##################################
# NO CUT ENZYMES ( 71 enzymes) #
##################################
AatII AccII AccIII AclI AgeI
AscI AspI AviII AvrII BbeI
BbrPI BclI BlnI BsaAI BsaWI
BseAI BsiWI BsmI BspDI BspEI
BssHII Bst1107I BstEII BstPI BstUI
BstZ17I ClaI CpoI DraIII DrdI
EagI EarI EclXI Eco52I EcoO65I
EcoRV EcoT22I FbaI FseI FspI
HpaI KasI Ksp632I KspI MluI
MroI MvnI NarI NotI NruI
NsiI PflFI PinAI PmaCI PmeI
PmlI PshAI Psp1406I PvuI RsrII
SacII SapI SbfI SfiI SfoI
SgrAI SnaBI SpeI Sse8387I SwaI
Tth111I
###################################
# ONE CUT ENZYMES ( 42 enzymes) #
###################################
AflII 3568 CTTAAG C^TTAAG
AhdI 3219 GACATTGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 747 GTGCAC G^TGCAC
Aor51HI 2322 AGCGCT AGC^GCT
ApaI 211 GGGCCC GGGCC^C
ApaLI 747 GTGCAC G^TGCAC
AspEI 3219 GACATTGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BamHI 187 GGATCC G^GATCC
BcgI 2432 GCACCCAACTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfrI 3568 CTTAAG C^TTAAG
BglII 5347 AGATCT A^GATCT
BsaBI 433 GATGAATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 1030 GAGACC GGTCTC(1/5)
BsiEI 4083 CGGTCG CG[GA][CT]^CG
BsmBI 564 GAGACG CGTCTC(1/5)
BspLU11I 3821 ACATGT A^CATGT
BsrDI 72 CATTGC GCAATG(2/0)
BsrFI 866 GCCGGC [GA]^CCGG[CT]
BssSI 2440 CTCGTG CACGAG(-5/-1)
BstAPI 4275 GCAGAGGTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 2750 TTCGAA TT^CGAA
Cfr10I 866 GCCGGC [GA]^CCGG[CT]
Eam1105I 3219 GACATTGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 2322 AGCGCT AGC^GCT
MaeII 4990 ACGT A^CGT
MamI 433 GATGAATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NaeI 866 GCCGGC GCC^GGC
NcoI 2909 CCATGG C^CATGG
NgoMIV 866 GCCGGC G^CCGGC
NheI 993 GCTAGC G^CTAGC
NspV 2750 TTCGAA TT^CGAA
PacI 4518 TTAATTAA TTAAT^TAA
PaeR7I 3326 CTCGAG C^TCGAG
SalI 1072 GTCGAC G^TCGAC
SexAI 1117 ACCTGGT A^CC[AT]GGT
SfuI 2750 TTCGAA TT^CGAA
SmaI 692 CCCGGG CCC^GGG
SphI 3657 GCATGC GCATG^C
XbaI 1974 TCTAGA T^CTAGA
XhoI 3326 CTCGAG C^TCGAG
XmaCI 692 CCCGGG C^CCGGG
XmaI 692 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
Acc65I 1090 GGTACC G^GTACC
1213 GGTACC
AflIII 3821 ACATGT A^C[GA][CT]GT
4990 ACGTGT
AseI 4521 ATTAAT AT^TAAT
5338 ATTAAT
AsnI 4521 ATTAAT AT^TAAT
5338 ATTAAT
Asp718I 1090 GGTACC G^GTACC
1213 GGTACC
AvaI 692 CCCGGG C^[CT]CG[GA]G
3326 CTCGAG
BbvCI 382 CCTCAGC CCTCAGC(-5/-2)
1578 CCTCAGC
BciVI 617 GTATCC GTATCC(6/5)
1122 GTATCC
BglI 204 GCCTGGAGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1281 GCCCAGGCGGC
BsoBI 692 CCCGGG C^[CT]CG[GA]G
3326 CTCGAG
BspHI 854 TCATGA T^CATGA
2072 TCATGA
BsrBI 305 CCGCTC CCGCTC(-3/-3)
1344 CCGCTC
EcoRI 784 GAATTC G^AATTC
3835 GAATTC
FauI 57 GCGGG CCCGC(4/6)
244 GCGGG
HaeII 1159 GGCGCT [GA]GCGC^[CT]
2322 AGCGCT
KpnI 1090 GGTACC GGTAC^C
1213 GGTACC
MfeI 548 CAATTG C^AATTG
3310 CAATTG
MunI 548 CAATTG C^AATTG
3310 CAATTG
NdeI 3302 CATATG CA^TATG
4062 CATATG
NspI 3657 GCATGC [GA]CATG^[CT]
3821 ACATGT
PflMI 2517 CCAAGTGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2766 CCAAATTCTGG
PpuMI 4 GGGTCCC [GA]G^G[AT]CC[CT]
4808 AGGTCCC
PshBI 4521 ATTAAT AT^TAAT
5338 ATTAAT
RcaI 854 TCATGA T^CATGA
2072 TCATGA
SspI 5020 AATATT AAT^ATT
5148 AATATT
StuI 1817 AGGCCT AGG^CCT
3107 AGGCCT
Van91I 2517 CCAAGTGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2766 CCAAATTCTGG
XcmI 1634 CCAGGAACTCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
2975 CCAGCAACCTCCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 117 enzymes) #
#########################################
AccI AciI AcsI AcyI AfaI
AluI AlwI AlwNI AocI ApoI
Asp700I AspHI AvaII BalI BanI
BanII BbsI BbvI BcnI BfaI
BlpI BmyI BpmI Bpu1102I BpuAI
BsaHI BsaJI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI Bsp1286I
Bsp1407I BspMI BsrGI BsrI BssKI
BstF5I BstNI BstXI BstYI Bsu36I
Cac8I CelII CfoI Cfr13I DdeI
DpnII DraI DraII DsaI EaeI
Eco81I EcoNI EcoO109I EcoRII EcoT14I
Fnu4HI FokI HaeIII HapII HgaI
HhaI Hin1I HinP1I HincII HindII
HindIII HinfI HpaII HphI ItaI
MaeI MaeIII MboI MboII MflI
MluNI MnlI MscI MseI MslI
MspA1I MspI MvaI MwoI NciI
NdeII NlaIII NlaIV PleI PstI
PvuII RsaI SacI Sau3AI Sau96I
ScaI ScrFI SfaNI SfcI SmlI
SspBI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
XhoII XmnI