##################################
# NO CUT ENZYMES ( 46 enzymes) #
##################################
AccIII AgeI AscI AviII BglII
BsaBI BsaWI BseAI BsiWI Bsp1407I
BspDI BspEI BsrGI BssHII BstBI
ClaI CpoI DrdI EcoRV EcoT22I
FseI FspI HpaI MamI MfeI
MluI MroI MunI NdeI NotI
NruI NsiI NspV PacI PinAI
PmeI PshAI RsrII SalI SapI
SfuI SgrAI SnaBI SspBI SwaI
XbaI
###################################
# ONE CUT ENZYMES ( 30 enzymes) #
###################################
AatII 2565 GACGTC GACGT^C
AclI 3028 AACGTT AA^CGTT
AflII 4604 CTTAAG C^TTAAG
AflIII 4210 ACATGT A^C[GA][CT]GT
Aor51HI 5504 AGCGCT AGC^GCT
Asp700I 252 GAAGCGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BciVI 5022 GTATCC GTATCC(6/5)
BfrI 4604 CTTAAG C^TTAAG
BsmBI 4243 GAGACG CGTCTC(1/5)
BsmI 2791 GAATGC GAATGC(1/-1)
BspLU11I 4210 ACATGT A^CATGT
BsrDI 4012 GCAATG GCAATG(2/0)
Bst1107I 3244 GTATAC GTA^TAC
BstAPI 4280 GCATTTATTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstZ17I 3244 GTATAC GTA^TAC
Eco47III 5504 AGCGCT AGC^GCT
EcoRI 6121 GAATTC G^AATTC
HincII 1249 GTCAAC GT[CT]^[GA]AC
HindII 1249 GTCAAC GT[CT]^[GA]AC
NcoI 5026 CCATGG C^CATGG
NheI 6362 GCTAGC G^CTAGC
Psp1406I 3028 AACGTT AA^CGTT
PvuI 111 CGATCG CGAT^CG
SbfI 6383 CCTGCAGG CCTGCA^GG
ScaI 2943 AGTACT AGT^ACT
SfiI 3143 GGCCAGCATGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SpeI 2924 ACTAGT A^CTAGT
SphI 4745 GCATGC GCATG^C
Sse8387I 6383 CCTGCAGG CCTGCA^GG
XmnI 252 GAAGCGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 37 enzymes) #
###################################
Acc65I 700 GGTACC G^GTACC
3905 GGTACC
AhdI 577 GACATTCGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1422 GACCCTGGGTC
AseI 5238 ATTAAT AT^TAAT
5512 ATTAAT
AsnI 5238 ATTAAT AT^TAAT
5512 ATTAAT
Asp718I 700 GGTACC G^GTACC
3905 GGTACC
AspEI 577 GACATTCGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1422 GACCCTGGGTC
AvrII 3272 CCTAGG C^CTAGG
4579 CCTAGG
BamHI 2423 GGATCC G^GATCC
4678 GGATCC
BbeI 1848 GGCGCC GGCGC^C
1861 GGCGCC
BbvCI 5731 GCTGAGG CCTCAGC(-5/-2)
5866 GCTGAGG
BlnI 3272 CCTAGG C^CTAGG
4579 CCTAGG
BspHI 999 TCATGA T^CATGA
3853 TCATGA
BssSI 1747 CACGAG CACGAG(-5/-1)
6428 CACGAG
BstEII 151 GGTGACC G^GT[ACGT]ACC
3615 GGTCACC
BstPI 151 GGTGACC G^GT[ACGT]ACC
3615 GGTCACC
DraI 3677 TTTAAA TTT^AAA
6346 TTTAAA
EagI 1363 CGGCCG C^GGCCG
5044 CGGCCG
Eam1105I 577 GACATTCGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1422 GACCCTGGGTC
EclXI 1363 CGGCCG C^GGCCG
5044 CGGCCG
Eco52I 1363 CGGCCG C^GGCCG
5044 CGGCCG
EcoO65I 151 GGTGACC G^GT[ACGT]ACC
3615 GGTCACC
HindIII 5160 AAGCTT A^AGCTT
5473 AAGCTT
KasI 1848 GGCGCC G^GCGCC
1861 GGCGCC
KpnI 700 GGTACC GGTAC^C
3905 GGTACC
KspI 376 CCGCGG CCGC^GG
1734 CCGCGG
NarI 1848 GGCGCC GG^CGCC
1861 GGCGCC
PaeR7I 2936 CTCGAG C^TCGAG
4880 CTCGAG
PflMI 1746 CCACGAGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3965 CCAAGGGGTGG
PshBI 5238 ATTAAT AT^TAAT
5512 ATTAAT
RcaI 999 TCATGA T^CATGA
3853 TCATGA
SacI 9 GAGCTC GAGCT^C
685 GAGCTC
SacII 376 CCGCGG CCGC^GG
1734 CCGCGG
SexAI 1064 ACCAGGT A^CC[AT]GGT
5366 ACCAGGT
SfoI 1848 GGCGCC GGC^GCC
1861 GGCGCC
SspI 3503 AATATT AAT^ATT
4305 AATATT
Van91I 1746 CCACGAGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3965 CCAAGGGGTGG
XhoI 2936 CTCGAG C^TCGAG
4880 CTCGAG
#########################################
# 3 OR MORE CUT ENZYMES ( 145 enzymes) #
#########################################
AccI AccII AciI AcsI AcyI
AfaI AluI Alw44I AlwI AlwNI
AocI ApaI ApaLI ApoI AspHI
AspI AvaI AvaII BalI BanI
BanII BbrPI BbsI BbvI BcgI
BclI BcnI BfaI BglI BlpI
BmyI BpmI Bpu1102I BpuAI BsaAI
BsaHI BsaI BsaJI BseRI BsgI
BsiEI BsiHKAI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BspMI BsrBI
BsrFI BsrI BssKI BstF5I BstNI
BstUI BstXI BstYI Bsu36I Cac8I
CelII CfoI Cfr10I Cfr13I DdeI
DpnII DraII DraIII DsaI EaeI
EarI Eco81I EcoNI EcoO109I EcoRII
EcoT14I FauI FbaI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
Hin1I HinP1I HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MluNI MnlI
MscI MseI MslI MspA1I MspI
MvaI MvnI MwoI NaeI NciI
NdeII NgoMIV NlaIII NlaIV NspI
PflFI PleI PmaCI PmlI PpuMI
PstI PvuII RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SmaI SmlI
StuI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI Tth111I
TthHB8I XcmI XhoII XmaCI XmaI