##################################
# NO CUT ENZYMES ( 60 enzymes) #
##################################
AatII Acc65I AccIII AclI AgeI
AlwNI Aor51HI AscI Asp718I BbrPI
BcgI BciVI BglII BlpI Bpu1102I
BsaAI BseAI BsiWI BsmI BspEI
BspLU11I BssHII Bst1107I BstZ17I CelII
CpoI DraIII DrdI Eco47III EcoRI
EcoRV EcoT22I FseI HpaI KpnI
KspI MfeI MroI MunI NcoI
NheI NotI NruI NsiI PacI
PinAI PmaCI PmeI PmlI Psp1406I
RsrII SacII SbfI SfiI SgrAI
SnaBI SpeI Sse8387I SspI SwaI
###################################
# ONE CUT ENZYMES ( 41 enzymes) #
###################################
AflII 2479 CTTAAG C^TTAAG
AflIII 591 ACGCGT A^C[GA][CT]GT
ApaI 1237 GGGCCC GGGCC^C
Asp700I 3121 GAAGAAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AviII 1523 TGCGCA TGC^GCA
AvrII 1072 CCTAGG C^CTAGG
BbvCI 2513 CCTCAGC CCTCAGC(-5/-2)
BfrI 2479 CTTAAG C^TTAAG
BglI 1299 GCCTTATGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlnI 1072 CCTAGG C^CTAGG
BsgI 5095 CTGCAC GTGCAG(16/14)
BsmBI 573 CGTCTC CGTCTC(1/5)
BspDI 3218 ATCGAT AT^CGAT
BspHI 1418 TCATGA T^CATGA
BstAPI 1942 GCACTGCATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 1844 TTCGAA TT^CGAA
ClaI 3218 ATCGAT AT^CGAT
EagI 663 CGGCCG C^GGCCG
EclXI 663 CGGCCG C^GGCCG
Eco52I 663 CGGCCG C^GGCCG
FspI 1523 TGCGCA TGC^GCA
HincII 486 GTCGAC GT[CT]^[GA]AC
HindII 486 GTCGAC GT[CT]^[GA]AC
MluI 591 ACGCGT A^CGCGT
NaeI 650 GCCGGC GCC^GGC
NgoMIV 650 GCCGGC G^CCGGC
NspV 1844 TTCGAA TT^CGAA
PaeR7I 2423 CTCGAG C^TCGAG
PflMI 990 CCAGACTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PvuI 3220 CGATCG CGAT^CG
RcaI 1418 TCATGA T^CATGA
SalI 486 GTCGAC G^TCGAC
SapI 3778 GCTCTTC GCTCTTC(1/4)
SexAI 1032 ACCTGGT A^CC[AT]GGT
SfuI 1844 TTCGAA TT^CGAA
SphI 1947 GCATGC GCATG^C
StuI 4241 AGGCCT AGG^CCT
Van91I 990 CCAGACTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 316 TCTAGA T^CTAGA
XhoI 2423 CTCGAG C^TCGAG
XmnI 3121 GAAGAAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 46 enzymes) #
###################################
AccI 486 GTCGAC GT^[AC][GT]AC
1220 GTAGAC
AcyI 566 GGCGCC G[GA]^CG[CT]C
1412 GGCGCC
AhdI 993 GACTTTGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2553 GACGTATGGTC
Alw44I 1735 GTGCAC G^TGCAC
4958 GTGCAC
ApaLI 1735 GTGCAC G^TGCAC
4958 GTGCAC
AseI 2700 ATTAAT AT^TAAT
4768 ATTAAT
AsnI 2700 ATTAAT AT^TAAT
4768 ATTAAT
AspEI 993 GACTTTGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2553 GACGTATGGTC
AspI 1031 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
2782 GACACTGTC
BalI 629 TGGCCA TGG^CCA
4057 TGGCCA
BamHI 810 GGATCC G^GATCC
2376 GGATCC
BbeI 566 GGCGCC GGCGC^C
1412 GGCGCC
BclI 1967 TGATCA T^GATCA
2885 TGATCA
BsaHI 566 GGCGCC G[GA]^CG[CT]C
1412 GGCGCC
BsaWI 1882 ACCGGA [AT]^CCGG[AT]
3086 ACCGGA
Bsp1407I 4174 TGTACA T^GTACA
4293 TGTACA
BspMI 1631 ACCTGC ACCTGC(4/8)
4776 GCAGGT
BsrFI 650 GCCGGC [GA]^CCGG[CT]
665 GCCGGT
BsrGI 4174 TGTACA T^GTACA
4293 TGTACA
BstEII 168 GGTAACC G^GT[ACGT]ACC
1164 GGTGACC
BstPI 168 GGTAACC G^GT[ACGT]ACC
1164 GGTGACC
BstXI 494 CCAGCATCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4887 CCATGCAACTGG
Cfr10I 650 GCCGGC [GA]^CCGG[CT]
665 GCCGGT
DraI 5376 TTTAAA TTT^AAA
5412 TTTAAA
Eam1105I 993 GACTTTGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2553 GACGTATGGTC
EcoNI 3843 CCTGGCAAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
4151 CCTCTAGGAGG
EcoO65I 168 GGTAACC G^GT[ACGT]ACC
1164 GGTGACC
FbaI 1967 TGATCA T^GATCA
2885 TGATCA
HgaI 593 GCGTC GACGC(5/10)
2583 GCGTC
Hin1I 566 GGCGCC G[GA]^CG[CT]C
1412 GGCGCC
KasI 566 GGCGCC G^GCGCC
1412 GGCGCC
MluNI 629 TGGCCA TGG^CCA
4057 TGGCCA
MscI 629 TGGCCA TGG^CCA
4057 TGGCCA
NarI 566 GGCGCC GG^CGCC
1412 GGCGCC
NdeI 3056 CATATG CA^TATG
4579 CATATG
PflFI 1031 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
2782 GACACTGTC
PshAI 879 GACCACGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
1402 GACCCTCGTC
PshBI 2700 ATTAAT AT^TAAT
4768 ATTAAT
PstI 3554 CTGCAG CTGCA^G
4414 CTGCAG
ScaI 1698 AGTACT AGT^ACT
3067 AGTACT
SfoI 566 GGCGCC GGC^GCC
1412 GGCGCC
SmaI 545 CCCGGG CCC^GGG
1160 CCCGGG
SspBI 4174 TGTACA T^GTACA
4293 TGTACA
Tth111I 1031 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
2782 GACACTGTC
XmaCI 545 CCCGGG C^CCGGG
1160 CCCGGG
XmaI 545 CCCGGG C^CCGGG
1160 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 111 enzymes) #
#########################################
AccII AciI AcsI AfaI AluI
AlwI AocI ApoI AspHI AvaI
AvaII BanI BanII BbsI BbvI
BcnI BfaI BmyI BpmI BpuAI
BsaBI BsaI BsaJI BseRI BsiEI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BsrBI BsrDI BsrI
BssKI BssSI BstF5I BstNI BstUI
BstYI Bsu36I Cac8I CfoI Cfr13I
DdeI DpnII DraII DsaI EaeI
EarI Eco81I EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HhaI HinP1I HindIII HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MamI MboI MboII
MflI MnlI MseI MslI MspA1I
MspI MvaI MvnI MwoI NciI
NdeII NlaIII NlaIV NspI PleI
PpuMI PvuII RsaI SacI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I XcmI
XhoII