##################################
# NO CUT ENZYMES ( 55 enzymes) #
##################################
AatII Acc65I AclI AflII AgeI
AhdI AscI AseI AsnI Asp718I
AspEI AspI BciVI BfrI BsaBI
BsiWI BsmBI BspDI BspHI BspLU11I
BsrDI BssHII Bst1107I BstZ17I ClaI
CpoI Eam1105I EcoRV HpaI KpnI
MamI MluI NdeI NotI NruI
PacI PaeR7I PflFI PinAI PmeI
PshAI PshBI Psp1406I RcaI RsrII
SbfI SgrAI SnaBI SpeI Sse8387I
SspI SwaI Tth111I XbaI XhoI
###################################
# ONE CUT ENZYMES ( 39 enzymes) #
###################################
AccIII 3536 TCCGGA T^CCGGA
AflIII 4118 ACGTGT A^C[GA][CT]GT
AvrII 4264 CCTAGG C^CTAGG
BclI 1788 TGATCA T^GATCA
BglII 2448 AGATCT A^GATCT
BlnI 4264 CCTAGG C^CTAGG
BsaWI 3536 TCCGGA [AT]^CCGG[AT]
BseAI 3536 TCCGGA T^CCGGA
Bsp1407I 4121 TGTACA T^GTACA
BspEI 3536 TCCGGA T^CCGGA
BsrGI 4121 TGTACA T^GTACA
BssSI 4375 CTCGTG CACGAG(-5/-1)
BstBI 2320 TTCGAA TT^CGAA
DraI 4611 TTTAAA TTT^AAA
DrdI 3782 GACTGCAGGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 913 CGGCCG C^GGCCG
EclXI 913 CGGCCG C^GGCCG
Eco52I 913 CGGCCG C^GGCCG
EcoRI 1289 GAATTC G^AATTC
EcoT22I 1875 ATGCAT ATGCA^T
FbaI 1788 TGATCA T^GATCA
FseI 2712 GGCCGGCC GGCCGG^CC
HindIII 1029 AAGCTT A^AGCTT
MfeI 5359 CAATTG C^AATTG
MroI 3536 TCCGGA T^CCGGA
MunI 5359 CAATTG C^AATTG
NheI 4978 GCTAGC G^CTAGC
NsiI 1875 ATGCAT ATGCA^T
NspV 2320 TTCGAA TT^CGAA
PflMI 3949 CCACAGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PvuI 2283 CGATCG CGAT^CG
SalI 605 GTCGAC G^TCGAC
ScaI 960 AGTACT AGT^ACT
SexAI 3237 ACCAGGT A^CC[AT]GGT
SfiI 824 GGCCCCGAGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfuI 2320 TTCGAA TT^CGAA
SspBI 4121 TGTACA T^GTACA
TfiI 3462 GATTC G^A[AT]TC
Van91I 3949 CCACAGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AcsI 1289 GAATTC [GA]^AATT[CT]
4624 AAATTT
ApoI 1289 GAATTC [GA]^AATT[CT]
4624 AAATTT
Asp700I 127 GAAGAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1952 GAACAGTTTC
AviII 2681 TGCGCA TGC^GCA
5135 TGCGCA
BamHI 1248 GGATCC G^GATCC
5271 GGATCC
BbrPI 4099 CACGTG CAC^GTG
4117 CACGTG
BbsI 250 GAAGAC GAAGAC(2/6)
1975 GTCTTC
BpuAI 250 GAAGAC GAAGAC(2/6)
1975 GTCTTC
BsaAI 4099 CACGTG [CT]AC^GT[GA]
4117 CACGTG
BsiEI 913 CGGCCG CG[GA][CT]^CG
2283 CGATCG
BsmI 5433 GAATGC GAATGC(1/-1)
1748 GCATTC
FspI 2681 TGCGCA TGC^GCA
5135 TGCGCA
HincII 605 GTCGAC GT[CT]^[GA]AC
5493 GTCAAC
HindII 605 GTCGAC GT[CT]^[GA]AC
5493 GTCAAC
KspI 3900 CCGCGG CCGC^GG
4001 CCGCGG
PmaCI 4099 CACGTG CAC^GTG
4117 CACGTG
PmlI 4099 CACGTG CAC^GTG
4117 CACGTG
SacII 3900 CCGCGG CCGC^GG
4001 CCGCGG
SapI 3069 GAAGAGC GCTCTTC(1/4)
205 GCTCTTC
SphI 293 GCATGC GCATG^C
1877 GCATGC
XmnI 127 GAAGAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1952 GAACAGTTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 143 enzymes) #
#########################################
AccI AccII AciI AcyI AfaI
AluI Alw44I AlwI AlwNI AocI
Aor51HI ApaI ApaLI AspHI AvaI
AvaII BalI BanI BanII BbeI
BbvCI BbvI BcgI BcnI BfaI
BglI BlpI BmyI BpmI Bpu1102I
BsaHI BsaI BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BspMI BsrBI BsrFI
BsrI BssKI BstAPI BstEII BstF5I
BstNI BstPI BstUI BstXI BstYI
Bsu36I Cac8I CelII CfoI Cfr10I
Cfr13I DdeI DpnII DraII DraIII
DsaI EaeI EarI Eco47III Eco81I
EcoNI EcoO109I EcoO65I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI Hin1I HinP1I
HinfI HpaII HphI ItaI KasI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MluNI MnlI MscI
MseI MslI MspA1I MspI MvaI
MvnI MwoI NaeI NarI NciI
NcoI NdeII NgoMIV NlaIII NlaIV
NspI PleI PpuMI PstI PvuII
RsaI SacI Sau3AI Sau96I ScrFI
SfaNI SfcI SfoI SmaI SmlI
StuI StyI TaqI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I XcmI
XhoII XmaCI XmaI