##################################
# NO CUT ENZYMES ( 46 enzymes) #
##################################
Acc65I AclI AscI AseI AsnI
Asp718I AviII BciVI BsaBI BsiWI
BsrBI BssHII BstBI CpoI EcoNI
EcoRI FseI FspI HindIII HpaI
KpnI KspI MamI MfeI MluI
MunI NdeI NheI NotI NspV
PacI PflMI PmeI PshAI PshBI
Psp1406I RsrII SacII SapI SexAI
SfuI SgrAI SpeI SwaI Van91I
XbaI
###################################
# ONE CUT ENZYMES ( 38 enzymes) #
###################################
AatII 5919 GACGTC GACGT^C
AccIII 800 TCCGGA T^CCGGA
AflII 5457 CTTAAG C^TTAAG
AgeI 3083 ACCGGT A^CCGGT
Alw44I 1723 GTGCAC G^TGCAC
AocI 1663 CCTAAGG CC^T[ACGT]AGG
ApaI 2523 GGGCCC GGGCC^C
ApaLI 1723 GTGCAC G^TGCAC
AvrII 2730 CCTAGG C^CTAGG
BamHI 1836 GGATCC G^GATCC
BclI 4669 TGATCA T^GATCA
BfrI 5457 CTTAAG C^TTAAG
BlnI 2730 CCTAGG C^CTAGG
BseAI 800 TCCGGA T^CCGGA
BspEI 800 TCCGGA T^CCGGA
Bst1107I 3566 GTATAC GTA^TAC
BstEII 423 GGTGACC G^GT[ACGT]ACC
BstPI 423 GGTGACC G^GT[ACGT]ACC
BstZ17I 3566 GTATAC GTA^TAC
Bsu36I 1663 CCTAAGG CC^T[ACGT]AGG
DraIII 3177 CACCATGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 1141 GACTTTGATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 1663 CCTAAGG CC^T[ACGT]AGG
EcoO65I 423 GGTGACC G^GT[ACGT]ACC
FbaI 4669 TGATCA T^GATCA
MroI 800 TCCGGA T^CCGGA
NaeI 2237 GCCGGC GCC^GGC
NcoI 3360 CCATGG C^CATGG
NgoMIV 2237 GCCGGC G^CCGGC
NruI 79 TCGCGA TCG^CGA
PinAI 3083 ACCGGT A^CCGGT
PvuI 1174 CGATCG CGAT^CG
SalI 2700 GTCGAC G^TCGAC
SbfI 3187 CCTGCAGG CCTGCA^GG
SfiI 3059 GGCCCGTGCGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SphI 5554 GCATGC GCATG^C
Sse8387I 3187 CCTGCAGG CCTGCA^GG
SspI 2027 AATATT AAT^ATT
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AccI 2700 GTCGAC GT^[AC][GT]AC
3566 GTATAC
AspI 1179 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
4465 GACACTGTC
BbeI 2844 GGCGCC GGCGC^C
3031 GGCGCC
BbrPI 3257 CACGTG CAC^GTG
4584 CACGTG
BbvCI 549 CCTCAGC CCTCAGC(-5/-2)
3324 GCTGAGG
BcgI 2127 CGAGGCTATTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
2943 CGACCCCGATGC
BglII 3635 AGATCT A^GATCT
5944 AGATCT
BsaWI 800 TCCGGA [AT]^CCGG[AT]
3083 ACCGGT
BsgI 2800 CTGCAC GTGCAG(16/14)
3317 GTGCAG
BsmBI 5921 CGTCTC CGTCTC(1/5)
4092 GAGACG
BsrDI 1230 CATTGC GCAATG(2/0)
2354 CATTGC
BstAPI 2802 GCACGAGTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
5518 GCAGAGGCTGC
EagI 3067 CGGCCG C^GGCCG
3122 CGGCCG
EclXI 3067 CGGCCG C^GGCCG
3122 CGGCCG
Eco52I 3067 CGGCCG C^GGCCG
3122 CGGCCG
EcoRV 1600 GATATC GAT^ATC
1686 GATATC
KasI 2844 GGCGCC G^GCGCC
3031 GGCGCC
NarI 2844 GGCGCC GG^CGCC
3031 GGCGCC
PflFI 1179 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
4465 GACACTGTC
PmaCI 3257 CACGTG CAC^GTG
4584 CACGTG
PmlI 3257 CACGTG CAC^GTG
4584 CACGTG
ScaI 143 AGTACT AGT^ACT
641 AGTACT
SfoI 2844 GGCGCC GGC^GCC
3031 GGCGCC
SnaBI 3523 TACGTA TAC^GTA
3569 TACGTA
Tth111I 1179 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
4465 GACACTGTC
XcmI 3235 CCAGGGTGGCCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
3400 CCAGAGGAGTGCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 148 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AflIII AhdI AluI AlwI AlwNI
Aor51HI ApoI Asp700I AspEI AspHI
AvaI AvaII BalI BanI BanII
BbsI BbvI BcnI BfaI BglI
BlpI BmyI BpmI Bpu1102I BpuAI
BsaAI BsaHI BsaI BsaJI BseRI
BsiEI BsiHKAI BsiYI BslI BsmAI
BsmFI BsmI BsoBI Bsp1286I Bsp1407I
BspDI BspHI BspLU11I BspMI BsrFI
BsrGI BsrI BssKI BssSI BstF5I
BstNI BstUI BstXI BstYI Cac8I
CelII CfoI Cfr10I Cfr13I ClaI
DdeI DpnII DraI DraII DsaI
EaeI Eam1105I EarI Eco47III EcoO109I
EcoRII EcoT14I EcoT22I FauI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI Hin1I HinP1I HincII HindII
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MluNI MnlI MscI MseI
MslI MspA1I MspI MvaI MvnI
MwoI NciI NdeII NlaIII NlaIV
NsiI NspI PaeR7I PleI PpuMI
PstI PvuII RcaI RsaI SacI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmaI SmlI SspBI StuI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I XhoI XhoII
XmaCI XmaI XmnI