##################################
# NO CUT ENZYMES ( 73 enzymes) #
##################################
AatII AccIII AgeI AhdI Alw44I
Aor51HI ApaI ApaLI AscI AseI
AsnI AspEI AspI AviII AvrII
BbeI BbrPI BbvCI BglI BglII
BlnI BsaAI BsaBI BseAI BspDI
BspEI BssHII BssSI Bst1107I BstBI
BstZ17I ClaI EagI Eam1105I EclXI
Eco47III Eco52I FseI FspI KasI
KspI MamI MluI MroI NarI
NotI NruI NspV PacI PaeR7I
PflFI PinAI PmaCI PmeI PmlI
PshBI PvuI SacI SacII SbfI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SpeI Sse8387I SwaI Tth111I
XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 41 enzymes) #
###################################
AclI 1569 AACGTT AA^CGTT
AcyI 3556 GGCGTC G[GA]^CG[CT]C
AflIII 3109 ACATGT A^C[GA][CT]GT
AocI 3172 CCTGAGG CC^T[ACGT]AGG
AspHI 4146 GAGCAC G[AT]GC[AT]^C
AvaI 4477 CCCGAG C^[CT]CG[GA]G
BcgI 3683 GCAAGGAAGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BlpI 2146 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 2146 GCTGAGC GC^T[ACGT]AGC
BsaHI 3556 GGCGTC G[GA]^CG[CT]C
BsaWI 2923 ACCGGA [AT]^CCGG[AT]
BsiEI 1030 CGACCG CG[GA][CT]^CG
BsiHKAI 4146 GAGCAC G[AT]GC[AT]^C
BsiWI 3954 CGTACG C^GTACG
BsoBI 4477 CCCGAG C^[CT]CG[GA]G
BspHI 981 TCATGA T^CATGA
BspLU11I 3109 ACATGT A^CATGT
BsrBI 2149 GAGCGG CCGCTC(-3/-3)
BstEII 1109 GGTCACC G^GT[ACGT]ACC
BstPI 1109 GGTCACC G^GT[ACGT]ACC
Bsu36I 3172 CCTGAGG CC^T[ACGT]AGG
CelII 2146 GCTGAGC GC^T[ACGT]AGC
CpoI 504 CGGACCG CG^G[AT]CCG
DraIII 3137 CACCAGGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 3550 GACTGTGGCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 3172 CCTGAGG CC^T[ACGT]AGG
EcoO65I 1109 GGTCACC G^GT[ACGT]ACC
EcoRI 1378 GAATTC G^AATTC
EcoRV 5664 GATATC GAT^ATC
EcoT22I 1091 ATGCAT ATGCA^T
Hin1I 3556 GGCGTC G[GA]^CG[CT]C
HpaI 4590 GTTAAC GTT^AAC
NaeI 2276 GCCGGC GCC^GGC
NgoMIV 2276 GCCGGC G^CCGGC
NheI 2167 GCTAGC G^CTAGC
NsiI 1091 ATGCAT ATGCA^T
PshAI 3859 GACCAAAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Psp1406I 1569 AACGTT AA^CGTT
RcaI 981 TCATGA T^CATGA
RsrII 504 CGGACCG CG^G[AT]CCG
SalI 3559 GTCGAC G^TCGAC
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
Acc65I 1277 GGTACC G^GTACC
5552 GGTACC
AccII 472 CGCG CG^CG
2884 CGCG
AflII 783 CTTAAG C^TTAAG
5871 CTTAAG
Asp718I 1277 GGTACC G^GTACC
5552 GGTACC
BalI 2866 TGGCCA TGG^CCA
3945 TGGCCA
BamHI 1081 GGATCC G^GATCC
6471 GGATCC
BanII 2596 GAGCCC G[GA]GC[CT]^C
3586 GAGCCC
BciVI 1645 GGATAC GTATCC(6/5)
558 GTATCC
BcnI 2268 CCCGG CC^[GC]GG
5275 CCCGG
BfrI 783 CTTAAG C^TTAAG
5871 CTTAAG
BsrFI 1274 GCCGGT [GA]^CCGG[CT]
2276 GCCGGC
BstUI 472 CGCG CG^CG
2884 CGCG
BstXI 4457 CCATGCAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
6160 CCAAATACTTGG
Cfr10I 1274 GCCGGT [GA]^CCGG[CT]
2276 GCCGGC
EcoNI 1650 CCTCTCTAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
5884 CCTTGATTAGG
KpnI 1277 GGTACC GGTAC^C
5552 GGTACC
MfeI 341 CAATTG C^AATTG
5435 CAATTG
MluNI 2866 TGGCCA TGG^CCA
3945 TGGCCA
MscI 2866 TGGCCA TGG^CCA
3945 TGGCCA
MunI 341 CAATTG C^AATTG
5435 CAATTG
MvnI 472 CGCG CG^CG
2884 CGCG
NciI 2268 CCCGG CC^[GC]GG
5275 CCCGG
NdeI 886 CATATG CA^TATG
5219 CATATG
SexAI 1318 ACCTGGT A^CC[AT]GGT
3138 ACCAGGT
SphI 4322 GCATGC GCATG^C
5794 GCATGC
XbaI 283 TCTAGA T^CTAGA
1765 TCTAGA
#########################################
# 3 OR MORE CUT ENZYMES ( 118 enzymes) #
#########################################
AccI AciI AcsI AfaI AluI
AlwI AlwNI ApoI Asp700I AvaII
BanI BbsI BbvI BclI BfaI
BmyI BpmI BpuAI BsaI BsaJI
BseRI BsgI BsiYI BslI BsmAI
BsmBI BsmFI BsmI Bsp1286I Bsp1407I
BspMI BsrDI BsrGI BsrI BssKI
BstAPI BstF5I BstNI BstYI Cac8I
CfoI Cfr13I DdeI DpnII DraI
DraII DsaI EaeI EarI EcoO109I
EcoRII EcoT14I FauI FbaI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI HinP1I HincII HindII HindIII
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MnlI MseI MslI MspA1I
MspI MvaI MwoI NcoI NdeII
NlaIII NlaIV NspI PflMI PleI
PpuMI PstI PvuII RsaI SapI
Sau3AI Sau96I ScaI ScrFI SfaNI
SfcI SmlI SspBI SspI StuI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I Van91I
XcmI XhoII XmnI