##################################
# NO CUT ENZYMES ( 48 enzymes) #
##################################
AclI AcsI AflII ApoI AscI
AseI AsnI AvrII BamHI BclI
BfrI BlnI BsiWI BspDI BspHI
BssHII Bst1107I BstBI BstZ17I ClaI
CpoI DraI DrdI EcoRI FbaI
FseI HpaI MfeI MluI MunI
NdeI NheI NotI NruI NspV
PacI PmeI PshBI Psp1406I RcaI
RsrII SalI SfuI SspI SwaI
TfiI Tsp509I XbaI
###################################
# ONE CUT ENZYMES ( 45 enzymes) #
###################################
AatII 1376 GACGTC GACGT^C
Acc65I 2949 GGTACC G^GTACC
AccIII 1392 TCCGGA T^CCGGA
AgeI 5461 ACCGGT A^CCGGT
Asp700I 3236 GAAGGAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 2949 GGTACC G^GTACC
AspI 573 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BbrPI 3711 CACGTG CAC^GTG
BciVI 5645 GGATAC GTATCC(6/5)
BglII 2597 AGATCT A^GATCT
BseAI 1392 TCCGGA T^CCGGA
BsmI 3311 GCATTC GAATGC(1/-1)
Bsp1407I 181 TGTACA T^GTACA
BspEI 1392 TCCGGA T^CCGGA
BspLU11I 3187 ACATGT A^CATGT
BsrGI 181 TGTACA T^GTACA
BstAPI 725 GCACAAGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 3137 GGTGACC G^GT[ACGT]ACC
BstPI 3137 GGTGACC G^GT[ACGT]ACC
EcoO65I 3137 GGTGACC G^GT[ACGT]ACC
EcoRV 6212 GATATC GAT^ATC
EcoT22I 425 ATGCAT ATGCA^T
HindIII 4523 AAGCTT A^AGCTT
KpnI 2949 GGTACC GGTAC^C
KspI 3460 CCGCGG CCGC^GG
MroI 1392 TCCGGA T^CCGGA
NsiI 425 ATGCAT ATGCA^T
PaeR7I 4927 CTCGAG C^TCGAG
PflFI 573 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PinAI 5461 ACCGGT A^CCGGT
PmaCI 3711 CACGTG CAC^GTG
PmlI 3711 CACGTG CAC^GTG
PshAI 4597 GACCGCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuI 4252 CGATCG CGAT^CG
SacII 3460 CCGCGG CCGC^GG
SbfI 5540 CCTGCAGG CCTGCA^GG
SexAI 5116 ACCAGGT A^CC[AT]GGT
SnaBI 3762 TACGTA TAC^GTA
SpeI 4860 ACTAGT A^CTAGT
SphI 5797 GCATGC GCATG^C
Sse8387I 5540 CCTGCAGG CCTGCA^GG
SspBI 181 TGTACA T^GTACA
Tth111I 573 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoI 4927 CTCGAG C^TCGAG
XmnI 3236 GAAGGAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AflIII 3187 ACATGT A^C[GA][CT]GT
3710 ACACGT
AhdI 1562 GACGGGCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
5505 GACATCCTGTC
Aor51HI 4739 AGCGCT AGC^GCT
5551 AGCGCT
AspEI 1562 GACGGGCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
5505 GACATCCTGTC
BbeI 686 GGCGCC GGCGC^C
5706 GGCGCC
BbsI 1029 GAAGAC GAAGAC(2/6)
2812 GTCTTC
BcgI 1427 CGAGTTTGATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
3728 CGACTCCTGTGC
BpuAI 1029 GAAGAC GAAGAC(2/6)
2812 GTCTTC
BsaBI 699 GATGTGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2420 GATGGTGATC
BsrDI 1791 CATTGC GCAATG(2/0)
2000 CATTGC
DraIII 2294 CACACAGTG CAC[ACGT][ACGT][ACGT]^GTG
4533 CACGAGGTG
EagI 3040 CGGCCG C^GGCCG
4248 CGGCCG
Eam1105I 1562 GACGGGCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
5505 GACATCCTGTC
EclXI 3040 CGGCCG C^GGCCG
4248 CGGCCG
Eco47III 4739 AGCGCT AGC^GCT
5551 AGCGCT
Eco52I 3040 CGGCCG C^GGCCG
4248 CGGCCG
HincII 3612 GTTGAC GT[CT]^[GA]AC
3747 GTCAAC
HindII 3612 GTTGAC GT[CT]^[GA]AC
3747 GTCAAC
KasI 686 GGCGCC G^GCGCC
5706 GGCGCC
MamI 699 GATGTGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2420 GATGGTGATC
NarI 686 GGCGCC GG^CGCC
5706 GGCGCC
SapI 783 GAAGAGC GCTCTTC(1/4)
5324 GCTCTTC
SfiI 3159 GGCCGCAAGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
5765 GGCCACGGAGGCC
SfoI 686 GGCGCC GGC^GCC
5706 GGCGCC
SgrAI 9 CGCCGGCG C[GA]^CCGG[CT]G
691 CGCCGGTG
SmaI 1639 CCCGGG CCC^GGG
1972 CCCGGG
XmaCI 1639 CCCGGG C^CCGGG
1972 CCCGGG
XmaI 1639 CCCGGG C^CCGGG
1972 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 137 enzymes) #
#########################################
AccI AccII AciI AcyI AfaI
AluI Alw44I AlwI AlwNI AocI
ApaI ApaLI AspHI AvaI AvaII
AviII BalI BanI BanII BbvCI
BbvI BcnI BfaI BglI BlpI
BmyI BpmI Bpu1102I BsaAI BsaHI
BsaI BsaJI BsaWI BseRI BsgI
BsiEI BsiHKAI BsiYI BslI BsmAI
BsmBI BsmFI BsoBI Bsp1286I BspMI
BsrBI BsrFI BsrI BssKI BssSI
BstF5I BstNI BstUI BstXI BstYI
Bsu36I Cac8I CelII CfoI Cfr10I
Cfr13I DdeI DpnII DraII DsaI
EaeI EarI Eco81I EcoNI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
FspI HaeII HaeIII HapII HgaI
HhaI Hin1I HinP1I HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MluNI
MnlI MscI MseI MslI MspA1I
MspI MvaI MvnI MwoI NaeI
NciI NcoI NdeII NgoMIV NlaIII
NlaIV NspI PflMI PleI PpuMI
PstI PvuII RsaI SacI Sau3AI
Sau96I ScaI ScrFI SfaNI SfcI
SmlI StuI StyI TaqI Tru9I
TseI Tsp45I TspRI TthHB8I Van91I
XcmI XhoII