##################################
# NO CUT ENZYMES ( 71 enzymes) #
##################################
AatII Acc65I AclI AcyI AflII
AgeI AhdI Alw44I Aor51HI ApaLI
AscI Asp718I AspEI AviII BamHI
BbeI BbrPI BcgI BfrI BsaHI
BsiEI BsiWI Bsp1407I BspDI BsrGI
BssHII Bst1107I BstZ17I ClaI CpoI
EagI Eam1105I EclXI Eco47III Eco52I
FseI FspI Hin1I KasI KpnI
MfeI MluI MunI NaeI NarI
NcoI NdeI NgoMIV NotI NruI
PacI PinAI PmaCI PmeI PmlI
Psp1406I PvuI RsrII SalI SbfI
SfoI SgrAI SmaI SnaBI SphI
Sse8387I SspBI SwaI XbaI XmaCI
XmaI
###################################
# ONE CUT ENZYMES ( 35 enzymes) #
###################################
AccIII 1001 TCCGGA T^CCGGA
Asp700I 2313 GAAGATCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BanI 642 GGTGCC G^G[CT][GA]CC
BclI 4509 TGATCA T^GATCA
BcnI 3102 CCCGG CC^[GC]GG
BsaAI 2882 CACGTA [CT]AC^GT[GA]
BsaBI 2903 GATATTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 1001 TCCGGA [AT]^CCGG[AT]
BseAI 1001 TCCGGA T^CCGGA
BsmBI 826 CGTCTC CGTCTC(1/5)
BspEI 1001 TCCGGA T^CCGGA
BsrBI 4796 CCGCTC CCGCTC(-3/-3)
BsrDI 1015 CATTGC GCAATG(2/0)
BsrFI 593 ACCGGC [GA]^CCGG[CT]
BssSI 4986 CTCGTG CACGAG(-5/-1)
Cfr10I 593 ACCGGC [GA]^CCGG[CT]
DraIII 1050 CACTGTGTG CAC[ACGT][ACGT][ACGT]^GTG
EcoRV 1626 GATATC GAT^ATC
FbaI 4509 TGATCA T^GATCA
HaeII 131 AGCGCC [GA]GCGC^[CT]
HgaI 825 GCGTC GACGC(5/10)
HindIII 5229 AAGCTT A^AGCTT
HpaI 2218 GTTAAC GTT^AAC
MamI 2903 GATATTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI 1001 TCCGGA T^CCGGA
NciI 3102 CCCGG CC^[GC]GG
NheI 1587 GCTAGC G^CTAGC
PshAI 1909 GACATCAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SacI 5300 GAGCTC GAGCT^C
SexAI 1484 ACCTGGT A^CC[AT]GGT
SfiI 45 GGCCGAGGCGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SpeI 3248 ACTAGT A^CTAGT
SspI 3035 AATATT AAT^ATT
XcmI 1889 CCACCAACATCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 2313 GAAGATCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 36 enzymes) #
###################################
AccI 1769 GTCTAC GT^[AC][GT]AC
2097 GTAGAC
AccII 66 CGCG CG^CG
868 CGCG
AseI 4479 ATTAAT AT^TAAT
5003 ATTAAT
AsnI 4479 ATTAAT AT^TAAT
5003 ATTAAT
AspI 3164 GACAGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
3654 GACAGAGTC
BglII 1424 AGATCT A^GATCT
2315 AGATCT
BsmI 3683 GAATGC GAATGC(1/-1)
4017 GAATGC
BspHI 1636 TCATGA T^CATGA
3253 TCATGA
BspLU11I 5565 ACATGT A^CATGT
5893 ACATGT
BspMI 1388 GCAGGT ACCTGC(4/8)
3972 GCAGGT
BstBI 28 TTCGAA TT^CGAA
1223 TTCGAA
BstEII 1391 GGTGACC G^GT[ACGT]ACC
5551 GGTAACC
BstPI 1391 GGTGACC G^GT[ACGT]ACC
5551 GGTAACC
BstUI 66 CGCG CG^CG
868 CGCG
CfoI 132 GCGC GCG^C
1386 GCGC
DrdI 681 GACAGAGGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1039 GACCCTAGGGTC
DsaI 65 CCGCGG C^C[GA][CT]GG
867 CCGCGG
EcoO65I 1391 GGTGACC G^GT[ACGT]ACC
5551 GGTAACC
EcoT22I 1569 ATGCAT ATGCA^T
5422 ATGCAT
HhaI 132 GCGC GCG^C
1386 GCGC
HinP1I 132 GCGC G^CGC
1386 GCGC
KspI 65 CCGCGG CCGC^GG
867 CCGCGG
MaeII 2883 ACGT A^CGT
6239 ACGT
MslI 5015 CACAGCCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
5372 CACCCAAATG
MvnI 66 CGCG CG^CG
868 CGCG
NsiI 1569 ATGCAT ATGCA^T
5422 ATGCAT
NspV 28 TTCGAA TT^CGAA
1223 TTCGAA
PaeR7I 3864 CTCGAG C^TCGAG
5095 CTCGAG
PflFI 3164 GACAGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
3654 GACAGAGTC
PshBI 4479 ATTAAT AT^TAAT
5003 ATTAAT
PvuII 659 CAGCTG CAG^CTG
1853 CAGCTG
RcaI 1636 TCATGA T^CATGA
3253 TCATGA
SacII 65 CCGCGG CCGC^GG
867 CCGCGG
SfuI 28 TTCGAA TT^CGAA
1223 TTCGAA
Tth111I 3164 GACAGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
3654 GACAGAGTC
XhoI 3864 CTCGAG C^TCGAG
5095 CTCGAG
#########################################
# 3 OR MORE CUT ENZYMES ( 116 enzymes) #
#########################################
AciI AcsI AfaI AflIII AluI
AlwI AlwNI AocI ApaI ApoI
AspHI AvaI AvaII AvrII BalI
BanII BbsI BbvCI BbvI BciVI
BfaI BglI BlnI BlpI BmyI
BpmI Bpu1102I BpuAI BsaI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BsrI
BssKI BstAPI BstF5I BstNI BstXI
BstYI Bsu36I Cac8I CelII Cfr13I
DdeI DpnII DraI DraII EaeI
EarI Eco81I EcoNI EcoO109I EcoRI
EcoRII EcoT14I FauI Fnu4HI FokI
HaeIII HapII HincII HindII HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeIII MboI MboII MflI MluNI
MnlI MscI MseI MspA1I MspI
MvaI MwoI NdeII NlaIII NlaIV
NspI PflMI PleI PpuMI PstI
RsaI SapI Sau3AI Sau96I ScaI
ScrFI SfaNI SfcI SmlI StuI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I Van91I
XhoII