##################################
# NO CUT ENZYMES ( 68 enzymes) #
##################################
Acc65I AccIII AclI AflII Aor51HI
AscI Asp718I AspI AvaI AviII
AvrII BamHI BcgI BfrI BlnI
BlpI Bpu1102I BsaBI BseAI BsiWI
BsoBI Bsp1407I BspDI BspEI BsrGI
BssHII BssSI Bst1107I BstBI BstZ17I
CelII ClaI CpoI Eco47III EcoRI
EcoRV FseI FspI HgaI HpaI
KpnI MamI MfeI MluI MroI
MunI NotI NruI NspV PacI
PaeR7I PflFI PmeI Psp1406I PvuI
RsrII SexAI SfiI SfuI SgrAI
SmaI SpeI SspBI SspI Tth111I
XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 34 enzymes) #
###################################
AatII 3198 GACGTC GACGT^C
AccI 1003 GTCGAC GT^[AC][GT]AC
AgeI 4614 ACCGGT A^CCGGT
AhdI 1620 GACTTCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspEI 1620 GACTTCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BbeI 69 GGCGCC GGCGC^C
BbrPI 3270 CACGTG CAC^GTG
BciVI 1375 GTATCC GTATCC(6/5)
BsaWI 4614 ACCGGT [AT]^CCGG[AT]
BsmBI 2335 GAGACG CGTCTC(1/5)
DrdI 3243 GACTGGCCAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 1620 GACTTCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI 5977 CCTGTCACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
HaeII 69 GGCGCC [GA]GCGC^[CT]
KasI 69 GGCGCC G^GCGCC
KspI 23 CCGCGG CCGC^GG
NaeI 6 GCCGGC GCC^GGC
NarI 69 GGCGCC GG^CGCC
NdeI 632 CATATG CA^TATG
NgoMIV 6 GCCGGC G^CCGGC
NheI 1829 GCTAGC G^CTAGC
PinAI 4614 ACCGGT A^CCGGT
PmaCI 3270 CACGTG CAC^GTG
PmlI 3270 CACGTG CAC^GTG
SacII 23 CCGCGG CCGC^GG
SalI 1003 GTCGAC G^TCGAC
SbfI 2896 CCTGCAGG CCTGCA^GG
SfoI 69 GGCGCC GGC^GCC
SnaBI 3592 TACGTA TAC^GTA
Sse8387I 2896 CCTGCAGG CCTGCA^GG
StuI 987 AGGCCT AGG^CCT
SwaI 6287 ATTTAAAT ATTT^AAAT
XbaI 4079 TCTAGA T^CTAGA
XcmI 4354 CCAGAGTGCCCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AcyI 69 GGCGCC G[GA]^CG[CT]C
3198 GACGTC
Alw44I 5352 GTGCAC G^TGCAC
5905 GTGCAC
ApaI 1112 GGGCCC GGGCC^C
3055 GGGCCC
ApaLI 5352 GTGCAC G^TGCAC
5905 GTGCAC
AseI 3231 ATTAAT AT^TAAT
5206 ATTAAT
AsnI 3231 ATTAAT AT^TAAT
5206 ATTAAT
BglI 13 GCCCGCCCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
3848 GCCTTGTGGGC
BglII 1395 AGATCT A^GATCT
3794 AGATCT
BsaHI 69 GGCGCC G[GA]^CG[CT]C
3198 GACGTC
BspHI 906 TCATGA T^CATGA
1855 TCATGA
BstEII 1366 GGTGACC G^GT[ACGT]ACC
2101 GGTAACC
BstPI 1366 GGTGACC G^GT[ACGT]ACC
2101 GGTAACC
DraI 4447 TTTAAA TTT^AAA
6288 TTTAAA
EagI 8 CGGCCG C^GGCCG
20 CGGCCG
EclXI 8 CGGCCG C^GGCCG
20 CGGCCG
Eco52I 8 CGGCCG C^GGCCG
20 CGGCCG
EcoO65I 1366 GGTGACC G^GT[ACGT]ACC
2101 GGTAACC
EcoT22I 379 ATGCAT ATGCA^T
4400 ATGCAT
Hin1I 69 GGCGCC G[GA]^CG[CT]C
3198 GACGTC
HincII 1003 GTCGAC GT[CT]^[GA]AC
4277 GTTGAC
HindII 1003 GTCGAC GT[CT]^[GA]AC
4277 GTTGAC
HindIII 3527 AAGCTT A^AGCTT
4160 AAGCTT
NsiI 379 ATGCAT ATGCA^T
4400 ATGCAT
PshAI 1690 GACATCCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
3650 GACCTGTGTC
PshBI 3231 ATTAAT AT^TAAT
5206 ATTAAT
RcaI 906 TCATGA T^CATGA
1855 TCATGA
ScaI 521 AGTACT AGT^ACT
5043 AGTACT
#########################################
# 3 OR MORE CUT ENZYMES ( 129 enzymes) #
#########################################
AccII AciI AcsI AfaI AflIII
AluI AlwI AlwNI AocI ApoI
Asp700I AspHI AvaII BalI BanI
BanII BbsI BbvCI BbvI BclI
BcnI BfaI BmyI BpmI BpuAI
BsaAI BsaI BsaJI BseRI BsgI
BsiEI BsiHKAI BsiYI BslI BsmAI
BsmFI BsmI Bsp1286I BspLU11I BspMI
BsrBI BsrDI BsrFI BsrI BssKI
BstAPI BstF5I BstNI BstUI BstXI
BstYI Bsu36I Cac8I CfoI Cfr10I
Cfr13I DdeI DpnII DraII DraIII
DsaI EaeI EarI Eco81I EcoO109I
EcoRII EcoT14I FauI FbaI Fnu4HI
FokI HaeIII HapII HhaI HinP1I
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MluNI MnlI MscI MseI
MslI MspA1I MspI MvaI MvnI
MwoI NciI NcoI NdeII NlaIII
NlaIV NspI PflMI PleI PpuMI
PstI PvuII RsaI SacI SapI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI SphI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I Van91I XhoII XmnI