##################################
# NO CUT ENZYMES ( 52 enzymes) #
##################################
AatII Acc65I AclI AgeI AhdI
AscI AseI AsnI Asp718I AspEI
AvrII BbrPI BlnI BsiWI BsmBI
BsmI BssHII BssSI Bst1107I BstBI
BstZ17I CpoI DrdI Eam1105I EcoRI
EcoRV HindIII KpnI MfeI MunI
NdeI NheI NotI NruI NspV
PacI PaeR7I PinAI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PvuI
RsrII SapI SfuI SgrAI SnaBI
SpeI XhoI
###################################
# ONE CUT ENZYMES ( 32 enzymes) #
###################################
AccIII 348 TCCGGA T^CCGGA
AflII 4830 CTTAAG C^TTAAG
Aor51HI 4822 AGCGCT AGC^GCT
AviII 1324 TGCGCA TGC^GCA
BclI 2047 TGATCA T^GATCA
BfrI 4830 CTTAAG C^TTAAG
BsaBI 1402 GATCAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseAI 348 TCCGGA T^CCGGA
BspDI 2030 ATCGAT AT^CGAT
BspEI 348 TCCGGA T^CCGGA
BspHI 1338 TCATGA T^CATGA
ClaI 2030 ATCGAT AT^CGAT
DraI 5848 TTTAAA TTT^AAA
DraIII 3546 CACTGGGTG CAC[ACGT][ACGT][ACGT]^GTG
Eco47III 4822 AGCGCT AGC^GCT
EcoT22I 5423 ATGCAT ATGCA^T
FbaI 2047 TGATCA T^GATCA
FspI 1324 TGCGCA TGC^GCA
HpaI 3480 GTTAAC GTT^AAC
MamI 1402 GATCAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluI 1752 ACGCGT A^CGCGT
MroI 348 TCCGGA T^CCGGA
NsiI 5423 ATGCAT ATGCA^T
RcaI 1338 TCATGA T^CATGA
SacI 1352 GAGCTC GAGCT^C
SalI 2728 GTCGAC G^TCGAC
SbfI 4272 CCTGCAGG CCTGCA^GG
ScaI 2115 AGTACT AGT^ACT
SexAI 3375 ACCAGGT A^CC[AT]GGT
Sse8387I 4272 CCTGCAGG CCTGCA^GG
SwaI 5847 ATTTAAAT ATTT^AAAT
XbaI 5113 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 30 enzymes) #
###################################
Asp700I 520 GAAGCAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
5291 GAAGGGTTTC
AspI 1262 GACAATGTC GAC[ACGT]^[ACGT][ACGT]GTC
4332 GACCCCGTC
BamHI 2131 GGATCC G^GATCC
5670 GGATCC
BcgI 1132 GCATGAGAATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
1843 GCAGCGGACTCG
BciVI 412 GGATAC GTATCC(6/5)
4975 GGATAC
BglII 1242 AGATCT A^GATCT
1407 AGATCT
BlpI 2714 GCTCAGC GC^T[ACGT]AGC
3860 GCTGAGC
Bpu1102I 2714 GCTCAGC GC^T[ACGT]AGC
3860 GCTGAGC
BsaAI 773 TACGTG [CT]AC^GT[GA]
1518 TACGTG
BsaI 1094 GAGACC GGTCTC(1/5)
2612 GAGACC
BsaWI 348 TCCGGA [AT]^CCGG[AT]
2808 ACCGGA
Bsp1407I 3900 TGTACA T^GTACA
3912 TGTACA
BspLU11I 135 ACATGT A^CATGT
2415 ACATGT
BsrGI 3900 TGTACA T^GTACA
3912 TGTACA
BstEII 3358 GGTGACC G^GT[ACGT]ACC
4457 GGTGACC
BstPI 3358 GGTGACC G^GT[ACGT]ACC
4457 GGTGACC
CelII 2714 GCTCAGC GC^T[ACGT]AGC
3860 GCTGAGC
EcoNI 3370 CCTGTACCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
4663 CCTACTTCAGG
EcoO65I 3358 GGTGACC G^GT[ACGT]ACC
4457 GGTGACC
HincII 2728 GTCGAC GT[CT]^[GA]AC
3480 GTTAAC
HindII 2728 GTCGAC GT[CT]^[GA]AC
3480 GTTAAC
PflFI 1262 GACAATGTC GAC[ACGT]^[ACGT][ACGT]GTC
4332 GACCCCGTC
SfiI 1661 GGCCGGGCTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
1825 GGCCATGCTGGCC
SmaI 1452 CCCGGG CCC^GGG
2862 CCCGGG
SphI 903 GCATGC GCATG^C
2710 GCATGC
SspBI 3900 TGTACA T^GTACA
3912 TGTACA
Tth111I 1262 GACAATGTC GAC[ACGT]^[ACGT][ACGT]GTC
4332 GACCCCGTC
XmaCI 1452 CCCGGG C^CCGGG
2862 CCCGGG
XmaI 1452 CCCGGG C^CCGGG
2862 CCCGGG
XmnI 520 GAAGCAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
5291 GAAGGGTTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 144 enzymes) #
#########################################
AccI AccII AciI AcsI AcyI
AfaI AflIII AluI Alw44I AlwI
AlwNI AocI ApaI ApaLI ApoI
AspHI AvaI AvaII BalI BanI
BanII BbeI BbsI BbvCI BbvI
BcnI BfaI BglI BmyI BpmI
BpuAI BsaHI BsaJI BseRI BsgI
BsiEI BsiHKAI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BspMI BsrBI
BsrDI BsrFI BsrI BssKI BstAPI
BstF5I BstNI BstUI BstXI BstYI
Bsu36I Cac8I CfoI Cfr10I Cfr13I
DdeI DpnII DraII DsaI EaeI
EagI EarI EclXI Eco52I Eco81I
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI FseI HaeII HaeIII HapII
HgaI HhaI Hin1I HinP1I HinfI
HpaII HphI ItaI KasI Ksp632I
KspI MaeI MaeII MaeIII MboI
MboII MflI MluNI MnlI MscI
MseI MslI MspA1I MspI MvaI
MvnI MwoI NaeI NarI NciI
NcoI NdeII NgoMIV NlaIII NlaIV
NspI PflMI PleI PpuMI PstI
PvuII RsaI SacII Sau3AI Sau96I
ScrFI SfaNI SfcI SfoI SmlI
SspI StuI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I Van91I XcmI XhoII