflFI PinAI
PmeI PvuI SapI SfiI SfoI
SfuI SgrAI SmaI SwaI Tth111I
XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 64 enzymes) #
###################################
Acc65I 2919 GGTACC G^GTACC
AccIII 4841 TCCGGA T^CCGGA
AclI 1010 AACGTT AA^CGTT
AcyI 2949 GACGCC G[GA]^CG[CT]C
AflII 1992 CTTAAG C^TTAAG
Aor51HI 665 AGCGCT AGC^GCT
Asp700I 2288 GAACATCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 2919 GGTACC G^GTACC
AvrII 3903 CCTAGG C^CTAGG
BbrPI 5294 CACGTG CAC^GTG
BbvCI 4361 GCTGAGG CCTCAGC(-5/-2)
BciVI 728 GGATAC GTATCC(6/5)
BfrI 1992 CTTAAG C^TTAAG
BglI 1231 GCCACAGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 1416 AGATCT A^GATCT
BlnI 3903 CCTAGG C^CTAGG
BsaHI 2949 GACGCC G[GA]^CG[CT]C
BseAI 4841 TCCGGA T^CCGGA
BsmBI 2447 CGTCTC CGTCTC(1/5)
Bsp1407I 3788 TGTACA T^GTACA
BspDI 1445 ATCGAT AT^CGAT
BspEI 4841 TCCGGA T^CCGGA
BspHI 1647 TCATGA T^CATGA
BspMI 4252 ACCTGC ACCTGC(4/8)
BsrBI 930 CCGCTC CCGCTC(-3/-3)
BsrGI 3788 TGTACA T^GTACA
Bst1107I 1951 GTATAC GTA^TAC
BstAPI 3947 GCAGCCACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstZ17I 1951 GTATAC GTA^TAC
ClaI 1445 ATCGAT AT^CGAT
CpoI 5447 CGGACCG CG^G[AT]CCG
DraIII 3001 CACAGCGTG CAC[ACGT][ACGT][ACGT]^GTG
Eco47III 665 AGCGCT AGC^GCT
EcoNI 3480 CCTGAGCTAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT22I 347 ATGCAT ATGCA^T
Hin1I 2949 GACGCC G[GA]^CG[CT]C
HpaI 3683 GTTAAC GTT^AAC
KpnI 2919 GGTACC GGTAC^C
KspI 3282 CCGCGG CCGC^GG
MroI 4841 TCCGGA T^CCGGA
NaeI 2303 GCCGGC GCC^GGC
NdeI 1159 CATATG CA^TATG
NgoMIV 2303 GCCGGC G^CCGGC
NheI 792 GCTAGC G^CTAGC
NsiI 347 ATGCAT ATGCA^T
PmaCI 5294 CACGTG CAC^GTG
PmlI 5294 CACGTG CAC^GTG
PshAI 3415 GACGTGGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Psp1406I 1010 AACGTT AA^CGTT
RcaI 1647 TCATGA T^CATGA
RsrII 5447 CGGACCG CG^G[AT]CCG
SacI 4533 GAGCTC GAGCT^C
SacII 3282 CCGCGG CCGC^GG
SalI 911 GTCGAC G^TCGAC
SbfI 3126 CCTGCAGG CCTGCA^GG
ScaI 2871 AGTACT AGT^ACT
SexAI 4476 ACCTGGT A^CC[AT]GGT
SnaBI 4414 TACGTA TAC^GTA
SpeI 3179 ACTAGT A^CTAGT
SphI 2888 GCATGC GCATG^C
Sse8387I 3126 CCTGCAGG CCTGCA^GG
SspBI 3788 TGTACA T^GTACA
StuI 5092 AGGCCT AGG^CCT
XmnI 2288 GAACATCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 33 enzymes) #
###################################
AcsI 125 AAATTT [GA]^AATT[CT]
3223 GAATTT
AflIII 5293 ACACGT A^C[GA][CT]GT
5295 ACGTGT
AocI 5095 CCTGAGG CC^T[ACGT]AGG
5252 CCTTAGG
ApoI 125 AAATTT [GA]^AATT[CT]
3223 GAATTT
AseI 175 ATTAAT AT^TAAT
2801 ATTAAT
AsnI 175 ATTAAT AT^TAAT
2801 ATTAAT
AspHI 3183 GTGCTC G[AT]GC[AT]^C
4533 GAGCTC
AvaI 3021 CTCGGG C^[CT]CG[GA]G
5164 CTCGGG
BamHI 4844 GGATCC G^GATCC
5248 GGATCC
BlpI 1273 GCTCAGC GC^T[ACGT]AGC
3113 GCTCAGC
Bpu1102I 1273 GCTCAGC GC^T[ACGT]AGC
3113 GCTCAGC
BsaAI 4414 TACGTA [CT]AC^GT[GA]
5294 CACGTG
BsaBI 943 GATGAGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1438 GATGCCAATC
BsaWI 3316 TCCGGT [AT]^CCGG[AT]
4841 TCCGGA
BsiHKAI 3183 GTGCTC G[AT]GC[AT]^C
4533 GAGCTC
BsoBI 3021 CTCGGG C^[CT]CG[GA]G
5164 CTCGGG
BstEII 433 GGTAACC G^GT[ACGT]ACC
4480 GGTCACC
BstPI 433 GGTAACC G^GT[ACGT]ACC
4480 GGTCACC
Bsu36I 5095 CCTGAGG CC^T[ACGT]AGG
5252 CCTTAGG
CelII 1273 GCTCAGC GC^T[ACGT]AGC
3113 GCTCAGC
DrdI 2226 GACAGCCCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3440 GACGGGGCTGTC
EagI 3279 CGGCCG C^GGCCG
3453 CGGCCG
EclXI 3279 CGGCCG C^GGCCG
3453 CGGCCG
Eco52I 3279 CGGCCG C^GGCCG
3453 CGGCCG
Eco81I 5095 CCTGAGG CC^T[ACGT]AGG
5252 CCTTAGG
EcoO65I 433 GGTAACC G^GT[ACGT]ACC
4480 GGTCACC
HgaI 2949 GACGC GACGC(5/10)
2413 GCGTC
HincII 911 GTCGAC GT[CT]^[GA]AC
3683 GTTAAC
HindII 911 GTCGAC GT[CT]^[GA]AC
3683 GTTAAC
HindIII 2975 AAGCTT A^AGCTT
5390 AAGCTT
MamI 943 GATGAGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1438 GATGCCAATC
PshBI 175 ATTAAT AT^TAAT
2801 ATTAAT
XcmI 1833 CCAAGAGGAGATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
4346 CCAGCCCGATTGTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 113 enzymes) #
#########################################
AccI AccII AciI AfaI AluI
AlwI AlwNI ApaI AvaII BalI
BanI BanII BbsI BbvI BcnI
BfaI BmyI BpmI BpuAI BsaI
BsaJI BseRI BsgI BsiEI BsiYI
BslI BsmAI BsmFI BsmI Bsp1286I
BsrDI BsrFI BsrI BssKI BstF5I
BstNI BstUI BstXI BstYI Cac8I
CfoI Cfr10I Cfr13I DdeI DpnII
DraI DraII DsaI EaeI EarI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HhaI
HinP1I HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MluNI MnlI MscI
MseI MslI MspA1I MspI MvaI
MvnI MwoI NciI NcoI NdeII
NlaIII NlaIV NspI PflMI PleI
PpuMI PstI PvuII RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
SspI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
Van91I XbaI XhoII