##################################
# NO CUT ENZYMES ( 58 enzymes) #
##################################
Acc65I AclI AgeI AhdI AlwNI
ApaI AscI Asp718I AspEI AviII
AvrII BbeI BbvCI BcgI BglII
BlnI BsiEI BsiWI BspDI BspLU11I
BsrBI BssHII BstEII BstPI ClaI
CpoI DrdI EagI Eam1105I EclXI
Eco52I EcoO65I FseI FspI KasI
KpnI KspI MluI NarI NheI
NotI NruI PacI PinAI PmeI
Psp1406I PvuI RsrII SacI SacII
SalI SbfI SfiI SfoI SgrAI
SphI Sse8387I StuI
###################################
# ONE CUT ENZYMES ( 37 enzymes) #
###################################
AatII 2720 GACGTC GACGT^C
AcyI 2720 GACGTC G[GA]^CG[CT]C
AflII 5618 CTTAAG C^TTAAG
AflIII 716 ACACGT A^C[GA][CT]GT
Aor51HI 629 AGCGCT AGC^GCT
BbrPI 5779 CACGTG CAC^GTG
BfrI 5618 CTTAAG C^TTAAG
BglI 1646 GCCTGTTGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 2798 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 2798 GCTCAGC GC^T[ACGT]AGC
BsaHI 2720 GACGTC G[GA]^CG[CT]C
BseRI 1858 CTCCTC GAGGAG(10/8)
Bst1107I 479 GTATAC GTA^TAC
BstAPI 5712 GCAAAGACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 4812 TTCGAA TT^CGAA
BstZ17I 479 GTATAC GTA^TAC
CelII 2798 GCTCAGC GC^T[ACGT]AGC
Eco47III 629 AGCGCT AGC^GCT
EcoNI 5760 CCTGCACCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 1428 GAATTC G^AATTC
EcoRV 4359 GATATC GAT^ATC
Hin1I 2720 GACGTC G[GA]^CG[CT]C
NaeI 2869 GCCGGC GCC^GGC
NgoMIV 2869 GCCGGC G^CCGGC
NspV 4812 TTCGAA TT^CGAA
PaeR7I 822 CTCGAG C^TCGAG
PmaCI 5779 CACGTG CAC^GTG
PmlI 5779 CACGTG CAC^GTG
PshAI 3835 GACTTGAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SexAI 4472 ACCTGGT A^CC[AT]GGT
SfuI 4812 TTCGAA TT^CGAA
SmaI 684 CCCGGG CCC^GGG
SpeI 7301 ACTAGT A^CTAGT
SwaI 5157 ATTTAAAT ATTT^AAAT
XhoI 822 CTCGAG C^TCGAG
XmaCI 684 CCCGGG C^CCGGG
XmaI 684 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 34 enzymes) #
###################################
AccIII 3584 TCCGGA T^CCGGA
6640 TCCGGA
Alw44I 1392 GTGCAC G^TGCAC
3167 GTGCAC
ApaLI 1392 GTGCAC G^TGCAC
3167 GTGCAC
AspI 2717 GACGACGTC GAC[ACGT]^[ACGT][ACGT]GTC
5631 GACAGGGTC
AvaI 684 CCCGGG C^[CT]CG[GA]G
822 CTCGAG
BamHI 1303 GGATCC G^GATCC
2395 GGATCC
BanI 1794 GGCACC G^G[CT][GA]CC
2898 GGCACC
BciVI 3428 GGATAC GTATCC(6/5)
6295 GTATCC
BsaI 639 GAGACC GGTCTC(1/5)
7433 GAGACC
BsaWI 3584 TCCGGA [AT]^CCGG[AT]
6640 TCCGGA
BseAI 3584 TCCGGA T^CCGGA
6640 TCCGGA
BsmBI 2951 CGTCTC CGTCTC(1/5)
5736 CGTCTC
BsoBI 684 CCCGGG C^[CT]CG[GA]G
822 CTCGAG
BspEI 3584 TCCGGA T^CCGGA
6640 TCCGGA
BspHI 6126 TCATGA T^CATGA
6786 TCATGA
BsrFI 2869 GCCGGC [GA]^CCGG[CT]
3679 GCCGGT
Cfr10I 2869 GCCGGC [GA]^CCGG[CT]
3679 GCCGGT
DsaI 1154 CCATGG C^C[GA][CT]GG
5984 CCATGG
EcoT22I 3212 ATGCAT ATGCA^T
6245 ATGCAT
FauI 2126 GCGGG CCCGC(4/6)
2754 GCGGG
HaeII 629 AGCGCT [GA]GCGC^[CT]
2833 GGCGCT
MfeI 1831 CAATTG C^AATTG
4563 CAATTG
MroI 3584 TCCGGA T^CCGGA
6640 TCCGGA
MunI 1831 CAATTG C^AATTG
4563 CAATTG
NcoI 1154 CCATGG C^CATGG
5984 CCATGG
NdeI 2817 CATATG CA^TATG
7352 CATATG
NsiI 3212 ATGCAT ATGCA^T
6245 ATGCAT
PflFI 2717 GACGACGTC GAC[ACGT]^[ACGT][ACGT]GTC
5631 GACAGGGTC
RcaI 6126 TCATGA T^CATGA
6786 TCATGA
SapI 1530 GAAGAGC GCTCTTC(1/4)
7119 GAAGAGC
ScaI 3361 AGTACT AGT^ACT
4392 AGTACT
SnaBI 1837 TACGTA TAC^GTA
4368 TACGTA
Tth111I 2717 GACGACGTC GAC[ACGT]^[ACGT][ACGT]GTC
5631 GACAGGGTC
XbaI 3957 TCTAGA T^CTAGA
4691 TCTAGA
#########################################
# 3 OR MORE CUT ENZYMES ( 129 enzymes) #
#########################################
AccI AccII AciI AcsI AfaI
AluI AlwI AocI ApoI AseI
AsnI Asp700I AspHI AvaII BalI
BanII BbsI BbvI BclI BcnI
BfaI BmyI BpmI BpuAI BsaAI
BsaBI BsaJI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI BsmI Bsp1286I
Bsp1407I BspMI BsrDI BsrGI BsrI
BssKI BssSI BstF5I BstNI BstUI
BstXI BstYI Bsu36I Cac8I CfoI
Cfr13I DdeI DpnII DraI DraII
DraIII EaeI EarI Eco81I EcoO109I
EcoRII EcoT14I FbaI Fnu4HI FokI
HaeIII HapII HgaI HhaI HinP1I
HincII HindII HindIII HinfI HpaI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MamI MboI MboII
MflI MluNI MnlI MscI MseI
MslI MspA1I MspI MvaI MvnI
MwoI NciI NdeII NlaIII NlaIV
NspI PflMI PleI PpuMI PshBI
PstI PvuII RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI SspBI
SspI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
Van91I XcmI XhoII XmnI