##################################
# NO CUT ENZYMES ( 66 enzymes) #
##################################
AatII AclI AflII AgeI Aor51HI
AscI AspI AviII BcgI BclI
BfrI BsiEI BsiWI BspDI BspHI
BssHII Bst1107I BstBI BstEII BstPI
BstZ17I ClaI CpoI DraIII EagI
EclXI Eco47III Eco52I EcoO65I EcoRV
FbaI FseI FspI HpaI KspI
MfeI MluI MunI NaeI NgoMIV
NheI NotI NruI NspV PacI
PflFI PinAI PmeI Psp1406I PvuI
RcaI RsrII SacII SalI SbfI
SexAI SfiI SfuI SgrAI SnaBI
SpeI Sse8387I SspI SwaI Tth111I
XbaI
###################################
# ONE CUT ENZYMES ( 43 enzymes) #
###################################
AccIII 4019 TCCGGA T^CCGGA
Alw44I 1019 GTGCAC G^TGCAC
ApaLI 1019 GTGCAC G^TGCAC
AseI 3106 ATTAAT AT^TAAT
AsnI 3106 ATTAAT AT^TAAT
BamHI 1290 GGATCC G^GATCC
BbeI 5373 GGCGCC GGCGC^C
BbrPI 1565 CACGTG CAC^GTG
BciVI 2367 GGATAC GTATCC(6/5)
BglI 927 GCCATTCCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 1163 AGATCT A^GATCT
BsaAI 1565 CACGTG [CT]AC^GT[GA]
BsaBI 2760 GATGCCTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseAI 4019 TCCGGA T^CCGGA
BsmBI 2616 CGTCTC CGTCTC(1/5)
Bsp1407I 2075 TGTACA T^GTACA
BspEI 4019 TCCGGA T^CCGGA
BspLU11I 1623 ACATGT A^CATGT
BsrBI 4319 CCGCTC CCGCTC(-3/-3)
BsrFI 1373 ACCGGC [GA]^CCGG[CT]
BsrGI 2075 TGTACA T^GTACA
Cfr10I 1373 ACCGGC [GA]^CCGG[CT]
EcoRI 3988 GAATTC G^AATTC
EcoT22I 2722 ATGCAT ATGCA^T
HindIII 2046 AAGCTT A^AGCTT
KasI 5373 GGCGCC G^GCGCC
MaeII 1566 ACGT A^CGT
MamI 2760 GATGCCTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI 4019 TCCGGA T^CCGGA
NarI 5373 GGCGCC GG^CGCC
NsiI 2722 ATGCAT ATGCA^T
PmaCI 1565 CACGTG CAC^GTG
PmlI 1565 CACGTG CAC^GTG
PshBI 3106 ATTAAT AT^TAAT
SapI 1083 GCTCTTC GCTCTTC(1/4)
ScaI 3518 AGTACT AGT^ACT
SfoI 5373 GGCGCC GGC^GCC
SmaI 3233 CCCGGG CCC^GGG
SphI 4124 GCATGC GCATG^C
SspBI 2075 TGTACA T^GTACA
StuI 2927 AGGCCT AGG^CCT
XmaCI 3233 CCCGGG C^CCGGG
XmaI 3233 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 20 enzymes) #
###################################
Acc65I 456 GGTACC G^GTACC
5267 GGTACC
AcyI 2614 GGCGTC G[GA]^CG[CT]C
5373 GGCGCC
AhdI 1470 GACCGGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
5123 GACCTTGAGTC
Asp700I 2043 GAAAAGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3546 GAACATTTTC
Asp718I 456 GGTACC G^GTACC
5267 GGTACC
AspEI 1470 GACCGGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
5123 GACCTTGAGTC
AvrII 4579 CCTAGG C^CTAGG
4590 CCTAGG
BlnI 4579 CCTAGG C^CTAGG
4590 CCTAGG
BsaHI 2614 GGCGTC G[GA]^CG[CT]C
5373 GGCGCC
BsaWI 3536 ACCGGA [AT]^CCGG[AT]
4019 TCCGGA
BsmI 863 GCATTC GAATGC(1/-1)
1844 GCATTC
BsrDI 1024 CATTGC GCAATG(2/0)
1355 GCAATG
DraI 4838 TTTAAA TTT^AAA
5349 TTTAAA
DrdI 292 GACCCTGGAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1774 GACCGCACAGTC
Eam1105I 1470 GACCGGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
5123 GACCTTGAGTC
HgaI 1300 GACGC GACGC(5/10)
2615 GCGTC
Hin1I 2614 GGCGTC G[GA]^CG[CT]C
5373 GGCGCC
KpnI 456 GGTACC GGTAC^C
5267 GGTACC
NdeI 1317 CATATG CA^TATG
3281 CATATG
XmnI 2043 GAAAAGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3546 GAACATTTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 129 enzymes) #
#########################################
AccI AccII AciI AcsI AfaI
AflIII AluI AlwI AlwNI AocI
ApaI ApoI AspHI AvaI AvaII
BalI BanI BanII BbsI BbvCI
BbvI BcnI BfaI BlpI BmyI
BpmI Bpu1102I BpuAI BsaI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BspMI
BsrI BssKI BssSI BstAPI BstF5I
BstNI BstUI BstXI BstYI Bsu36I
Cac8I CelII CfoI Cfr13I DdeI
DpnII DraII DsaI EaeI EarI
Eco81I EcoNI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HhaI HinP1I HincII HindII
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeIII MboI MboII MflI
MluNI MnlI MscI MseI MslI
MspA1I MspI MvaI MvnI MwoI
NciI NcoI NdeII NlaIII NlaIV
NspI PaeR7I PflMI PleI PpuMI
PshAI PstI PvuII RsaI SacI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
Van91I XcmI XhoI XhoII