##################################
# NO CUT ENZYMES ( 67 enzymes) #
##################################
Acc65I AccIII AclI AgeI AscI
AseI AsnI Asp718I AspI AviII
AvrII BbeI BbrPI BlnI BsaWI
BseAI BsiEI BsiWI BspEI BssHII
BstBI CpoI DrdI EagI EclXI
Eco52I EcoRV EcoT22I FseI FspI
HincII HindII HpaI KasI KpnI
KspI MroI NarI NotI NruI
NsiI NspV PacI PflFI PinAI
PmaCI PmeI PmlI PshBI Psp1406I
PvuI RsrII SacII SalI SbfI
SexAI SfiI SfoI SfuI SgrAI
SnaBI SpeI SphI Sse8387I SwaI
Tth111I XbaI
###################################
# ONE CUT ENZYMES ( 63 enzymes) #
###################################
AatII 2333 GACGTC GACGT^C
AcyI 2333 GACGTC G[GA]^CG[CT]C
AflII 1931 CTTAAG C^TTAAG
AhdI 3512 GACCACCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 916 GTGCAC G^TGCAC
Aor51HI 2697 AGCGCT AGC^GCT
ApaI 3040 GGGCCC GGGCC^C
ApaLI 916 GTGCAC G^TGCAC
AspEI 3512 GACCACCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BalI 3286 TGGCCA TGG^CCA
BamHI 599 GGATCC G^GATCC
BbsI 1430 GTCTTC GAAGAC(2/6)
BbvCI 1224 CCTCAGC CCTCAGC(-5/-2)
BcgI 2417 CGAGTCTCCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 4168 GGATAC GTATCC(6/5)
BclI 549 TGATCA T^GATCA
BfrI 1931 CTTAAG C^TTAAG
BglII 721 AGATCT A^GATCT
BpuAI 1430 GTCTTC GAAGAC(2/6)
BsaBI 2224 GATTGGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 2333 GACGTC G[GA]^CG[CT]C
BsmBI 476 GAGACG CGTCTC(1/5)
Bsp1407I 1653 TGTACA T^GTACA
BspDI 929 ATCGAT AT^CGAT
BspLU11I 2085 ACATGT A^CATGT
BsrBI 1159 CCGCTC CCGCTC(-3/-3)
BsrFI 996 GCCGGC [GA]^CCGG[CT]
BsrGI 1653 TGTACA T^GTACA
BssSI 2903 CTCGTG CACGAG(-5/-1)
Bst1107I 4517 GTATAC GTA^TAC
BstEII 2882 GGTCACC G^GT[ACGT]ACC
BstPI 2882 GGTCACC G^GT[ACGT]ACC
BstZ17I 4517 GTATAC GTA^TAC
Cfr10I 996 GCCGGC [GA]^CCGG[CT]
ClaI 929 ATCGAT AT^CGAT
DraIII 3454 CACCCCGTG CAC[ACGT][ACGT][ACGT]^GTG
Eam1105I 3512 GACCACCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 2697 AGCGCT AGC^GCT
EcoO65I 2882 GGTCACC G^GT[ACGT]ACC
EcoRI 2009 GAATTC G^AATTC
FbaI 549 TGATCA T^GATCA
HgaI 2061 GACGC GACGC(5/10)
Hin1I 2333 GACGTC G[GA]^CG[CT]C
MamI 2224 GATTGGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 816 CAATTG C^AATTG
MluI 3439 ACGCGT A^CGCGT
MluNI 3286 TGGCCA TGG^CCA
MscI 3286 TGGCCA TGG^CCA
MunI 816 CAATTG C^AATTG
NaeI 996 GCCGGC GCC^GGC
NdeI 386 CATATG CA^TATG
NgoMIV 996 GCCGGC G^CCGGC
NheI 2700 GCTAGC G^CTAGC
PaeR7I 2415 CTCGAG C^TCGAG
PpuMI 3629 GGGTCCC [GA]G^G[AT]CC[CT]
ScaI 654 AGTACT AGT^ACT
SmaI 367 CCCGGG CCC^GGG
SspBI 1653 TGTACA T^GTACA
SspI 491 AATATT AAT^ATT
XcmI 2193 CCAAAGTGGTCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 2415 CTCGAG C^TCGAG
XmaCI 367 CCCGGG C^CCGGG
XmaI 367 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AccII 2063 CGCG CG^CG
3440 CGCG
AlwNI 1227 CAGCCTCTG CAG[ACGT][ACGT][ACGT]^CTG
1696 CAGCACCTG
AvaI 367 CCCGGG C^[CT]CG[GA]G
2415 CTCGAG
BlpI 3031 GCTAAGC GC^T[ACGT]AGC
3151 GCTTAGC
Bpu1102I 3031 GCTAAGC GC^T[ACGT]AGC
3151 GCTTAGC
BsaAI 2309 TACGTG [CT]AC^GT[GA]
4051 CACGTA
BsoBI 367 CCCGGG C^[CT]CG[GA]G
2415 CTCGAG
BstUI 2063 CGCG CG^CG
3440 CGCG
CelII 3031 GCTAAGC GC^T[ACGT]AGC
3151 GCTTAGC
CfoI 1000 GCGC GCG^C
2698 GCGC
EaeI 1965 TGGCCG [CT]^GGCC[GA]
3286 TGGCCA
HaeII 999 GGCGCT [GA]GCGC^[CT]
2697 AGCGCT
HhaI 1000 GCGC GCG^C
2698 GCGC
HinP1I 1000 GCGC G^CGC
2698 GCGC
HindIII 284 AAGCTT A^AGCTT
3034 AAGCTT
MvnI 2063 CGCG CG^CG
3440 CGCG
PflMI 2655 CCAGGCTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3523 CCAGGCTGTGG
PshAI 1850 GACCTGTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
2329 GACCGACGTC
SapI 2261 GAAGAGC GCTCTTC(1/4)
2266 GCTCTTC
StuI 2478 AGGCCT AGG^CCT
3253 AGGCCT
Van91I 2655 CCAGGCTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3523 CCAGGCTGTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 107 enzymes) #
#########################################
AccI AciI AcsI AfaI AflIII
AluI AlwI AocI ApoI Asp700I
AspHI AvaII BanI BanII BbvI
BcnI BfaI BglI BmyI BpmI
BsaI BsaJI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI BsmI
Bsp1286I BspHI BspMI BsrDI BsrI
BssKI BstAPI BstF5I BstNI BstXI
BstYI Bsu36I Cac8I Cfr13I DdeI
DpnII DraI DraII DsaI EarI
Eco81I EcoNI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeIII HapII
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MnlI MseI MslI MspA1I
MspI MvaI MwoI NciI NcoI
NdeII NlaIII NlaIV NspI PleI
PstI PvuII RcaI RsaI SacI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
XhoII XmnI