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# NO CUT ENZYMES ( 48 enzymes) #
##################################
AclI AgeI AhdI AscI AspEI
AviII BciVI BlpI Bpu1102I BsiWI
BsmBI BspDI Bst1107I BstBI BstZ17I
CelII ClaI DrdI Eam1105I FseI
FspI MfeI MluI MunI NheI
NotI NruI NspV PacI PaeR7I
PinAI PmeI PshAI Psp1406I PvuI
SalI SbfI SfiI SfuI SgrAI
SmaI SnaBI Sse8387I SwaI XbaI
XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 50 enzymes) #
###################################
AatII 1640 GACGTC GACGT^C
AccIII 1718 TCCGGA T^CCGGA
AflII 4461 CTTAAG C^TTAAG
AflIII 5445 ACATGT A^C[GA][CT]GT
Aor51HI 3190 AGCGCT AGC^GCT
ApaI 25 GGGCCC GGGCC^C
AseI 3679 ATTAAT AT^TAAT
AsnI 3679 ATTAAT AT^TAAT
Asp700I 3685 GAATAAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI 1865 GACTCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 74 CTCGGG C^[CT]CG[GA]G
BamHI 1293 GGATCC G^GATCC
BbrPI 542 CACGTG CAC^GTG
BbvCI 1859 GCTGAGG CCTCAGC(-5/-2)
BfrI 4461 CTTAAG C^TTAAG
BsaAI 542 CACGTG [CT]AC^GT[GA]
BsaI 377 GAGACC GGTCTC(1/5)
BseAI 1718 TCCGGA T^CCGGA
BsoBI 74 CTCGGG C^[CT]CG[GA]G
BspEI 1718 TCCGGA T^CCGGA
BspLU11I 5445 ACATGT A^CATGT
BssSI 2336 CACGAG CACGAG(-5/-1)
BstEII 3726 GGTGACC G^GT[ACGT]ACC
BstPI 3726 GGTGACC G^GT[ACGT]ACC
CpoI 137 CGGACCG CG^G[AT]CCG
EagI 142 CGGCCG C^GGCCG
EclXI 142 CGGCCG C^GGCCG
Eco47III 3190 AGCGCT AGC^GCT
Eco52I 142 CGGCCG C^GGCCG
EcoNI 2572 CCTTGCCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 3726 GGTGACC G^GT[ACGT]ACC
EcoRV 5344 GATATC GAT^ATC
MroI 1718 TCCGGA T^CCGGA
NdeI 4621 CATATG CA^TATG
PflFI 1865 GACTCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 568 CCAGGCTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 542 CACGTG CAC^GTG
PmlI 542 CACGTG CAC^GTG
PshBI 3679 ATTAAT AT^TAAT
RsrII 137 CGGACCG CG^G[AT]CCG
SacI 3224 GAGCTC GAGCT^C
SapI 554 GAAGAGC GCTCTTC(1/4)
ScaI 5235 AGTACT AGT^ACT
SexAI 5087 ACCTGGT A^CC[AT]GGT
SpeI 5670 ACTAGT A^CTAGT
SphI 5406 GCATGC GCATG^C
StuI 2271 AGGCCT AGG^CCT
Tth111I 1865 GACTCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 568 CCAGGCTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmnI 3685 GAATAAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 32 enzymes) #
###################################
Acc65I 1358 GGTACC G^GTACC
3410 GGTACC
Alw44I 3341 GTGCAC G^TGCAC
5025 GTGCAC
AocI 1232 CCTCAGG CC^T[ACGT]AGG
1418 CCTGAGG
ApaLI 3341 GTGCAC G^TGCAC
5025 GTGCAC
Asp718I 1358 GGTACC G^GTACC
3410 GGTACC
AvrII 3146 CCTAGG C^CTAGG
5182 CCTAGG
BcgI 1891 CGACATGAATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
103 GCAGCGGACTCG
BclI 999 TGATCA T^GATCA
3249 TGATCA
BglI 231 GCCGGAGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
302 GCCCCTGCGGC
BglII 351 AGATCT A^GATCT
5423 AGATCT
BlnI 3146 CCTAGG C^CTAGG
5182 CCTAGG
BsaBI 3299 GATGGAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
5118 GATTCATATC
BsaWI 480 ACCGGA [AT]^CCGG[AT]
1718 TCCGGA
BsiEI 142 CGGCCG CG[GA][CT]^CG
267 CGGTCG
Bsp1407I 4183 TGTACA T^GTACA
5448 TGTACA
BsrGI 4183 TGTACA T^GTACA
5448 TGTACA
BstAPI 2567 GCAAACCTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
4206 GCAGACCATGC
Bsu36I 1232 CCTCAGG CC^T[ACGT]AGG
1418 CCTGAGG
Eco81I 1232 CCTCAGG CC^T[ACGT]AGG
1418 CCTGAGG
EcoRI 365 GAATTC G^AATTC
4194 GAATTC
EcoT22I 1545 ATGCAT ATGCA^T
3563 ATGCAT
FbaI 999 TGATCA T^GATCA
3249 TGATCA
HpaI 4701 GTTAAC GTT^AAC
5666 GTTAAC
KpnI 1358 GGTACC GGTAC^C
3410 GGTACC
MamI 3299 GATGGAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
5118 GATTCATATC
MslI 3293 CACATTGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
3556 CATTGCTATG
NaeI 55 GCCGGC GCC^GGC
2787 GCCGGC
NcoI 850 CCATGG C^CATGG
1354 CCATGG
NgoMIV 55 GCCGGC G^CCGGC
2787 GCCGGC
NsiI 1545 ATGCAT ATGCA^T
3563 ATGCAT
SspBI 4183 TGTACA T^GTACA
5448 TGTACA
XcmI 1664 CCAGATCCTACATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
2362 CCAAGCCTTATCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 128 enzymes) #
#########################################
AccI AccII AciI AcsI AcyI
AfaI AluI AlwI AlwNI ApoI
AspHI AvaII BalI BanI BanII
BbeI BbsI BbvI BcnI BfaI
BmyI BpmI BpuAI BsaHI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI BsmI Bsp1286I BspHI
BspMI BsrBI BsrDI BsrFI BsrI
BssHII BssKI BstF5I BstNI BstUI
BstXI BstYI Cac8I CfoI Cfr10I
Cfr13I DdeI DpnII DraI DraII
DraIII DsaI EaeI EarI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
Hin1I HinP1I HincII HindII HindIII
HinfI HpaII HphI ItaI KasI
Ksp632I KspI MaeI MaeII MaeIII
MboI MboII MflI MluNI MnlI
MscI MseI MspA1I MspI MvaI
MvnI MwoI NarI NciI NdeII
NlaIII NlaIV NspI PleI PpuMI
PstI PvuII RcaI RsaI SacII
Sau3AI Sau96I ScrFI SfaNI SfcI
SfoI SmlI SspI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I XhoII