##################################
# NO CUT ENZYMES ( 73 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AflII AgeI AhdI Asp718I AspEI
AvaI AviII AvrII BamHI BbeI
BbrPI BcgI BfrI BlnI BsaWI
BseAI BsiWI BsmBI BsmI BsoBI
BspEI BsrFI BssSI Bst1107I BstZ17I
Cfr10I CpoI Eam1105I EcoRV EcoT22I
FseI FspI KasI KpnI KspI
MluI MroI NaeI NarI NgoMIV
NheI NotI NruI NsiI PacI
PaeR7I PinAI PmaCI PmeI PmlI
Psp1406I PvuI RsrII SacII SalI
SbfI SexAI SfiI SfoI SgrAI
SmaI SnaBI SphI Sse8387I SwaI
XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 43 enzymes) #
###################################
AccII 1568 CGCG CG^CG
AcyI 134 GACGCC G[GA]^CG[CT]C
Alw44I 7931 GTGCAC G^TGCAC
Aor51HI 4130 AGCGCT AGC^GCT
ApaI 5503 GGGCCC GGGCC^C
ApaLI 7931 GTGCAC G^TGCAC
AscI 1566 GGCGCGCC GG^CGCGCC
AspI 3577 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BbvCI 436 CCTCAGC CCTCAGC(-5/-2)
BcnI 840 CCCGG CC^[GC]GG
BglI 4 GCCATGCGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 7895 GCTAAGC GC^T[ACGT]AGC
Bpu1102I 7895 GCTAAGC GC^T[ACGT]AGC
BsaHI 134 GACGCC G[GA]^CG[CT]C
BsiEI 4379 CGGCCG CG[GA][CT]^CG
Bsp1407I 6918 TGTACA T^GTACA
BsrBI 1240 GAGCGG CCGCTC(-3/-3)
BsrDI 3660 CATTGC GCAATG(2/0)
BsrGI 6918 TGTACA T^GTACA
BssHII 1567 GCGCGC G^CGCGC
BstAPI 3442 GCATTGGATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI 1568 CGCG CG^CG
CelII 7895 GCTAAGC GC^T[ACGT]AGC
DrdI 1514 GACCAAGCCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 4379 CGGCCG C^GGCCG
EclXI 4379 CGGCCG C^GGCCG
Eco47III 4130 AGCGCT AGC^GCT
Eco52I 4379 CGGCCG C^GGCCG
EcoRI 2159 GAATTC G^AATTC
HapII 841 CCGG C^CGG
Hin1I 134 GACGCC G[GA]^CG[CT]C
HpaI 3895 GTTAAC GTT^AAC
HpaII 841 CCGG C^CGG
MfeI 1538 CAATTG C^AATTG
MspI 841 CCGG C^CGG
MunI 1538 CAATTG C^AATTG
MvnI 1568 CGCG CG^CG
NciI 840 CCCGG CC^[GC]GG
PflFI 3577 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
ScaI 6588 AGTACT AGT^ACT
SspBI 6918 TGTACA T^GTACA
StuI 3608 AGGCCT AGG^CCT
Tth111I 3577 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
BalI 2554 TGGCCA TGG^CCA
6747 TGGCCA
BciVI 1672 GGATAC GTATCC(6/5)
2146 GTATCC
BglII 1035 AGATCT A^GATCT
2603 AGATCT
BsaAI 3311 TACGTG [CT]AC^GT[GA]
7480 TACGTG
BsaI 1512 GAGACC GGTCTC(1/5)
2266 GAGACC
BspDI 989 ATCGAT AT^CGAT
2278 ATCGAT
BspLU11I 1246 ACATGT A^CATGT
7040 ACATGT
BstEII 4939 GGTCACC G^GT[ACGT]ACC
6838 GGTCACC
BstPI 4939 GGTCACC G^GT[ACGT]ACC
6838 GGTCACC
ClaI 989 ATCGAT AT^CGAT
2278 ATCGAT
DraIII 3857 CACATAGTG CAC[ACGT][ACGT][ACGT]^GTG
4500 CACAGTGTG
EcoO65I 4939 GGTCACC G^GT[ACGT]ACC
6838 GGTCACC
FauI 5016 CCCGC CCCGC(4/6)
9 GCGGG
HincII 3135 GTCAAC GT[CT]^[GA]AC
3895 GTTAAC
HindII 3135 GTCAAC GT[CT]^[GA]AC
3895 GTTAAC
MluNI 2554 TGGCCA TGG^CCA
6747 TGGCCA
MscI 2554 TGGCCA TGG^CCA
6747 TGGCCA
NcoI 1550 CCATGG C^CATGG
3797 CCATGG
NdeI 199 CATATG CA^TATG
3631 CATATG
PshAI 197 GACATATGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
4756 GACCTTTGTC
SapI 2719 GAAGAGC GCTCTTC(1/4)
3510 GAAGAGC
SmlI 964 CTTGAG C^T[CT][GA]AG
7762 CTCAAG
SpeI 262 ACTAGT A^CTAGT
4490 ACTAGT
XcmI 4281 CCAAAGGCTGGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
5525 CCAAGGGTGGAGTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 118 enzymes) #
#########################################
AciI AcsI AfaI AflIII AluI
AlwI AlwNI AocI ApoI AseI
AsnI Asp700I AspHI AvaII BanI
BanII BbsI BbvI BclI BfaI
BmyI BpmI BpuAI BsaBI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI Bsp1286I BspHI BspMI
BsrI BssKI BstBI BstF5I BstNI
BstXI BstYI Bsu36I Cac8I CfoI
Cfr13I DdeI DpnII DraI DraII
DsaI EaeI EarI Eco81I EcoNI
EcoO109I EcoRII EcoT14I FbaI Fnu4HI
FokI HaeII HaeIII HgaI HhaI
HinP1I HindIII HinfI HphI ItaI
Ksp632I MaeI MaeII MaeIII MamI
MboI MboII MflI MnlI MseI
MslI MspA1I MvaI MwoI NdeII
NlaIII NlaIV NspI NspV PflMI
PleI PpuMI PshBI PstI PvuII
RcaI RsaI SacI Sau3AI Sau96I
ScrFI SfaNI SfcI SfuI SspI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I Van91I
XbaI XhoII XmnI