##################################
# NO CUT ENZYMES ( 74 enzymes) #
##################################
AatII AccIII AclI AhdI Aor51HI
ApaI AscI AspEI AspI AviII
AvrII BbrPI BcgI BlnI BlpI
Bpu1102I BsaBI BsaI BseAI BsiEI
BsiWI BspDI BspEI BsrBI BssHII
Bst1107I BstZ17I CelII ClaI CpoI
DrdI EagI Eam1105I EclXI Eco47III
Eco52I EcoNI EcoRV EcoT22I FseI
FspI KspI MamI MluI MroI
NotI NruI NsiI PacI PaeR7I
PflFI PmaCI PmeI PmlI PshAI
Psp1406I PvuI RsrII SacII SalI
SapI SbfI SexAI SfiI SmaI
SnaBI SpeI SphI Sse8387I StuI
Tth111I XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 52 enzymes) #
###################################
Acc65I 1363 GGTACC G^GTACC
AccI 4970 GTAGAC GT^[AC][GT]AC
AflII 1040 CTTAAG C^TTAAG
AflIII 2286 ACATGT A^C[GA][CT]GT
AgeI 89 ACCGGT A^CCGGT
Alw44I 1099 GTGCAC G^TGCAC
AocI 3178 CCTGAGG CC^T[ACGT]AGG
ApaLI 1099 GTGCAC G^TGCAC
AseI 4119 ATTAAT AT^TAAT
AsnI 4119 ATTAAT AT^TAAT
Asp718I 1363 GGTACC G^GTACC
AvaI 2732 CCCGAG C^[CT]CG[GA]G
BbeI 163 GGCGCC GGCGC^C
BfrI 1040 CTTAAG C^TTAAG
BglI 3227 GCCGACCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 3867 AGATCT A^GATCT
BsaAI 3393 TACGTG [CT]AC^GT[GA]
BsmBI 1854 GAGACG CGTCTC(1/5)
BsoBI 2732 CCCGAG C^[CT]CG[GA]G
Bsp1407I 2289 TGTACA T^GTACA
BspLU11I 2286 ACATGT A^CATGT
BsrDI 1566 GCAATG GCAATG(2/0)
BsrGI 2289 TGTACA T^GTACA
BstAPI 1385 GCAAACAATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 2249 GGTTACC G^GT[ACGT]ACC
BstPI 2249 GGTTACC G^GT[ACGT]ACC
Bsu36I 3178 CCTGAGG CC^T[ACGT]AGG
DraII 3316 AGGTCCT [GA]G^G[ACGT]CC[CT]
DraIII 4662 CACAATGTG CAC[ACGT][ACGT][ACGT]^GTG
Eco81I 3178 CCTGAGG CC^T[ACGT]AGG
EcoO109I 3316 AGGTCCT [GA]G^G[ACGT]CC[CT]
EcoO65I 2249 GGTTACC G^GT[ACGT]ACC
HincII 2395 GTTAAC GT[CT]^[GA]AC
HindII 2395 GTTAAC GT[CT]^[GA]AC
HpaI 2395 GTTAAC GTT^AAC
KasI 163 GGCGCC G^GCGCC
KpnI 1363 GGTACC GGTAC^C
NaeI 602 GCCGGC GCC^GGC
NarI 163 GGCGCC GG^CGCC
NgoMIV 602 GCCGGC G^CCGGC
NheI 4657 GCTAGC G^CTAGC
PflMI 1912 CCAGTTTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI 89 ACCGGT A^CCGGT
PpuMI 3316 AGGTCCT [GA]G^G[AT]CC[CT]
PshBI 4119 ATTAAT AT^TAAT
SfoI 163 GGCGCC GGC^GCC
SgrAI 88 CACCGGTG C[GA]^CCGG[CT]G
SspBI 2289 TGTACA T^GTACA
SwaI 339 ATTTAAAT ATTT^AAAT
Tsp45I 93 GTGAC ^GT[GC]AC
Van91I 1912 CCAGTTTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 3780 CCATCCTACTTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 32 enzymes) #
###################################
AccII 27 CGCG CG^CG
66 CGCG
AcyI 163 GGCGCC G[GA]^CG[CT]C
1166 GGCGTC
BalI 2784 TGGCCA TGG^CCA
3285 TGGCCA
BamHI 124 GGATCC G^GATCC
2326 GGATCC
BbvCI 3578 CCTCAGC CCTCAGC(-5/-2)
2914 GCTGAGG
BciVI 4194 GTATCC GTATCC(6/5)
4630 GTATCC
BsaHI 163 GGCGCC G[GA]^CG[CT]C
1166 GGCGTC
BsaWI 89 ACCGGT [AT]^CCGG[AT]
3274 ACCGGA
BsgI 970 CTGCAC GTGCAG(16/14)
1172 CTGCAC
BsmFI 500 GGGAC GGGAC(10/14)
3162 GGGAC
BsmI 3928 GAATGC GAATGC(1/-1)
3078 GCATTC
BspHI 2272 TCATGA T^CATGA
2445 TCATGA
BspMI 71 ACCTGC ACCTGC(4/8)
1046 GCAGGT
BsrFI 89 ACCGGT [GA]^CCGG[CT]
602 GCCGGC
BssSI 2259 CACGAG CACGAG(-5/-1)
1395 CTCGTG
BstUI 27 CGCG CG^CG
66 CGCG
Cfr10I 89 ACCGGT [GA]^CCGG[CT]
602 GCCGGC
EaeI 2784 TGGCCA [CT]^GGCC[GA]
3285 TGGCCA
EcoRI 4573 GAATTC G^AATTC
4815 GAATTC
HaeII 163 GGCGCC [GA]GCGC^[CT]
3235 GGCGCT
HgaI 2809 GACGC GACGC(5/10)
1167 GCGTC
Hin1I 163 GGCGCC G[GA]^CG[CT]C
1166 GGCGTC
MfeI 228 CAATTG C^AATTG
525 CAATTG
MluNI 2784 TGGCCA TGG^CCA
3285 TGGCCA
MscI 2784 TGGCCA TGG^CCA
3285 TGGCCA
MunI 228 CAATTG C^AATTG
525 CAATTG
MvnI 27 CGCG CG^CG
66 CGCG
NdeI 402 CATATG CA^TATG
1943 CATATG
NspI 2286 ACATGT [GA]CATG^[CT]
2504 ACATGC
RcaI 2272 TCATGA T^CATGA
2445 TCATGA
ScaI 1521 AGTACT AGT^ACT
2117 AGTACT
XbaI 4569 TCTAGA T^CTAGA
4577 TCTAGA
#########################################
# 3 OR MORE CUT ENZYMES ( 100 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AlwNI ApoI Asp700I AspHI AvaII
BanI BanII BbsI BbvI BclI
BcnI BfaI BmyI BpmI BpuAI
BsaJI BseRI BsiHKAI BsiYI BslI
BsmAI Bsp1286I BsrI BssKI BstBI
BstF5I BstNI BstXI BstYI Cac8I
CfoI Cfr13I DdeI DpnII DraI
DsaI EarI EcoRII EcoT14I FauI
FbaI Fnu4HI FokI HaeIII HapII
HhaI HinP1I HindIII HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MnlI
MseI MslI MspA1I MspI MvaI
MwoI NciI NcoI NdeII NlaIII
NlaIV NspV PleI PstI PvuII
RsaI SacI Sau3AI Sau96I ScrFI
SfaNI SfcI SfuI SmlI SspI
StyI TaqI TfiI Tru9I TseI
Tsp509I TspRI TthHB8I XhoII XmnI