##################################
# NO CUT ENZYMES ( 82 enzymes) #
##################################
Acc65I AccII AccIII AclI AhdI
ApaI AscI Asp718I AspEI AviII
BamHI BbeI BbrPI BcgI BciVI
BclI BlpI Bpu1102I BsaAI BsaBI
BseAI BsiWI BsmBI BspEI BsrBI
BssHII BstAPI BstBI BstUI CelII
CpoI EagI Eam1105I EclXI Eco52I
EcoNI FbaI FseI FspI KasI
KpnI KspI MamI MluI MroI
MvnI NaeI NarI NgoMIV NheI
NotI NruI NspV PacI PaeR7I
PflMI PmaCI PmeI PmlI PpuMI
PshAI Psp1406I PvuI RsrII SacII
SalI SbfI ScaI SexAI SfiI
SfoI SfuI SgrAI SmaI SnaBI
Sse8387I Van91I XbaI XcmI XhoI
XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 59 enzymes) #
###################################
AatII 1513 GACGTC GACGT^C
AflII 4708 CTTAAG C^TTAAG
Alw44I 6266 GTGCAC G^TGCAC
AocI 931 CCTCAGG CC^T[ACGT]AGG
Aor51HI 134 AGCGCT AGC^GCT
ApaLI 6266 GTGCAC G^TGCAC
AspI 6158 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 1805 CCCGAG C^[CT]CG[GA]G
AvrII 3450 CCTAGG C^CTAGG
BalI 648 TGGCCA TGG^CCA
BbvCI 1567 GCTGAGG CCTCAGC(-5/-2)
BcnI 207 CCGGG CC^[GC]GG
BfrI 4708 CTTAAG C^TTAAG
BglI 8 GCCTGGCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 1409 AGATCT A^GATCT
BlnI 3450 CCTAGG C^CTAGG
BsaI 5772 GGTCTC GGTCTC(1/5)
BsiEI 732 CGACCG CG[GA][CT]^CG
BsoBI 1805 CCCGAG C^[CT]CG[GA]G
Bsp1407I 4087 TGTACA T^GTACA
BspDI 6146 ATCGAT AT^CGAT
BspHI 4698 TCATGA T^CATGA
BspMI 466 ACCTGC ACCTGC(4/8)
BsrGI 4087 TGTACA T^GTACA
BssSI 1442 CTCGTG CACGAG(-5/-1)
BstEII 2114 GGTTACC G^GT[ACGT]ACC
BstPI 2114 GGTTACC G^GT[ACGT]ACC
BstXI 897 CCATGCTTATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 931 CCTCAGG CC^T[ACGT]AGG
CfoI 135 GCGC GCG^C
ClaI 6146 ATCGAT AT^CGAT
DraIII 551 CACATTGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 244 GACGGGTATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 134 AGCGCT AGC^GCT
Eco81I 931 CCTCAGG CC^T[ACGT]AGG
EcoO65I 2114 GGTTACC G^GT[ACGT]ACC
EcoRI 2599 GAATTC G^AATTC
EcoRV 2325 GATATC GAT^ATC
EcoT22I 5555 ATGCAT ATGCA^T
HaeII 134 AGCGCT [GA]GCGC^[CT]
HgaI 660 GACGC GACGC(5/10)
HhaI 135 GCGC GCG^C
HinP1I 135 GCGC G^CGC
HpaI 4758 GTTAAC GTT^AAC
MfeI 6407 CAATTG C^AATTG
MluNI 648 TGGCCA TGG^CCA
MscI 648 TGGCCA TGG^CCA
MunI 6407 CAATTG C^AATTG
NciI 207 CCGGG CC^[GC]GG
NdeI 973 CATATG CA^TATG
NsiI 5555 ATGCAT ATGCA^T
PflFI 6158 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
RcaI 4698 TCATGA T^CATGA
SacI 1688 GAGCTC GAGCT^C
SapI 967 GCTCTTC GCTCTTC(1/4)
SpeI 2908 ACTAGT A^CTAGT
SspBI 4087 TGTACA T^GTACA
SwaI 2491 ATTTAAAT ATTT^AAAT
Tth111I 6158 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AccI 22 GTATAC GT^[AC][GT]AC
4562 GTATAC
AcyI 660 GACGCC G[GA]^CG[CT]C
1513 GACGTC
AgeI 77 ACCGGT A^CCGGT
6246 ACCGGT
Asp700I 2864 GAATTTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
5223 GAAGCGTTTC
AspHI 1688 GAGCTC G[AT]GC[AT]^C
6266 GTGCAC
AvaII 4033 GGACC G^G[AT]CC
6331 GGTCC
BanII 1688 GAGCTC G[GA]GC[CT]^C
3705 GAGCCC
BsaHI 660 GACGCC G[GA]^CG[CT]C
1513 GACGTC
BsaWI 77 ACCGGT [AT]^CCGG[AT]
6246 ACCGGT
BsiHKAI 1688 GAGCTC G[AT]GC[AT]^C
6266 GTGCAC
BsrFI 77 ACCGGT [GA]^CCGG[CT]
6246 ACCGGT
Bst1107I 22 GTATAC GTA^TAC
4562 GTATAC
BstZ17I 22 GTATAC GTA^TAC
4562 GTATAC
Cfr10I 77 ACCGGT [GA]^CCGG[CT]
6246 ACCGGT
FauI 161 CCCGC CCCGC(4/6)
375 GCGGG
Hin1I 660 GACGCC G[GA]^CG[CT]C
1513 GACGTC
HincII 1474 GTCAAC GT[CT]^[GA]AC
4758 GTTAAC
HindII 1474 GTCAAC GT[CT]^[GA]AC
4758 GTTAAC
NcoI 327 CCATGG C^CATGG
651 CCATGG
PinAI 77 ACCGGT A^CCGGT
6246 ACCGGT
PstI 1518 CTGCAG CTGCA^G
2438 CTGCAG
SphI 382 GCATGC GCATG^C
5682 GCATGC
StuI 981 AGGCCT AGG^CCT
3877 AGGCCT
XmnI 2864 GAATTTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
5223 GAAGCGTTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 93 enzymes) #
#########################################
AciI AcsI AfaI AflIII AluI
AlwI AlwNI ApoI AseI AsnI
BanI BbsI BbvI BfaI BmyI
BpmI BpuAI BsaJI BseRI BsgI
BsiYI BslI BsmAI BsmFI BsmI
Bsp1286I BspLU11I BsrDI BsrI BssKI
BstF5I BstNI BstYI Cac8I Cfr13I
DdeI DpnII DraI DraII DsaI
EaeI EarI EcoO109I EcoRII EcoT14I
Fnu4HI FokI HaeIII HapII HindIII
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MnlI MseI MslI MspA1I
MspI MvaI MwoI NdeII NlaIII
NlaIV NspI PleI PshBI PvuII
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI SspI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I XhoII