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# NO CUT ENZYMES ( 54 enzymes) #
##################################
AatII Acc65I AccI AclI AflII
AscI Asp718I AviII AvrII BfrI
BlnI BsiEI BsiWI Bsp1407I BspHI
BspLU11I BsrGI BssHII Bst1107I BstAPI
BstBI BstZ17I EagI EclXI Eco52I
EcoT22I FspI HindIII HpaI KpnI
MluI NheI NotI NruI NsiI
NspV PaeR7I PmeI PshAI Psp1406I
PvuI RcaI SalI SbfI SfiI
SfuI SgrAI SpeI SphI Sse8387I
SspBI SwaI XbaI XhoI
###################################
# ONE CUT ENZYMES ( 48 enzymes) #
###################################
AccIII 693 TCCGGA T^CCGGA
AflIII 4835 ACACGT A^C[GA][CT]GT
AgeI 3960 ACCGGT A^CCGGT
Alw44I 3483 GTGCAC G^TGCAC
Aor51HI 60 AGCGCT AGC^GCT
ApaLI 3483 GTGCAC G^TGCAC
AseI 5349 ATTAAT AT^TAAT
AsnI 5349 ATTAAT AT^TAAT
AspI 3576 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
BalI 1032 TGGCCA TGG^CCA
BamHI 4636 GGATCC G^GATCC
BbrPI 4836 CACGTG CAC^GTG
BciVI 3651 GTATCC GTATCC(6/5)
BclI 236 TGATCA T^GATCA
BglII 3564 AGATCT A^GATCT
BsaBI 2122 GATGGACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseAI 693 TCCGGA T^CCGGA
BspDI 1310 ATCGAT AT^CGAT
BspEI 693 TCCGGA T^CCGGA
BssSI 5116 CTCGTG CACGAG(-5/-1)
ClaI 1310 ATCGAT AT^CGAT
CpoI 2070 CGGTCCG CG^G[AT]CCG
Eco47III 60 AGCGCT AGC^GCT
EcoRI 1178 GAATTC G^AATTC
EcoRV 3475 GATATC GAT^ATC
FbaI 236 TGATCA T^GATCA
FseI 808 GGCCGGCC GGCCGG^CC
MamI 2122 GATGGACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 4751 CAATTG C^AATTG
MluNI 1032 TGGCCA TGG^CCA
MroI 693 TCCGGA T^CCGGA
MscI 1032 TGGCCA TGG^CCA
MslI 2962 CATCCCCGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MunI 4751 CAATTG C^AATTG
NdeI 1686 CATATG CA^TATG
PacI 5346 TTAATTAA TTAAT^TAA
PflFI 3576 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PinAI 3960 ACCGGT A^CCGGT
PmaCI 4836 CACGTG CAC^GTG
PmlI 4836 CACGTG CAC^GTG
PshBI 5349 ATTAAT AT^TAAT
RsrII 2070 CGGTCCG CG^G[AT]CCG
SacI 3774 GAGCTC GAGCT^C
SapI 3221 GCTCTTC GCTCTTC(1/4)
SexAI 4657 ACCTGGT A^CC[AT]GGT
SnaBI 4491 TACGTA TAC^GTA
SspI 5292 AATATT AAT^ATT
Tth111I 3576 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
###################################
# TWO CUT ENZYMES ( 20 enzymes) #
###################################
AocI 1545 CCTCAGG CC^T[ACGT]AGG
2685 CCTCAGG
BbvCI 1046 CCTCAGC CCTCAGC(-5/-2)
1863 CCTCAGC
BlpI 442 GCTGAGC GC^T[ACGT]AGC
1516 GCTGAGC
Bpu1102I 442 GCTGAGC GC^T[ACGT]AGC
1516 GCTGAGC
BsmBI 3037 CGTCTC CGTCTC(1/5)
3270 GAGACG
BsmI 1831 GAATGC GAATGC(1/-1)
1490 GCATTC
BstEII 3343 GGTGACC G^GT[ACGT]ACC
4441 GGTGACC
BstPI 3343 GGTGACC G^GT[ACGT]ACC
4441 GGTGACC
Bsu36I 1545 CCTCAGG CC^T[ACGT]AGG
2685 CCTCAGG
CelII 442 GCTGAGC GC^T[ACGT]AGC
1516 GCTGAGC
DrdI 992 GACAGGACTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3431 GACCAGGGAGTC
Eco81I 1545 CCTCAGG CC^T[ACGT]AGG
2685 CCTCAGG
EcoO65I 3343 GGTGACC G^GT[ACGT]ACC
4441 GGTGACC
HincII 2621 GTCAAC GT[CT]^[GA]AC
4756 GTTGAC
HindII 2621 GTCAAC GT[CT]^[GA]AC
4756 GTTGAC
NcoI 3096 CCATGG C^CATGG
3913 CCATGG
ScaI 1161 AGTACT AGT^ACT
5258 AGTACT
SmaI 2055 CCCGGG CCC^GGG
2666 CCCGGG
XmaCI 2055 CCCGGG C^CCGGG
2666 CCCGGG
XmaI 2055 CCCGGG C^CCGGG
2666 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 136 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AhdI AluI AlwI AlwNI ApaI
ApoI Asp700I AspEI AspHI AvaI
AvaII BanI BanII BbeI BbsI
BbvI BcgI BcnI BfaI BglI
BmyI BpmI BpuAI BsaAI BsaHI
BsaI BsaJI BsaWI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BspMI BsrBI BsrDI
BsrFI BsrI BssKI BstF5I BstNI
BstUI BstXI BstYI Cac8I CfoI
Cfr10I Cfr13I DdeI DpnII DraI
DraII DraIII DsaI EaeI Eam1105I
EarI EcoNI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI Hin1I HinP1I
HinfI HpaII HphI ItaI KasI
Ksp632I KspI MaeI MaeII MaeIII
MboI MboII MflI MnlI MseI
MspA1I MspI MvaI MvnI MwoI
NaeI NarI NciI NdeII NgoMIV
NlaIII NlaIV NspI PflMI PleI
PpuMI PstI PvuII RsaI SacII
Sau3AI Sau96I ScrFI SfaNI SfcI
SfoI SmlI StuI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I Van91I XcmI XhoII
XmnI