##################################
# NO CUT ENZYMES ( 84 enzymes) #
##################################
AatII Acc65I AccII AccIII AgeI
AhdI Alw44I AocI ApaI ApaLI
AscI Asp718I AspEI AviII AvrII
BamHI BbeI BbrPI BciVI BglII
BlnI BlpI Bpu1102I BsaWI BseAI
BsgI BsiEI BsiWI BsmBI Bsp1407I
BspEI BsrBI BsrGI BssHII BstEII
BstPI BstUI Bsu36I CelII CpoI
DraII DraIII EagI Eam1105I EclXI
Eco52I Eco81I EcoO109I EcoO65I EcoRV
FseI FspI HpaI KasI KpnI
KspI MfeI MluI MroI MunI
MvnI NarI NotI NruI PacI
PinAI PmaCI PmeI PmlI PpuMI
PvuI PvuII RsrII SacII SalI
SapI SbfI SfiI SfoI SmaI
Sse8387I SspBI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 54 enzymes) #
###################################
AclI 6129 AACGTT AA^CGTT
AcyI 123 GGCGTC G[GA]^CG[CT]C
AlwI 348 GGATC GGATC(4/5)
Aor51HI 4799 AGCGCT AGC^GCT
AspI 120 GACGGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 3282 CTCGAG C^[CT]CG[GA]G
BanI 6192 GGTGCC G^G[CT][GA]CC
BcgI 49 CGAGAGAGATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglI 10 GCCGTTGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaAI 4987 TACGTA [CT]AC^GT[GA]
BsaHI 123 GGCGTC G[GA]^CG[CT]C
BsaI 3628 GGTCTC GGTCTC(1/5)
BsoBI 3282 CTCGAG C^[CT]CG[GA]G
BsrFI 198 GCCGGC [GA]^CCGG[CT]
BssSI 2755 CACGAG CACGAG(-5/-1)
Bst1107I 4077 GTATAC GTA^TAC
BstBI 5672 TTCGAA TT^CGAA
BstYI 348 GGATCT [GA]^GATC[CT]
BstZ17I 4077 GTATAC GTA^TAC
CfoI 4800 GCGC GCG^C
Cfr10I 198 GCCGGC [GA]^CCGG[CT]
EarI 3442 CTCTTC CTCTTC(1/4)
Eco47III 4799 AGCGCT AGC^GCT
EcoT22I 3851 ATGCAT ATGCA^T
HaeII 4799 AGCGCT [GA]GCGC^[CT]
HhaI 4800 GCGC GCG^C
Hin1I 123 GGCGTC G[GA]^CG[CT]C
HinP1I 4800 GCGC G^CGC
HindIII 2415 AAGCTT A^AGCTT
Ksp632I 3442 CTCTTC CTCTTC(1/4)
MflI 348 GGATCT [GA]^GATC[CT]
MspA1I 6188 CAGCGG C[AC]G^C[GT]G
NaeI 198 GCCGGC GCC^GGC
NcoI 1537 CCATGG C^CATGG
NgoMIV 198 GCCGGC G^CCGGC
NheI 534 GCTAGC G^CTAGC
NsiI 3851 ATGCAT ATGCA^T
NspV 5672 TTCGAA TT^CGAA
PaeR7I 3282 CTCGAG C^TCGAG
PflFI 120 GACGGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshAI 4217 GACACTTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Psp1406I 6129 AACGTT AA^CGTT
SacI 3696 GAGCTC GAGCT^C
SexAI 4807 ACCTGGT A^CC[AT]GGT
SfuI 5672 TTCGAA TT^CGAA
SgrAI 197 CGCCGGCG C[GA]^CCGG[CT]G
SnaBI 4987 TACGTA TAC^GTA
SphI 5324 GCATGC GCATG^C
SwaI 5818 ATTTAAAT ATTT^AAAT
Tth111I 120 GACGGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 3827 TCTAGA T^CTAGA
XcmI 2736 CCATGTGAATAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 3282 CTCGAG C^TCGAG
XhoII 348 GGATCT [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AccI 3298 GTAGAC GT^[AC][GT]AC
4077 GTATAC
AflII 984 CTTAAG C^TTAAG
6255 CTTAAG
AflIII 142 ACATGT A^C[GA][CT]GT
5455 ACATGT
AseI 2560 ATTAAT AT^TAAT
2968 ATTAAT
AsnI 2560 ATTAAT AT^TAAT
2968 ATTAAT
BbvCI 529 CCTCAGC CCTCAGC(-5/-2)
5358 GCTGAGG
BclI 4695 TGATCA T^GATCA
5873 TGATCA
BfrI 984 CTTAAG C^TTAAG
6255 CTTAAG
BsaBI 873 GATGAAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
5086 GATATTTATC
BsmAI 3629 GTCTC GTCTC(1/5)
5432 GTCTC
BspDI 2441 ATCGAT AT^CGAT
4176 ATCGAT
BspLU11I 142 ACATGT A^CATGT
5455 ACATGT
BstXI 184 CCACCACCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
6125 CCAAAACGTTGG
ClaI 2441 ATCGAT AT^CGAT
4176 ATCGAT
FbaI 4695 TGATCA T^GATCA
5873 TGATCA
HgaI 124 GCGTC GACGC(5/10)
243 GCGTC
MamI 873 GATGAAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
5086 GATATTTATC
NdeI 3231 CATATG CA^TATG
3923 CATATG
PleI 173 GACTC GAGTC(4/5)
545 GACTC
PshBI 2560 ATTAAT AT^TAAT
2968 ATTAAT
ScaI 288 AGTACT AGT^ACT
2941 AGTACT
SpeI 1857 ACTAGT A^CTAGT
2944 ACTAGT
#########################################
# 3 OR MORE CUT ENZYMES ( 98 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwNI
ApoI Asp700I AspHI AvaII BalI
BanII BbsI BbvI BcnI BfaI
BmyI BpmI BpuAI BsaJI BseRI
BsiHKAI BsiYI BslI BsmFI BsmI
Bsp1286I BspHI BspMI BsrDI BsrI
BssKI BstAPI BstF5I BstNI Cac8I
Cfr13I DdeI DpnII DraI DrdI
DsaI EaeI EcoNI EcoRI EcoRII
EcoT14I FauI Fnu4HI FokI HaeIII
HapII HincII HindII HinfI HpaII
HphI ItaI MaeI MaeII MaeIII
MboI MboII MluNI MnlI MscI
MseI MslI MspI MvaI MwoI
NciI NdeII NlaIII NlaIV NspI
PflMI PstI RcaI RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
SspI StuI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I Van91I XmnI