##################################
# NO CUT ENZYMES ( 80 enzymes) #
##################################
AatII AccIII AclI AgeI AhdI
Alw44I Aor51HI ApaI ApaLI AscI
Asp700I AspEI AviII BbrPI BcgI
BglI BlpI Bpu1102I BsaAI BseAI
BsiEI BsiWI BspEI BsrBI BsrFI
BssHII CelII Cfr10I CpoI DraIII
EagI Eam1105I EclXI Eco47III Eco52I
EcoRV EcoT22I FauI FseI FspI
HgaI HpaI KspI MfeI MluI
MroI MunI NaeI NgoMIV NheI
NotI NruI NsiI PacI PaeR7I
PflMI PinAI PmaCI PmeI PmlI
PshAI Psp1406I PvuI RsrII SacI
SacII SalI SapI SbfI SfiI
SgrAI SnaBI SpeI SphI Sse8387I
SwaI Van91I XcmI XhoI XmnI
###################################
# ONE CUT ENZYMES ( 48 enzymes) #
###################################
Acc65I 1749 GGTACC G^GTACC
AcyI 1213 GGCGCC G[GA]^CG[CT]C
AlwNI 2450 CAGAGTCTG CAG[ACGT][ACGT][ACGT]^CTG
Asp718I 1749 GGTACC G^GTACC
AspHI 1456 GTGCTC G[AT]GC[AT]^C
AspI 6571 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 4917 CCTAGG C^CTAGG
BamHI 1128 GGATCC G^GATCC
BanII 1842 GAGCCC G[GA]GC[CT]^C
BbeI 1213 GGCGCC GGCGC^C
BbvCI 3654 CCTCAGC CCTCAGC(-5/-2)
BlnI 4917 CCTAGG C^CTAGG
BsaHI 1213 GGCGCC G[GA]^CG[CT]C
BsaI 2355 GAGACC GGTCTC(1/5)
BsaWI 2203 TCCGGT [AT]^CCGG[AT]
BsiHKAI 1456 GTGCTC G[AT]GC[AT]^C
BsmBI 2707 CGTCTC CGTCTC(1/5)
BspDI 1498 ATCGAT AT^CGAT
BspHI 3601 TCATGA T^CATGA
BstEII 6451 GGTCACC G^GT[ACGT]ACC
BstPI 6451 GGTCACC G^GT[ACGT]ACC
ClaI 1498 ATCGAT AT^CGAT
DraII 1364 GGGACCC [GA]G^G[ACGT]CC[CT]
DrdI 2306 GACACCTAAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 2240 CCATGG C^C[GA][CT]GG
EarI 2900 CTCTTC CTCTTC(1/4)
EcoNI 4630 CCTGCCCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 1364 GGGACCC [GA]G^G[ACGT]CC[CT]
EcoO65I 6451 GGTCACC G^GT[ACGT]ACC
EcoRI 4065 GAATTC G^AATTC
HaeII 1213 GGCGCC [GA]GCGC^[CT]
Hin1I 1213 GGCGCC G[GA]^CG[CT]C
KasI 1213 GGCGCC G^GCGCC
KpnI 1749 GGTACC GGTAC^C
Ksp632I 2900 CTCTTC CTCTTC(1/4)
NarI 1213 GGCGCC GG^CGCC
NcoI 2240 CCATGG C^CATGG
NdeI 6076 CATATG CA^TATG
PflFI 6571 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PpuMI 1364 GGGACCC [GA]G^G[AT]CC[CT]
PstI 5928 CTGCAG CTGCA^G
RcaI 3601 TCATGA T^CATGA
SexAI 5715 ACCAGGT A^CC[AT]GGT
SfoI 1213 GGCGCC GGC^GCC
SmaI 6504 CCCGGG CCC^GGG
Tth111I 6571 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XmaCI 6504 CCCGGG C^CCGGG
XmaI 6504 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 20 enzymes) #
###################################
AccII 6306 CGCG CG^CG
6536 CGCG
AflIII 3205 ACATGT A^C[GA][CT]GT
6278 ACATGT
AocI 1176 CCTGAGG CC^T[ACGT]AGG
4265 CCTGAGG
AvaI 6472 CTCGGG C^[CT]CG[GA]G
6504 CCCGGG
BsmI 1391 GCATTC GAATGC(1/-1)
2870 GCATTC
BsoBI 6472 CTCGGG C^[CT]CG[GA]G
6504 CCCGGG
BspLU11I 3205 ACATGT A^CATGT
6278 ACATGT
BssSI 2341 CACGAG CACGAG(-5/-1)
6360 CACGAG
BstAPI 2743 GCACCCCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
6512 GCAGAGCTTGC
BstBI 1283 TTCGAA TT^CGAA
1719 TTCGAA
BstUI 6306 CGCG CG^CG
6536 CGCG
BstXI 3329 CCACTTCCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
6329 CCAGCACTTTGG
Bsu36I 1176 CCTGAGG CC^T[ACGT]AGG
4265 CCTGAGG
Eco81I 1176 CCTGAGG CC^T[ACGT]AGG
4265 CCTGAGG
MaeII 909 ACGT A^CGT
5029 ACGT
MvnI 6306 CGCG CG^CG
6536 CGCG
NspV 1283 TTCGAA TT^CGAA
1719 TTCGAA
SfuI 1283 TTCGAA TT^CGAA
1719 TTCGAA
StuI 1163 AGGCCT AGG^CCT
2283 AGGCCT
XbaI 4335 TCTAGA T^CTAGA
4390 TCTAGA
#########################################
# 3 OR MORE CUT ENZYMES ( 110 enzymes) #
#########################################
AccI AciI AcsI AfaI AflII
AluI AlwI ApoI AseI AsnI
AvaII BalI BanI BbsI BbvI
BciVI BclI BcnI BfaI BfrI
BglII BmyI BpmI BpuAI BsaBI
BsaJI BseRI BsgI BsiYI BslI
BsmAI BsmFI Bsp1286I Bsp1407I BspMI
BsrDI BsrGI BsrI BssKI Bst1107I
BstF5I BstNI BstYI BstZ17I Cac8I
CfoI Cfr13I DdeI DpnII DraI
EaeI EcoRII EcoT14I FbaI Fnu4HI
FokI HaeIII HapII HhaI HinP1I
HincII HindII HindIII HinfI HpaII
HphI ItaI MaeI MaeIII MamI
MboI MboII MflI MluNI MnlI
MscI MseI MslI MspA1I MspI
MvaI MwoI NciI NdeII NlaIII
NlaIV NspI PleI PshBI PvuII
RsaI Sau3AI Sau96I ScaI ScrFI
SfaNI SfcI SmlI SspBI SspI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I XhoII