##################################
# NO CUT ENZYMES ( 63 enzymes) #
##################################
Acc65I AccIII AgeI AscI AseI
AsnI Asp700I Asp718I AvrII BamHI
BbeI BcgI BlnI BsaBI BsaWI
BseAI BsiEI BsiWI BspEI BsrBI
BssHII Bst1107I BstZ17I CpoI EagI
EclXI Eco52I EcoRI FseI HpaI
KasI KpnI KspI MamI MfeI
MluI MroI MunI NaeI NarI
NdeI NgoMIV NotI NruI PacI
PinAI PmeI PshBI PvuI RsrII
SacII SalI SapI SfiI SfoI
SgrAI SmaI SnaBI SpeI StuI
XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 48 enzymes) #
###################################
AatII 3639 GACGTC GACGT^C
AccI 198 GTAGAC GT^[AC][GT]AC
AclI 4897 AACGTT AA^CGTT
AflII 3403 CTTAAG C^TTAAG
AocI 1664 CCTCAGG CC^T[ACGT]AGG
Aor51HI 4552 AGCGCT AGC^GCT
ApaI 576 GGGCCC GGGCC^C
AviII 1164 TGCGCA TGC^GCA
BbrPI 4142 CACGTG CAC^GTG
BciVI 1403 GTATCC GTATCC(6/5)
BclI 2409 TGATCA T^GATCA
BfrI 3403 CTTAAG C^TTAAG
BglII 4699 AGATCT A^GATCT
BsaAI 4142 CACGTG [CT]AC^GT[GA]
BsmBI 232 GAGACG CGTCTC(1/5)
Bsp1407I 3808 TGTACA T^GTACA
BspDI 4090 ATCGAT AT^CGAT
BsrDI 4028 CATTGC GCAATG(2/0)
BsrFI 2850 ACCGGC [GA]^CCGG[CT]
BsrGI 3808 TGTACA T^GTACA
BssSI 1781 CACGAG CACGAG(-5/-1)
BstAPI 6015 GCACAGAGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 4062 TTCGAA TT^CGAA
Bsu36I 1664 CCTCAGG CC^T[ACGT]AGG
Cfr10I 2850 ACCGGC [GA]^CCGG[CT]
ClaI 4090 ATCGAT AT^CGAT
Eco47III 4552 AGCGCT AGC^GCT
Eco81I 1664 CCTCAGG CC^T[ACGT]AGG
EcoNI 3582 CCTTTGGTAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FbaI 2409 TGATCA T^GATCA
FspI 1164 TGCGCA TGC^GCA
HincII 4620 GTTGAC GT[CT]^[GA]AC
HindII 4620 GTTGAC GT[CT]^[GA]AC
NcoI 5379 CCATGG C^CATGG
NspV 4062 TTCGAA TT^CGAA
PaeR7I 1588 CTCGAG C^TCGAG
PmaCI 4142 CACGTG CAC^GTG
PmlI 4142 CACGTG CAC^GTG
PshAI 3639 GACGTCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Psp1406I 4897 AACGTT AA^CGTT
SbfI 5672 CCTGCAGG CCTGCA^GG
SexAI 1811 ACCAGGT A^CC[AT]GGT
SfuI 4062 TTCGAA TT^CGAA
Sse8387I 5672 CCTGCAGG CCTGCA^GG
SspBI 3808 TGTACA T^GTACA
SwaI 6323 ATTTAAAT ATTT^AAAT
XbaI 4678 TCTAGA T^CTAGA
XhoI 1588 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AflIII 3018 ACATGT A^C[GA][CT]GT
3816 ACATGT
AhdI 4623 GACAATGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
6279 GACTACCCGTC
Alw44I 6013 GTGCAC G^TGCAC
6028 GTGCAC
ApaLI 6013 GTGCAC G^TGCAC
6028 GTGCAC
AspEI 4623 GACAATGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
6279 GACTACCCGTC
AspI 286 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
2524 GACGTTGTC
BsgI 1127 GTGCAG GTGCAG(16/14)
4145 GTGCAG
BsmI 2477 GCATTC GAATGC(1/-1)
5252 GCATTC
BspLU11I 3018 ACATGT A^CATGT
3816 ACATGT
BstEII 977 GGTCACC G^GT[ACGT]ACC
5433 GGTAACC
BstPI 977 GGTCACC G^GT[ACGT]ACC
5433 GGTAACC
DrdI 254 GACACCTCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
969 GACTCGGCGGTC
Eam1105I 4623 GACAATGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
6279 GACTACCCGTC
EcoO65I 977 GGTCACC G^GT[ACGT]ACC
5433 GGTAACC
EcoRV 4737 GATATC GAT^ATC
6227 GATATC
EcoT22I 1973 ATGCAT ATGCA^T
2225 ATGCAT
HindIII 1835 AAGCTT A^AGCTT
3919 AAGCTT
NheI 2632 GCTAGC G^CTAGC
4289 GCTAGC
NsiI 1973 ATGCAT ATGCA^T
2225 ATGCAT
PflFI 286 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
2524 GACGTTGTC
PflMI 758 CCACGGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2698 CCACACGGTGG
SacI 158 GAGCTC GAGCT^C
2195 GAGCTC
ScaI 307 AGTACT AGT^ACT
3325 AGTACT
Tth111I 286 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
2524 GACGTTGTC
Van91I 758 CCACGGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2698 CCACACGGTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 122 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AluI AlwI AlwNI ApoI AspHI
AvaI AvaII BalI BanI BanII
BbsI BbvCI BbvI BcnI BfaI
BglI BlpI BmyI BpmI Bpu1102I
BpuAI BsaHI BsaI BsaJI BseRI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BspHI BspMI BsrI
BssKI BstF5I BstNI BstUI BstXI
BstYI Cac8I CelII CfoI Cfr13I
DdeI DpnII DraI DraII DraIII
DsaI EaeI EarI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MluNI MnlI MscI
MseI MslI MspA1I MspI MvaI
MvnI MwoI NciI NdeII NlaIII
NlaIV NspI PleI PpuMI PstI
PvuII RcaI RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI SphI
SspI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
XcmI XhoII