##################################
# NO CUT ENZYMES ( 64 enzymes) #
##################################
AatII Acc65I AccIII AclI AcyI
AflII AgeI Alw44I AlwNI ApaLI
AscI Asp718I AviII BbeI BciVI
BfrI BsaHI BseAI BsiEI BsiWI
BspDI BspEI BspHI BsrBI BssHII
BstAPI ClaI CpoI DraI EagI
EclXI Eco52I EcoNI EcoT22I FseI
FspI Hin1I HpaI KasI KpnI
KspI MroI NarI NheI NotI
NruI NsiI PacI PinAI PmeI
Psp1406I PvuI RcaI RsrII SacII
SalI SapI SfiI SfoI SgrAI
SnaBI SpeI SphI SwaI
###################################
# ONE CUT ENZYMES ( 49 enzymes) #
###################################
AccII 2618 CGCG CG^CG
AhdI 2209 GACCCCAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Aor51HI 3401 AGCGCT AGC^GCT
ApaI 3021 GGGCCC GGGCC^C
AseI 4490 ATTAAT AT^TAAT
AsnI 4490 ATTAAT AT^TAAT
AspEI 2209 GACCCCAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BbrPI 3652 CACGTG CAC^GTG
BcgI 1164 CGATGACTGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglI 3440 GCCATGTTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 1844 AGATCT A^GATCT
Bsp1407I 5280 TGTACA T^GTACA
BspLU11I 4483 ACATGT A^CATGT
BsrGI 5280 TGTACA T^GTACA
BssSI 2542 CACGAG CACGAG(-5/-1)
Bst1107I 906 GTATAC GTA^TAC
BstEII 3931 GGTAACC G^GT[ACGT]ACC
BstPI 3931 GGTAACC G^GT[ACGT]ACC
BstUI 2618 CGCG CG^CG
BstZ17I 906 GTATAC GTA^TAC
DraIII 2011 CACCGGGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 4836 GACTCTAGGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 2209 GACCCCAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 3401 AGCGCT AGC^GCT
EcoO65I 3931 GGTAACC G^GT[ACGT]ACC
EcoRI 3253 GAATTC G^AATTC
HincII 2557 GTCAAC GT[CT]^[GA]AC
HindII 2557 GTCAAC GT[CT]^[GA]AC
MfeI 1597 CAATTG C^AATTG
MluI 2617 ACGCGT A^CGCGT
MunI 1597 CAATTG C^AATTG
MvnI 2618 CGCG CG^CG
NaeI 90 GCCGGC GCC^GGC
NdeI 4896 CATATG CA^TATG
NgoMIV 90 GCCGGC G^CCGGC
PflMI 880 CCAACAGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 3652 CACGTG CAC^GTG
PmlI 3652 CACGTG CAC^GTG
PshBI 4490 ATTAAT AT^TAAT
SbfI 579 CCTGCAGG CCTGCA^GG
ScaI 2098 AGTACT AGT^ACT
SexAI 1500 ACCAGGT A^CC[AT]GGT
SmaI 4946 CCCGGG CCC^GGG
Sse8387I 579 CCTGCAGG CCTGCA^GG
SspBI 5280 TGTACA T^GTACA
SspI 5275 AATATT AAT^ATT
Van91I 880 CCAACAGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmaCI 4946 CCCGGG C^CCGGG
XmaI 4946 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AccI 906 GTATAC GT^[AC][GT]AC
2221 GTAGAC
Asp700I 2096 GAAGTACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
4592 GAAGTAGTTC
AspI 849 GACGGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
2344 GACTGTGTC
AvrII 3168 CCTAGG C^CTAGG
4755 CCTAGG
BamHI 575 GGATCC G^GATCC
2799 GGATCC
BbvCI 1249 GCTGAGG CCTCAGC(-5/-2)
1729 GCTGAGG
BlnI 3168 CCTAGG C^CTAGG
4755 CCTAGG
BlpI 764 GCTCAGC GC^T[ACGT]AGC
3867 GCTCAGC
Bpu1102I 764 GCTCAGC GC^T[ACGT]AGC
3867 GCTCAGC
BsaAI 3365 CACGTA [CT]AC^GT[GA]
3652 CACGTG
BsaBI 2766 GATTGTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
4951 GATGGCTATC
BsaWI 572 ACCGGA [AT]^CCGG[AT]
1586 ACCGGA
BsrFI 90 GCCGGC [GA]^CCGG[CT]
3119 ACCGGC
CelII 764 GCTCAGC GC^T[ACGT]AGC
3867 GCTCAGC
Cfr10I 90 GCCGGC [GA]^CCGG[CT]
3119 ACCGGC
MamI 2766 GATTGTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
4951 GATGGCTATC
PaeR7I 23 CTCGAG C^TCGAG
3102 CTCGAG
PflFI 849 GACGGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
2344 GACTGTGTC
PshAI 378 GACTGTTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
4913 GACTCTTGTC
Tth111I 849 GACGGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
2344 GACTGTGTC
XbaI 1911 TCTAGA T^CTAGA
5155 TCTAGA
XhoI 23 CTCGAG C^TCGAG
3102 CTCGAG
XmnI 2096 GAAGTACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
4592 GAAGTAGTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 122 enzymes) #
#########################################
AciI AcsI AfaI AflIII AluI
AlwI AocI ApoI AspHI AvaI
AvaII BalI BanI BanII BbsI
BbvI BclI BcnI BfaI BmyI
BpmI BpuAI BsaI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmBI BsmFI BsmI BsoBI Bsp1286I
BspMI BsrDI BsrI BssKI BstBI
BstF5I BstNI BstXI BstYI Bsu36I
Cac8I CfoI Cfr13I DdeI DpnII
DraII DsaI EaeI EarI Eco81I
EcoO109I EcoRII EcoRV EcoT14I FauI
FbaI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI HinP1I HindIII
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MluNI MnlI MscI MseI
MslI MspA1I MspI MvaI MwoI
NciI NcoI NdeII NlaIII NlaIV
NspI NspV PleI PpuMI PstI
PvuII RsaI SacI Sau3AI Sau96I
ScrFI SfaNI SfcI SfuI SmlI
StuI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
XcmI XhoII