##################################
# NO CUT ENZYMES ( 75 enzymes) #
##################################
Acc65I AccI AccIII AclI AflII
Alw44I Aor51HI ApaLI AscI Asp718I
AspI AvrII BcgI BciVI BfrI
BlnI BlpI Bpu1102I BsaBI BseAI
BsiWI BsmBI BspDI BspEI BspHI
BspLU11I BssHII BssSI Bst1107I BstBI
BstZ17I CelII ClaI CpoI DraIII
DrdI EagI EclXI Eco47III Eco52I
EcoRI EcoRV EcoT22I FseI KpnI
KspI MamI MfeI MluI MroI
MunI NheI NotI NruI NsiI
NspV PacI PaeR7I PflFI PmeI
Psp1406I PvuI RcaI RsrII SacII
SalI ScaI SfuI SgrAI SnaBI
SpeI SwaI Tth111I XbaI XhoI
###################################
# ONE CUT ENZYMES ( 33 enzymes) #
###################################
AatII 3931 GACGTC GACGT^C
AflIII 4098 ACGTGT A^C[GA][CT]GT
AgeI 1577 ACCGGT A^CCGGT
AhdI 862 GACAACAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AseI 197 ATTAAT AT^TAAT
AsnI 197 ATTAAT AT^TAAT
AspEI 862 GACAACAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII 2994 TGCGCA TGC^GCA
BbeI 1079 GGCGCC GGCGC^C
BbrPI 3698 CACGTG CAC^GTG
BglII 3909 AGATCT A^GATCT
BsaAI 3698 CACGTG [CT]AC^GT[GA]
BsmI 3982 GAATGC GAATGC(1/-1)
BsrDI 2433 GCAATG GCAATG(2/0)
BstAPI 952 GCAGTCTGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Eam1105I 862 GACAACAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
FspI 2994 TGCGCA TGC^GCA
HpaI 360 GTTAAC GTT^AAC
KasI 1079 GGCGCC G^GCGCC
NaeI 1374 GCCGGC GCC^GGC
NarI 1079 GGCGCC GG^CGCC
NdeI 5609 CATATG CA^TATG
NgoMIV 1374 GCCGGC G^CCGGC
PinAI 1577 ACCGGT A^CCGGT
PmaCI 3698 CACGTG CAC^GTG
PmlI 3698 CACGTG CAC^GTG
PshBI 197 ATTAAT AT^TAAT
SbfI 4913 CCTGCAGG CCTGCA^GG
SfiI 1522 GGCCAGGGTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 1079 GGCGCC GGC^GCC
Sse8387I 4913 CCTGCAGG CCTGCA^GG
SspI 1841 AATATT AAT^ATT
StuI 5537 AGGCCT AGG^CCT
###################################
# TWO CUT ENZYMES ( 6 enzymes) #
###################################
BsiEI 1003 CGACCG CG[GA][CT]^CG
2511 CGACCG
DraI 330 TTTAAA TTT^AAA
522 TTTAAA
PshAI 1751 GACCCCAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
4580 GACATGGGTC
SacI 1164 GAGCTC GAGCT^C
3959 GAGCTC
SexAI 2726 ACCAGGT A^CC[AT]GGT
4173 ACCAGGT
SphI 4836 GCATGC GCATG^C
5555 GCATGC
#########################################
# 3 OR MORE CUT ENZYMES ( 144 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AluI AlwI AlwNI AocI ApaI
ApoI Asp700I AspHI AvaI AvaII
BalI BamHI BanI BanII BbsI
BbvCI BbvI BclI BcnI BfaI
BglI BmyI BpmI BpuAI BsaHI
BsaI BsaJI BsaWI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I Bsp1407I BspMI BsrBI
BsrFI BsrGI BsrI BssKI BstEII
BstF5I BstNI BstPI BstUI BstXI
BstYI Bsu36I Cac8I CfoI Cfr10I
Cfr13I DdeI DpnII DraII DsaI
EaeI EarI Eco81I EcoNI EcoO109I
EcoO65I EcoRII EcoT14I FauI FbaI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI Hin1I HinP1I HincII
HindII HindIII HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MluNI MnlI
MscI MseI MslI MspA1I MspI
MvaI MvnI MwoI NciI NcoI
NdeII NlaIII NlaIV NspI PflMI
PleI PpuMI PstI PvuII RsaI
SapI Sau3AI Sau96I ScrFI SfaNI
SfcI SmaI SmlI SspBI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I Van91I XcmI
XhoII XmaCI XmaI XmnI