##################################
# NO CUT ENZYMES ( 46 enzymes) #
##################################
AatII AccIII AhdI AscI AspEI
AviII BseAI BsiEI BsiWI BspDI
BspEI BssHII Bst1107I BstZ17I ClaI
CpoI DraIII DrdI EagI Eam1105I
EclXI Eco52I EcoRV FseI FspI
HpaI KspI MluI MroI NdeI
NheI NotI NruI PacI PaeR7I
PvuI RsrII SacII SbfI ScaI
SfiI SnaBI SpeI Sse8387I SwaI
XhoI
###################################
# ONE CUT ENZYMES ( 32 enzymes) #
###################################
Acc65I 6770 GGTACC G^GTACC
AclI 3041 AACGTT AA^CGTT
AflII 4758 CTTAAG C^TTAAG
Aor51HI 2024 AGCGCT AGC^GCT
Asp718I 6770 GGTACC G^GTACC
AspI 5334 GACATCGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 6443 CCTAGG C^CTAGG
BciVI 320 GGATAC GTATCC(6/5)
BclI 6590 TGATCA T^GATCA
BfrI 4758 CTTAAG C^TTAAG
BlnI 6443 CCTAGG C^CTAGG
BstAPI 7387 GCATCACCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Eco47III 2024 AGCGCT AGC^GCT
EcoT22I 5255 ATGCAT ATGCA^T
FbaI 6590 TGATCA T^GATCA
KpnI 6770 GGTACC GGTAC^C
MfeI 5984 CAATTG C^AATTG
MunI 5984 CAATTG C^AATTG
NaeI 201 GCCGGC GCC^GGC
NgoMIV 201 GCCGGC G^CCGGC
NsiI 5255 ATGCAT ATGCA^T
PflFI 5334 GACATCGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmeI 5235 GTTTAAAC GTTT^AAAC
Psp1406I 3041 AACGTT AA^CGTT
SacI 295 GAGCTC GAGCT^C
SalI 3840 GTCGAC G^TCGAC
SexAI 3977 ACCTGGT A^CC[AT]GGT
SgrAI 385 CACCGGTG C[GA]^CCGG[CT]G
SphI 3772 GCATGC GCATG^C
SspI 3202 AATATT AAT^ATT
StuI 3097 AGGCCT AGG^CCT
Tth111I 5334 GACATCGTC GAC[ACGT]^[ACGT][ACGT]GTC
###################################
# TWO CUT ENZYMES ( 30 enzymes) #
###################################
AccII 1044 CGCG CG^CG
5917 CGCG
AgeI 386 ACCGGT A^CCGGT
3655 ACCGGT
Asp700I 5716 GAAGTATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
6965 GAAGCAATTC
BalI 2500 TGGCCA TGG^CCA
4950 TGGCCA
BamHI 407 GGATCC G^GATCC
6802 GGATCC
BbeI 2051 GGCGCC GGCGC^C
2964 GGCGCC
BbrPI 6289 CACGTG CAC^GTG
7204 CACGTG
Bsp1407I 2447 TGTACA T^GTACA
6285 TGTACA
BspLU11I 1230 ACATGT A^CATGT
2360 ACATGT
BsrGI 2447 TGTACA T^GTACA
6285 TGTACA
BssSI 4124 CACGAG CACGAG(-5/-1)
7557 CACGAG
BstUI 1044 CGCG CG^CG
5917 CGCG
EcoNI 2108 CCTCGGGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
5262 CCTTGTGTAGG
HgaI 7466 GACGC GACGC(5/10)
3416 GCGTC
KasI 2051 GGCGCC G^GCGCC
2964 GGCGCC
MluNI 2500 TGGCCA TGG^CCA
4950 TGGCCA
MscI 2500 TGGCCA TGG^CCA
4950 TGGCCA
MvnI 1044 CGCG CG^CG
5917 CGCG
NarI 2051 GGCGCC GG^CGCC
2964 GGCGCC
NcoI 1414 CCATGG C^CATGG
3136 CCATGG
PinAI 386 ACCGGT A^CCGGT
3655 ACCGGT
PmaCI 6289 CACGTG CAC^GTG
7204 CACGTG
PmlI 6289 CACGTG CAC^GTG
7204 CACGTG
PshAI 2434 GACTGCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
6912 GACTCTTGTC
SfoI 2051 GGCGCC GGC^GCC
2964 GGCGCC
SmaI 2315 CCCGGG CCC^GGG
6844 CCCGGG
SspBI 2447 TGTACA T^GTACA
6285 TGTACA
XmaCI 2315 CCCGGG C^CCGGG
6844 CCCGGG
XmaI 2315 CCCGGG C^CCGGG
6844 CCCGGG
XmnI 5716 GAAGTATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
6965 GAAGCAATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 150 enzymes) #
#########################################
AccI AciI AcsI AcyI AfaI
AflIII AluI Alw44I AlwI AlwNI
AocI ApaI ApaLI ApoI AseI
AsnI AspHI AvaI AvaII BanI
BanII BbsI BbvCI BbvI BcgI
BcnI BfaI BglI BglII BlpI
BmyI BpmI Bpu1102I BpuAI BsaAI
BsaBI BsaHI BsaI BsaJI BsaWI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmBI BsmFI BsmI BsoBI
Bsp1286I BspHI BspMI BsrBI BsrDI
BsrFI BsrI BssKI BstBI BstEII
BstF5I BstNI BstPI BstXI BstYI
Bsu36I Cac8I CelII CfoI Cfr10I
Cfr13I DdeI DpnII DraI DraII
DsaI EaeI EarI Eco81I EcoO109I
EcoO65I EcoRI EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HhaI Hin1I HinP1I HincII HindII
HindIII HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MamI
MboI MboII MflI MnlI MseI
MslI MspA1I MspI MvaI MwoI
NciI NdeII NlaIII NlaIV NspI
NspV PflMI PleI PpuMI PshBI
PstI PvuII RcaI RsaI SapI
Sau3AI Sau96I ScrFI SfaNI SfcI
SfuI SmlI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I Van91I XbaI XcmI XhoII