##################################
# NO CUT ENZYMES ( 40 enzymes) #
##################################
AccIII AclI AhdI AscI AseI
AsnI Asp700I AspEI AviII BseAI
BsiWI BspEI BssHII Bst1107I BstZ17I
CpoI Eam1105I EcoT22I FseI FspI
HpaI MroI NotI NruI NsiI
PacI PmeI PshBI Psp1406I PvuI
RsrII SalI SbfI SfiI SgrAI
SnaBI Sse8387I SwaI XbaI XmnI
###################################
# ONE CUT ENZYMES ( 35 enzymes) #
###################################
AatII 4739 GACGTC GACGT^C
Acc65I 2166 GGTACC G^GTACC
AflII 9200 CTTAAG C^TTAAG
AgeI 5995 ACCGGT A^CCGGT
Asp718I 2166 GGTACC G^GTACC
AspI 10158 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 5494 CCTAGG C^CTAGG
BbvCI 437 GCTGAGG CCTCAGC(-5/-2)
BfrI 9200 CTTAAG C^TTAAG
BlnI 5494 CCTAGG C^CTAGG
BsaBI 2833 GATGAACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BspDI 5442 ATCGAT AT^CGAT
BssSI 6800 CTCGTG CACGAG(-5/-1)
BstBI 4206 TTCGAA TT^CGAA
BstEII 5701 GGTAACC G^GT[ACGT]ACC
BstPI 5701 GGTAACC G^GT[ACGT]ACC
ClaI 5442 ATCGAT AT^CGAT
DraI 931 TTTAAA TTT^AAA
DrdI 2864 GACACTCTCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO65I 5701 GGTAACC G^GT[ACGT]ACC
EcoRV 9783 GATATC GAT^ATC
KpnI 2166 GGTACC GGTAC^C
MamI 2833 GATGAACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 10338 CAATTG C^AATTG
MluI 9748 ACGCGT A^CGCGT
MunI 10338 CAATTG C^AATTG
NdeI 8006 CATATG CA^TATG
NspV 4206 TTCGAA TT^CGAA
PflFI 10158 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PinAI 5995 ACCGGT A^CCGGT
ScaI 7026 AGTACT AGT^ACT
SfuI 4206 TTCGAA TT^CGAA
SpeI 8973 ACTAGT A^CTAGT
SspI 2088 AATATT AAT^ATT
Tth111I 10158 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
###################################
# TWO CUT ENZYMES ( 12 enzymes) #
###################################
AccI 6518 GTAGAC GT^[AC][GT]AC
10752 GTAGAC
Aor51HI 190 AGCGCT AGC^GCT
2133 AGCGCT
BamHI 2785 GGATCC G^GATCC
6163 GGATCC
BsrBI 10977 CCGCTC CCGCTC(-3/-3)
320 GAGCGG
Eco47III 190 AGCGCT AGC^GCT
2133 AGCGCT
HindIII 1943 AAGCTT A^AGCTT
10152 AAGCTT
NheI 6206 GCTAGC G^CTAGC
9494 GCTAGC
PshAI 3596 GACAGCAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
4520 GACCAGAGTC
SexAI 2301 ACCTGGT A^CC[AT]GGT
10159 ACCAGGT
SmaI 172 CCCGGG CCC^GGG
10381 CCCGGG
XmaCI 172 CCCGGG C^CCGGG
10381 CCCGGG
XmaI 172 CCCGGG C^CCGGG
10381 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 171 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AflIII AluI Alw44I AlwI AlwNI
AocI ApaI ApaLI ApoI AspHI
AvaI AvaII BalI BanI BanII
BbeI BbrPI BbsI BbvI BcgI
BciVI BclI BcnI BfaI BglI
BglII BlpI BmyI BpmI Bpu1102I
BpuAI BsaAI BsaHI BsaI BsaJI
BsaWI BseRI BsgI BsiEI BsiHKAI
BsiYI BslI BsmAI BsmBI BsmFI
BsmI BsoBI Bsp1286I Bsp1407I BspHI
BspLU11I BspMI BsrDI BsrFI BsrGI
BsrI BssKI BstAPI BstF5I BstNI
BstUI BstXI BstYI Bsu36I Cac8I
CelII CfoI Cfr10I Cfr13I DdeI
DpnII DraII DraIII DsaI EaeI
EagI EarI EclXI Eco52I Eco81I
EcoNI EcoO109I EcoRI EcoRII EcoT14I
FauI FbaI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HincII HindII HinfI HpaII
HphI ItaI KasI Ksp632I KspI
MaeI MaeII MaeIII MboI MboII
MflI MluNI MnlI MscI MseI
MslI MspA1I MspI MvaI MvnI
MwoI NaeI NarI NciI NcoI
NdeII NgoMIV NlaIII NlaIV NspI
PaeR7I PflMI PleI PmaCI PmlI
PpuMI PstI PvuII RcaI RsaI
SacI SacII SapI Sau3AI Sau96I
ScrFI SfaNI SfcI SfoI SmlI
SphI SspBI StuI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I Van91I XcmI XhoI
XhoII